Cover Image

Characterisation of Metagenomic Bougainvillea Soil DNA Through rDNA PCR Amplification

Rhizosphere is an important part of soil ecosystem, being a habitat for a vast amount of bacteria that is influenced by root secretions, which contribute to the richness of bacteria in the soil. Conventional culturing often is not able to simulate the environmental settings microorganisms are adapte...

Full description

Saved in:
Bibliographic Details
Main Author: Ganesan, Aruna
Format: Thesis
Language:English
Published: 2017
Subjects:
QR Microbiology
Online Access:http://eprints.intimal.edu.my/948/1/124.pdf
http://eprints.intimal.edu.my/948/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my-inti-eprints.948
record_format eprints
spelling my-inti-eprints.9482017-11-14T08:17:17Z http://eprints.intimal.edu.my/948/ Characterisation of Metagenomic Bougainvillea Soil DNA Through rDNA PCR Amplification Ganesan, Aruna QR Microbiology Rhizosphere is an important part of soil ecosystem, being a habitat for a vast amount of bacteria that is influenced by root secretions, which contribute to the richness of bacteria in the soil. Conventional culturing often is not able to simulate the environmental settings microorganisms are adapted to, thus posing barrier for the advancement in environmental and clinical microbiology. Metagenomics evades this unculturability by analysing genomic variability of bacteria in environmental samples and this process starts off with DNA extraction. The aim of this research is to select several soil samples, extract the DNA from the microbiota within, and analysed. Soil samples were collected under bougainvillea shrubs for DNA isolation. All the samples were analysed using agarose gel electrophoresis, restriction digestion, polymerase chain reaction and spectrophotometer. CTAB DNA extraction produced intact high molecular weight band on agarose gel suggesting intact metagenomics DNA was isolated and had moderate A260/A280 ratio indicating moderate purity from protein contamination. From the sequencing data, identification of plant growth promoting microorganisms can be done which are beneficial to the field of agriculture. 2017 Thesis NonPeerReviewed text en http://eprints.intimal.edu.my/948/1/124.pdf Ganesan, Aruna (2017) Characterisation of Metagenomic Bougainvillea Soil DNA Through rDNA PCR Amplification. Other thesis, INTI International University.
institution INTI International University
building INTI Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider INTI International University
content_source INTI Institutional Repository
url_provider http://eprints.intimal.edu.my
language English
topic QR Microbiology
spellingShingle QR Microbiology
Ganesan, Aruna
Characterisation of Metagenomic Bougainvillea Soil DNA Through rDNA PCR Amplification
description Rhizosphere is an important part of soil ecosystem, being a habitat for a vast amount of bacteria that is influenced by root secretions, which contribute to the richness of bacteria in the soil. Conventional culturing often is not able to simulate the environmental settings microorganisms are adapted to, thus posing barrier for the advancement in environmental and clinical microbiology. Metagenomics evades this unculturability by analysing genomic variability of bacteria in environmental samples and this process starts off with DNA extraction. The aim of this research is to select several soil samples, extract the DNA from the microbiota within, and analysed. Soil samples were collected under bougainvillea shrubs for DNA isolation. All the samples were analysed using agarose gel electrophoresis, restriction digestion, polymerase chain reaction and spectrophotometer. CTAB DNA extraction produced intact high molecular weight band on agarose gel suggesting intact metagenomics DNA was isolated and had moderate A260/A280 ratio indicating moderate purity from protein contamination. From the sequencing data, identification of plant growth promoting microorganisms can be done which are beneficial to the field of agriculture.
format Thesis
author Ganesan, Aruna
author_facet Ganesan, Aruna
author_sort Ganesan, Aruna
title Characterisation of Metagenomic Bougainvillea Soil DNA Through rDNA PCR Amplification
title_short Characterisation of Metagenomic Bougainvillea Soil DNA Through rDNA PCR Amplification
title_full Characterisation of Metagenomic Bougainvillea Soil DNA Through rDNA PCR Amplification
title_fullStr Characterisation of Metagenomic Bougainvillea Soil DNA Through rDNA PCR Amplification
title_full_unstemmed Characterisation of Metagenomic Bougainvillea Soil DNA Through rDNA PCR Amplification
title_sort characterisation of metagenomic bougainvillea soil dna through rdna pcr amplification
publishDate 2017
url http://eprints.intimal.edu.my/948/1/124.pdf
http://eprints.intimal.edu.my/948/
_version_ 1644541347989815296
score 13.211869

Similar Items