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Response of Selected Carnivorous Plants to Thidiazuron and RNA Extraction of Drosera x tokaiensis

Carnivorous plants are undergoing extinction due to their wide exploitation for use in medical and horticultural industry, thus require in vitro culturing as an alternative method to preserve them and increase their market availability without endangering them. A range of concentrations of thidiazur...

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Main Author: Lim, Ying Houng
Format: Thesis
Language:English
Published: 2015
Subjects:
TP Chemical technology
Online Access:http://eprints.intimal.edu.my/439/1/BBTEI%20-%2081.pdf
http://eprints.intimal.edu.my/439/
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spelling my-inti-eprints.4392016-08-01T07:00:33Z http://eprints.intimal.edu.my/439/ Response of Selected Carnivorous Plants to Thidiazuron and RNA Extraction of Drosera x tokaiensis Lim, Ying Houng TP Chemical technology Carnivorous plants are undergoing extinction due to their wide exploitation for use in medical and horticultural industry, thus require in vitro culturing as an alternative method to preserve them and increase their market availability without endangering them. A range of concentrations of thidiazuron (TDZ) at 0.00, 0.01, 0.05, 0.20, 1.00 and 5.00 mg/L was tested on Drosera x tokaiensis, Drosera burmanii and Dionea muscipula to determine the optimum concentration for inducing somatic embryogenesis (SE) in each of the species. The cultures were treated for 30 days with one subculture at day 15. The response from TDZ treatment on the explants of the selected carnivorous plant species were determined by calculating the total number of somatic embryos formed. The results obtained were tested by using Analysis of Variance (ANOVA) and Dunnett’s T3 test to detect the significant difference caused by the different concentration of TDZ. Different concentrations of TDZ resulted in a significant difference among explants of D. x iokaiensi and no significant difference among explants of D. burmanii. In particular, 0.01 mg/L of TDZ induced the highest number of somatic embryo in explants of L). x iokaiensis and 5 mg/L of TDZ induced highest number of somatic embryo in explants of D. burrnanii. All explants of D. muscipula showed no induction of somatic embryo. To further confirm that somatic embryogenesis is taking place, detection of the expression of embryogenesis-related genes can be done. RNA extraction was performed for the assay in this study using the Analytik Jena RNA extraction Kit and cetyltrimethyl ammonium bromide (CTAB). Extracted total RNA from D. x tokaienis were charactcrised by using agarose gel electrophoresis and UV spectrophotometry. RNA was successfully extracted by using the CTAB method. Two bands of 28S and I 8S were visible in the intact total RNA samples. The total yield and quality of RNA extracted lower than expected probably due to pipetting error during 70% ethanol aspirating step. 2015 Thesis NonPeerReviewed text en http://eprints.intimal.edu.my/439/1/BBTEI%20-%2081.pdf Lim, Ying Houng (2015) Response of Selected Carnivorous Plants to Thidiazuron and RNA Extraction of Drosera x tokaiensis. Other thesis, INTI International University.
institution INTI International University
building INTI Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider INTI International University
content_source INTI Institutional Repository
url_provider http://eprints.intimal.edu.my
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Lim, Ying Houng
Response of Selected Carnivorous Plants to Thidiazuron and RNA Extraction of Drosera x tokaiensis
description Carnivorous plants are undergoing extinction due to their wide exploitation for use in medical and horticultural industry, thus require in vitro culturing as an alternative method to preserve them and increase their market availability without endangering them. A range of concentrations of thidiazuron (TDZ) at 0.00, 0.01, 0.05, 0.20, 1.00 and 5.00 mg/L was tested on Drosera x tokaiensis, Drosera burmanii and Dionea muscipula to determine the optimum concentration for inducing somatic embryogenesis (SE) in each of the species. The cultures were treated for 30 days with one subculture at day 15. The response from TDZ treatment on the explants of the selected carnivorous plant species were determined by calculating the total number of somatic embryos formed. The results obtained were tested by using Analysis of Variance (ANOVA) and Dunnett’s T3 test to detect the significant difference caused by the different concentration of TDZ. Different concentrations of TDZ resulted in a significant difference among explants of D. x iokaiensi and no significant difference among explants of D. burmanii. In particular, 0.01 mg/L of TDZ induced the highest number of somatic embryo in explants of L). x iokaiensis and 5 mg/L of TDZ induced highest number of somatic embryo in explants of D. burrnanii. All explants of D. muscipula showed no induction of somatic embryo. To further confirm that somatic embryogenesis is taking place, detection of the expression of embryogenesis-related genes can be done. RNA extraction was performed for the assay in this study using the Analytik Jena RNA extraction Kit and cetyltrimethyl ammonium bromide (CTAB). Extracted total RNA from D. x tokaienis were charactcrised by using agarose gel electrophoresis and UV spectrophotometry. RNA was successfully extracted by using the CTAB method. Two bands of 28S and I 8S were visible in the intact total RNA samples. The total yield and quality of RNA extracted lower than expected probably due to pipetting error during 70% ethanol aspirating step.
format Thesis
author Lim, Ying Houng
author_facet Lim, Ying Houng
author_sort Lim, Ying Houng
title Response of Selected Carnivorous Plants to Thidiazuron and RNA Extraction of Drosera x tokaiensis
title_short Response of Selected Carnivorous Plants to Thidiazuron and RNA Extraction of Drosera x tokaiensis
title_full Response of Selected Carnivorous Plants to Thidiazuron and RNA Extraction of Drosera x tokaiensis
title_fullStr Response of Selected Carnivorous Plants to Thidiazuron and RNA Extraction of Drosera x tokaiensis
title_full_unstemmed Response of Selected Carnivorous Plants to Thidiazuron and RNA Extraction of Drosera x tokaiensis
title_sort response of selected carnivorous plants to thidiazuron and rna extraction of drosera x tokaiensis
publishDate 2015
url http://eprints.intimal.edu.my/439/1/BBTEI%20-%2081.pdf
http://eprints.intimal.edu.my/439/
_version_ 1644541208463147008
score 13.211869

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