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Neuroprotective effect of rice and corn extracts against H2O2-induced neurotoxicity in HT22 murine hippocampal neuronal cells

Oxidative stress-induced neuronal damage, mediated by the cellular accumulation of hydrogen peroxide (H2O2), was reported to be involved in neurodegenerative diseases. Rice and corn may serve as sources of antioxidants with the presence of phytochemicals such as melatonin and tryptophan, for fightin...

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Main Authors: Chumpiya S,, Plaingarm W,, Tewin Tencomnao,
Format: Article
Language:English
Published: Faculty of Medicine, Universiti Kebangsaan Malaysia 2016
Online Access:http://journalarticle.ukm.my/10839/1/5_chumpiya_et_al_pdf_21167.pdf
http://journalarticle.ukm.my/10839/
http://www.medicineandhealthukm.com/toc/11/2
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spelling my-ukm.journal.108392017-10-12T09:18:32Z http://journalarticle.ukm.my/10839/ Neuroprotective effect of rice and corn extracts against H2O2-induced neurotoxicity in HT22 murine hippocampal neuronal cells Chumpiya S, Plaingarm W, Tewin Tencomnao, Oxidative stress-induced neuronal damage, mediated by the cellular accumulation of hydrogen peroxide (H2O2), was reported to be involved in neurodegenerative diseases. Rice and corn may serve as sources of antioxidants with the presence of phytochemicals such as melatonin and tryptophan, for fighting against reactive oxygen species (ROS). Melatonin are known to upregulate the expression of brain-derived neurotrophic factor (BDNF) gene for enhancement of the nerve cell function and mediation of the anti-aging effect of the brain cells. The present study aimed at determining the effect of white rice, brown rice, black glutinous rice, sweet corn and baby corn extracts against H2O2-induced neurotoxicity and their underlying mechanisms in the mouse hippocampal HT22 cells. Following pretreatment of the HT22 cells with individual extract for 24 hrs and subsequent challenge with H2O2 for 24 hrs, cell viability, apoptosis, intracellular ROS and BDNF gene expression were measured. Rice and corn extracts were evaluated for their antioxidant activities and the levels of tryptophan and melatonin. Characteristics of H2O2-induced neurotoxicity included decreased cell viability, increased cellular ROS and decreased BDNF expression. Pretreatment with rice and corn extracts significantly attenuated H2O2-induced neurotoxicity, and also significantly decreased ROS production. Pretreatment with these extracts could also upregulate the expression of BDNF mRNA and protein, which may be contributed by their melatonin and tryptophan contents. Therefore, rice and corn extracts may protect HT22 cells against H2O2-induced neurotoxicity by inhibition of ROS production and modulation of BDNF expression. Faculty of Medicine, Universiti Kebangsaan Malaysia 2016 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/10839/1/5_chumpiya_et_al_pdf_21167.pdf Chumpiya S, and Plaingarm W, and Tewin Tencomnao, (2016) Neuroprotective effect of rice and corn extracts against H2O2-induced neurotoxicity in HT22 murine hippocampal neuronal cells. Medicine & Health, 11 (2). pp. 151-170. ISSN 1823-2140 http://www.medicineandhealthukm.com/toc/11/2
institution Universiti Kebangsaan Malaysia
building Perpustakaan Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Oxidative stress-induced neuronal damage, mediated by the cellular accumulation of hydrogen peroxide (H2O2), was reported to be involved in neurodegenerative diseases. Rice and corn may serve as sources of antioxidants with the presence of phytochemicals such as melatonin and tryptophan, for fighting against reactive oxygen species (ROS). Melatonin are known to upregulate the expression of brain-derived neurotrophic factor (BDNF) gene for enhancement of the nerve cell function and mediation of the anti-aging effect of the brain cells. The present study aimed at determining the effect of white rice, brown rice, black glutinous rice, sweet corn and baby corn extracts against H2O2-induced neurotoxicity and their underlying mechanisms in the mouse hippocampal HT22 cells. Following pretreatment of the HT22 cells with individual extract for 24 hrs and subsequent challenge with H2O2 for 24 hrs, cell viability, apoptosis, intracellular ROS and BDNF gene expression were measured. Rice and corn extracts were evaluated for their antioxidant activities and the levels of tryptophan and melatonin. Characteristics of H2O2-induced neurotoxicity included decreased cell viability, increased cellular ROS and decreased BDNF expression. Pretreatment with rice and corn extracts significantly attenuated H2O2-induced neurotoxicity, and also significantly decreased ROS production. Pretreatment with these extracts could also upregulate the expression of BDNF mRNA and protein, which may be contributed by their melatonin and tryptophan contents. Therefore, rice and corn extracts may protect HT22 cells against H2O2-induced neurotoxicity by inhibition of ROS production and modulation of BDNF expression.
format Article
author Chumpiya S,
Plaingarm W,
Tewin Tencomnao,
spellingShingle Chumpiya S,
Plaingarm W,
Tewin Tencomnao,
Neuroprotective effect of rice and corn extracts against H2O2-induced neurotoxicity in HT22 murine hippocampal neuronal cells
author_facet Chumpiya S,
Plaingarm W,
Tewin Tencomnao,
author_sort Chumpiya S,
title Neuroprotective effect of rice and corn extracts against H2O2-induced neurotoxicity in HT22 murine hippocampal neuronal cells
title_short Neuroprotective effect of rice and corn extracts against H2O2-induced neurotoxicity in HT22 murine hippocampal neuronal cells
title_full Neuroprotective effect of rice and corn extracts against H2O2-induced neurotoxicity in HT22 murine hippocampal neuronal cells
title_fullStr Neuroprotective effect of rice and corn extracts against H2O2-induced neurotoxicity in HT22 murine hippocampal neuronal cells
title_full_unstemmed Neuroprotective effect of rice and corn extracts against H2O2-induced neurotoxicity in HT22 murine hippocampal neuronal cells
title_sort neuroprotective effect of rice and corn extracts against h2o2-induced neurotoxicity in ht22 murine hippocampal neuronal cells
publisher Faculty of Medicine, Universiti Kebangsaan Malaysia
publishDate 2016
url http://journalarticle.ukm.my/10839/1/5_chumpiya_et_al_pdf_21167.pdf
http://journalarticle.ukm.my/10839/
http://www.medicineandhealthukm.com/toc/11/2
_version_ 1643738276145659904
score 13.211869

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