DNA Barcoding Hibiscus Plant Species Using rbcL Gene and trnH-psbA Intergenic Spacer Region
Plants have enabled humans to advance by providing us with food, medicine and raw materials for producing goods. In order to gain those benefits from plants, we need to be able to accurately identify and differentiate those plaits at a species level. DNA barcoding uses particular regions of the DNA...
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| Main Author: | |
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| Format: | Thesis |
| Language: | English |
| Published: |
2015
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| Subjects: | |
| Online Access: | http://eprints.intimal.edu.my/428/1/BBTEI%20-%2070.pdf http://eprints.intimal.edu.my/428/ |
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| Summary: | Plants have enabled humans to advance by providing us with food, medicine and raw materials for producing goods. In order to gain those benefits from plants, we need to be able to accurately identify and differentiate those plaits at a species level. DNA barcoding uses particular regions of the DNA to identify species, for plants rbcL and maiK are the standard genes for barcoding. The objective of the thesis was to test the rbcL and trnH-psbA combination on Hibiscus, a genus of medicinal and culinary importance. Leaf samples were collected from Hibiscus schizopetalus, Hibiscus esculentus and hibiscus sabdariffa. DNA was extracted from the leaf samples; rbcL and trnH-psbA were amplified via PCR. 9 samples were successfully amplified and sequenced. The results revealed trnH-pshA gave identification similar to morphological identification unlike, rbcL which is a relatively easier loci to successfully perform PCR on compared to trnH-psbA. Other DNA barcode combination should be tested on hibiscus species. |
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