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Bioremediation Potential of Fungi Isolates on Copper

Nowadays, heavy metal pollution had endangered not only living organism, but also health of natural environment. This phenomenon is usually caused by anthropogenic activities, for example heavy metal discharge to water source and mining, which causes damages to air, water as well as land areas. Howe...

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Main Author: Hoh, Xin Han
Format: Thesis
Language:English
Published: 2016
Subjects:
TP Chemical technology
Online Access:http://eprints.intimal.edu.my/895/1/119.pdf
http://eprints.intimal.edu.my/895/
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spelling my-inti-eprints.8952017-08-24T09:04:55Z http://eprints.intimal.edu.my/895/ Bioremediation Potential of Fungi Isolates on Copper Hoh, Xin Han TP Chemical technology Nowadays, heavy metal pollution had endangered not only living organism, but also health of natural environment. This phenomenon is usually caused by anthropogenic activities, for example heavy metal discharge to water source and mining, which causes damages to air, water as well as land areas. However, this problem is treatable either by biological or chemical approaches. One of the most common and cost-effective of biological method to treat the contamination sites is bioremediation. In this research paper, fungi isolates were used to conduct the study, namely nycoremediation. The aim of this study is to study the potential of Aspergillus niger, Aspergillus tamari, Simplicillium subtropicum and Fusarium solani in bioremediating copper metal, and hence determine their bioremediation efficiency. From the stock culture of the four fungi, they were inoculated to fresh potato dextrose agar (PDA) plate to obtain pure culture. With copper concentration range from 0ppm (control) to 200ppm, pure culture is inoculated into solution containing potato dextrose broth and copper sulphate (II) salt to allow growth and metal uptake. Triplicates of medium were done to provide consistent results. After cultivation period of 7-10 days, biomasses were harvested and homogenized. The biomasses were sent to inductively-coupled plasma mass spectrometry (ICP-MS) to determine metal uptake rate after measuring the dry weight of fungi biomasses. The results obtained showed S. subtropicum grew best, with 0.806g dry biomass weight, at 50 ppm of CuSO4. Whereas for result ICP-MS, S. subtropicum showed outstanding biosorption capacity among other isolates. After analysis of the results obtained, it can concluded that Simplicillium subtropicum was the best isolates in bioremediation of copper with respect to ICP-MS result and growth rate measured by mass 2016 Thesis NonPeerReviewed text en http://eprints.intimal.edu.my/895/1/119.pdf Hoh, Xin Han (2016) Bioremediation Potential of Fungi Isolates on Copper. Other thesis, INTI International University.
institution INTI International University
building INTI Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider INTI International University
content_source INTI Institutional Repository
url_provider http://eprints.intimal.edu.my
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Hoh, Xin Han
Bioremediation Potential of Fungi Isolates on Copper
description Nowadays, heavy metal pollution had endangered not only living organism, but also health of natural environment. This phenomenon is usually caused by anthropogenic activities, for example heavy metal discharge to water source and mining, which causes damages to air, water as well as land areas. However, this problem is treatable either by biological or chemical approaches. One of the most common and cost-effective of biological method to treat the contamination sites is bioremediation. In this research paper, fungi isolates were used to conduct the study, namely nycoremediation. The aim of this study is to study the potential of Aspergillus niger, Aspergillus tamari, Simplicillium subtropicum and Fusarium solani in bioremediating copper metal, and hence determine their bioremediation efficiency. From the stock culture of the four fungi, they were inoculated to fresh potato dextrose agar (PDA) plate to obtain pure culture. With copper concentration range from 0ppm (control) to 200ppm, pure culture is inoculated into solution containing potato dextrose broth and copper sulphate (II) salt to allow growth and metal uptake. Triplicates of medium were done to provide consistent results. After cultivation period of 7-10 days, biomasses were harvested and homogenized. The biomasses were sent to inductively-coupled plasma mass spectrometry (ICP-MS) to determine metal uptake rate after measuring the dry weight of fungi biomasses. The results obtained showed S. subtropicum grew best, with 0.806g dry biomass weight, at 50 ppm of CuSO4. Whereas for result ICP-MS, S. subtropicum showed outstanding biosorption capacity among other isolates. After analysis of the results obtained, it can concluded that Simplicillium subtropicum was the best isolates in bioremediation of copper with respect to ICP-MS result and growth rate measured by mass
format Thesis
author Hoh, Xin Han
author_facet Hoh, Xin Han
author_sort Hoh, Xin Han
title Bioremediation Potential of Fungi Isolates on Copper
title_short Bioremediation Potential of Fungi Isolates on Copper
title_full Bioremediation Potential of Fungi Isolates on Copper
title_fullStr Bioremediation Potential of Fungi Isolates on Copper
title_full_unstemmed Bioremediation Potential of Fungi Isolates on Copper
title_sort bioremediation potential of fungi isolates on copper
publishDate 2016
url http://eprints.intimal.edu.my/895/1/119.pdf
http://eprints.intimal.edu.my/895/
_version_ 1644541334062628864
score 13.211869

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