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Diuretic and hypotensive effect of morelloflavone from Garcinia dulcis in two-kidneys-one-clip (2K1C) hypertensive rat

Morelloflavone, a biflavonoid from Southeast Asian folk medical plant Garcinia dulcis, possesses powerful antioxidant activity both in vivo and in vitro. We aimed to evaluate the hypotensive and diuretic effect of morelloflavone in two-kidneys-one-clip (2K1C) renovascular hypertensive rats together...

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Main Authors: Nattaya Thongsepee,, Wilawan Mahabusarakam,, Siriphun Hiranyachattada,
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2017
Online Access:http://journalarticle.ukm.my/11374/1/17%20Nattaya%20Thongsepee.pdf
http://journalarticle.ukm.my/11374/
http://www.ukm.my/jsm/english_journals/vol46num9_2017/contentsVol46num9_2017.html
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spelling my-ukm.journal.113742018-02-15T05:16:12Z http://journalarticle.ukm.my/11374/ Diuretic and hypotensive effect of morelloflavone from Garcinia dulcis in two-kidneys-one-clip (2K1C) hypertensive rat Nattaya Thongsepee, Wilawan Mahabusarakam, Siriphun Hiranyachattada, Morelloflavone, a biflavonoid from Southeast Asian folk medical plant Garcinia dulcis, possesses powerful antioxidant activity both in vivo and in vitro. We aimed to evaluate the hypotensive and diuretic effect of morelloflavone in two-kidneys-one-clip (2K1C) renovascular hypertensive rats together with its vasorelaxant mechanism. Male Wistar rats (175±4 g) were undergone 2K1C and sham operation (SO) (n=6, each group). Four weeks after the rats were anesthetized and clearance markers (0.5% para-aminohippuric acid and 1% inulin) were given via a jugular vein (1.6 mL/h/100 g BW) to estimate renal blood flow (RBF) and glomerular filtration rate. The arterial blood pressure (AP) was monitored via a carotid artery and urine samples were collected. After equilibration, either morelloflavone or vehicle (DMSO) was given (0.1 mg/kg BW+5 μg/min/kg BW). Baroreflex sensitivity (BRS) (ΔHR/ΔMAP) was performed by an intravenous injection of 1, 2, 4, 8, 16 and 32 μg/kg BW phenylephrine (PE) or sodium nitroprusside. The PE-preconstricted isolated thoracic aortic rings (intact and denuded) relaxation were experimented using organ bath technique by cumulative additions of morelloflavaone (10-13-10-5 M) in the presence of specific vasorelaxant inhibitors and expressed as %relaxation from pre-contraction tension. In 2K1C rats, morelloflavone significantly lowered mean AP, increased RBF and increased urine flow rate when compared to vehicle control (138±6 vs. 152±1 mmHg, 3.44±0.49 vs. 2.29±0.25 mL/min/g KW and 42.0±9.4 vs. 18.2±3.9 μL/min/g KW, p<0.05), respectively and also restored the blunt BRS. Nitric oxide signaling pathway triggered by morelloflavone might be responsible for its diuretic and hypotensive effect. Penerbit Universiti Kebangsaan Malaysia 2017-09 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/11374/1/17%20Nattaya%20Thongsepee.pdf Nattaya Thongsepee, and Wilawan Mahabusarakam, and Siriphun Hiranyachattada, (2017) Diuretic and hypotensive effect of morelloflavone from Garcinia dulcis in two-kidneys-one-clip (2K1C) hypertensive rat. Sains Malaysiana, 46 (9). pp. 1479-1490. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol46num9_2017/contentsVol46num9_2017.html
institution Universiti Kebangsaan Malaysia
building Perpustakaan Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Morelloflavone, a biflavonoid from Southeast Asian folk medical plant Garcinia dulcis, possesses powerful antioxidant activity both in vivo and in vitro. We aimed to evaluate the hypotensive and diuretic effect of morelloflavone in two-kidneys-one-clip (2K1C) renovascular hypertensive rats together with its vasorelaxant mechanism. Male Wistar rats (175±4 g) were undergone 2K1C and sham operation (SO) (n=6, each group). Four weeks after the rats were anesthetized and clearance markers (0.5% para-aminohippuric acid and 1% inulin) were given via a jugular vein (1.6 mL/h/100 g BW) to estimate renal blood flow (RBF) and glomerular filtration rate. The arterial blood pressure (AP) was monitored via a carotid artery and urine samples were collected. After equilibration, either morelloflavone or vehicle (DMSO) was given (0.1 mg/kg BW+5 μg/min/kg BW). Baroreflex sensitivity (BRS) (ΔHR/ΔMAP) was performed by an intravenous injection of 1, 2, 4, 8, 16 and 32 μg/kg BW phenylephrine (PE) or sodium nitroprusside. The PE-preconstricted isolated thoracic aortic rings (intact and denuded) relaxation were experimented using organ bath technique by cumulative additions of morelloflavaone (10-13-10-5 M) in the presence of specific vasorelaxant inhibitors and expressed as %relaxation from pre-contraction tension. In 2K1C rats, morelloflavone significantly lowered mean AP, increased RBF and increased urine flow rate when compared to vehicle control (138±6 vs. 152±1 mmHg, 3.44±0.49 vs. 2.29±0.25 mL/min/g KW and 42.0±9.4 vs. 18.2±3.9 μL/min/g KW, p<0.05), respectively and also restored the blunt BRS. Nitric oxide signaling pathway triggered by morelloflavone might be responsible for its diuretic and hypotensive effect.
format Article
author Nattaya Thongsepee,
Wilawan Mahabusarakam,
Siriphun Hiranyachattada,
spellingShingle Nattaya Thongsepee,
Wilawan Mahabusarakam,
Siriphun Hiranyachattada,
Diuretic and hypotensive effect of morelloflavone from Garcinia dulcis in two-kidneys-one-clip (2K1C) hypertensive rat
author_facet Nattaya Thongsepee,
Wilawan Mahabusarakam,
Siriphun Hiranyachattada,
author_sort Nattaya Thongsepee,
title Diuretic and hypotensive effect of morelloflavone from Garcinia dulcis in two-kidneys-one-clip (2K1C) hypertensive rat
title_short Diuretic and hypotensive effect of morelloflavone from Garcinia dulcis in two-kidneys-one-clip (2K1C) hypertensive rat
title_full Diuretic and hypotensive effect of morelloflavone from Garcinia dulcis in two-kidneys-one-clip (2K1C) hypertensive rat
title_fullStr Diuretic and hypotensive effect of morelloflavone from Garcinia dulcis in two-kidneys-one-clip (2K1C) hypertensive rat
title_full_unstemmed Diuretic and hypotensive effect of morelloflavone from Garcinia dulcis in two-kidneys-one-clip (2K1C) hypertensive rat
title_sort diuretic and hypotensive effect of morelloflavone from garcinia dulcis in two-kidneys-one-clip (2k1c) hypertensive rat
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2017
url http://journalarticle.ukm.my/11374/1/17%20Nattaya%20Thongsepee.pdf
http://journalarticle.ukm.my/11374/
http://www.ukm.my/jsm/english_journals/vol46num9_2017/contentsVol46num9_2017.html
_version_ 1643738470446792704
score 13.211869

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