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A protocol for rapid and high-frequency in vitro propagation of solanum nigrum L.

Solanum nigrum L. is a species highly valued for its medicinal properties. In the present study, an efficient propagation system was established by using five explants of S. nigrum namely, roots, leaves, rooted hypocotyls, nodal segments and petioles. Various types of plant growth regulators (PGRs)...

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Main Authors: Zou, Li-Juan, Yang, Jing-Tian, Wu, Qing-Gui
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2017
Online Access:http://journalarticle.ukm.my/11137/1/03%20Li-Juan%20Zou.pdf
http://journalarticle.ukm.my/11137/
http://www.ukm.my/jsm/malay_journals/jilid46bil8_2017/KandunganJilid46Bil8_2017.html
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spelling my-ukm.journal.111372017-12-21T05:18:11Z http://journalarticle.ukm.my/11137/ A protocol for rapid and high-frequency in vitro propagation of solanum nigrum L. Zou, Li-Juan Yang, Jing-Tian Wu, Qing-Gui Solanum nigrum L. is a species highly valued for its medicinal properties. In the present study, an efficient propagation system was established by using five explants of S. nigrum namely, roots, leaves, rooted hypocotyls, nodal segments and petioles. Various types of plant growth regulators (PGRs) were used to determine the most effective hormone combination for callus induction and organogenesis. Zeatin (ZT), thidiazuron (TDZ), kinetin (Kin) and indole-3-acetic acid (IAA) were found to induce multiple shoots. Shoot organogenesis was induced in the five explants. The highest mean for number of shoots per petioles (31.54±5.76) and rooted hypocotyls (44.00±1.51) with a 100% induction rate was obtained on Murashige and Skoog (MS) medium fortified with 0.4 mg/L IAA and 3.0 mg/L ZT. MS medium containing 0.4 mg/L IAA and 3.0 mg/L TDZ was found to be optimal for shoot regeneration of roots, leaves and nodal segments. The highest regeneration frequency (100%) with mean numbers of shoots equal to 38.77±6.87 for roots, 42.73±7.75 for leaves and 56.73±7.98 for nodal segments was produced. Regenerated shoots rooted effectively on half-strength MS medium and acclimatized successfully in soil with a 100% survival rate and normal growth. The protocol can be used for the large-scale propagation of S. nigrum to meet the increasing demand of commercial cultivation. Penerbit Universiti Kebangsaan Malaysia 2017-08 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/11137/1/03%20Li-Juan%20Zou.pdf Zou, Li-Juan and Yang, Jing-Tian and Wu, Qing-Gui (2017) A protocol for rapid and high-frequency in vitro propagation of solanum nigrum L. Sains Malaysiana, 46 (8). pp. 1183-1189. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid46bil8_2017/KandunganJilid46Bil8_2017.html
institution Universiti Kebangsaan Malaysia
building Perpustakaan Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Solanum nigrum L. is a species highly valued for its medicinal properties. In the present study, an efficient propagation system was established by using five explants of S. nigrum namely, roots, leaves, rooted hypocotyls, nodal segments and petioles. Various types of plant growth regulators (PGRs) were used to determine the most effective hormone combination for callus induction and organogenesis. Zeatin (ZT), thidiazuron (TDZ), kinetin (Kin) and indole-3-acetic acid (IAA) were found to induce multiple shoots. Shoot organogenesis was induced in the five explants. The highest mean for number of shoots per petioles (31.54±5.76) and rooted hypocotyls (44.00±1.51) with a 100% induction rate was obtained on Murashige and Skoog (MS) medium fortified with 0.4 mg/L IAA and 3.0 mg/L ZT. MS medium containing 0.4 mg/L IAA and 3.0 mg/L TDZ was found to be optimal for shoot regeneration of roots, leaves and nodal segments. The highest regeneration frequency (100%) with mean numbers of shoots equal to 38.77±6.87 for roots, 42.73±7.75 for leaves and 56.73±7.98 for nodal segments was produced. Regenerated shoots rooted effectively on half-strength MS medium and acclimatized successfully in soil with a 100% survival rate and normal growth. The protocol can be used for the large-scale propagation of S. nigrum to meet the increasing demand of commercial cultivation.
format Article
author Zou, Li-Juan
Yang, Jing-Tian
Wu, Qing-Gui
spellingShingle Zou, Li-Juan
Yang, Jing-Tian
Wu, Qing-Gui
A protocol for rapid and high-frequency in vitro propagation of solanum nigrum L.
author_facet Zou, Li-Juan
Yang, Jing-Tian
Wu, Qing-Gui
author_sort Zou, Li-Juan
title A protocol for rapid and high-frequency in vitro propagation of solanum nigrum L.
title_short A protocol for rapid and high-frequency in vitro propagation of solanum nigrum L.
title_full A protocol for rapid and high-frequency in vitro propagation of solanum nigrum L.
title_fullStr A protocol for rapid and high-frequency in vitro propagation of solanum nigrum L.
title_full_unstemmed A protocol for rapid and high-frequency in vitro propagation of solanum nigrum L.
title_sort protocol for rapid and high-frequency in vitro propagation of solanum nigrum l.
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2017
url http://journalarticle.ukm.my/11137/1/03%20Li-Juan%20Zou.pdf
http://journalarticle.ukm.my/11137/
http://www.ukm.my/jsm/malay_journals/jilid46bil8_2017/KandunganJilid46Bil8_2017.html
_version_ 1643738382553055232
score 13.211869

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