Staff View: Comparative degradation between heavy and light crude oil mediated by nitrogen‐induced cell‐surface hydrophobicity

Comparative degradation between heavy and light crude oil mediated by nitrogen‐induced cell‐surface hydrophobicity

A bacterial strain UKMP‐10M2 isolated from a Malaysian petroleum refinery was able to degrade 84% of heavy Khafji sour crude and 68% of light Tapis sweet crude within seven days. Analysis of gas chromatography‐flame ionization detector chromatograms show the strain UKMP‐10M2 degraded up to 90% prist...

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Main Authors:	Kok, Kee W, Hamzah, A
Format:	Article
Published:	SpringerLink 2018
Subjects:	HB Economic Theory HF Commerce
Online Access:	http://eprints.intimal.edu.my/1163/ https://doi.org/10.1002/rem.21574
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spelling	my-inti-eprints.11632020-10-20T04:07:10Z http://eprints.intimal.edu.my/1163/ Comparative degradation between heavy and light crude oil mediated by nitrogen‐induced cell‐surface hydrophobicity Kok, Kee W Hamzah, A HB Economic Theory HF Commerce A bacterial strain UKMP‐10M2 isolated from a Malaysian petroleum refinery was able to degrade 84% of heavy Khafji sour crude and 68% of light Tapis sweet crude within seven days. Analysis of gas chromatography‐flame ionization detector chromatograms show the strain UKMP‐10M2 degraded up to 90% pristane and 50% phytane in heavy crude, but significantly lower pristane (50%) and phytane (30%) were degraded from the light crude. A mixture of aliphatic hexadecane and three‐ring phenanthrene better supported the growth of isolate UKMP‐10M2 compared to using phenanthrene alone, suggesting cometabolism influenced how crude oil with different individual hydrocarbon contents affected the degradation. Peptone as the source of nitrogen increases the emulsifying index in UKMP‐10M2 exposed to heavy Khafji sour crude 20% higher than in light Tapis sweet crude. However, BATH assay showed the same nitrogen source increases bacterial cell surface hydrophobicity of UKMP‐10M2 up to 14% higher in light Tapis crude oil compared to heavy Khafji. This study suggest the nitrogen source plays a decisive role in elevating UKMP‐10M2 bacterial cells hydrophobicity, and in correlation with types of crude oil. Phylogenetic tree analysis based on 16S rDNA sequence results identified the strain to be Rhodococcus ruber. SpringerLink 2018 Article PeerReviewed Kok, Kee W and Hamzah, A (2018) Comparative degradation between heavy and light crude oil mediated by nitrogen‐induced cell‐surface hydrophobicity. Remediation, 28 (4). ISSN 10515658 https://doi.org/10.1002/rem.21574
institution	INTI International University
building	INTI Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	INTI International University
content_source	INTI Institutional Repository
url_provider	http://eprints.intimal.edu.my
topic	HB Economic Theory HF Commerce
spellingShingle	HB Economic Theory HF Commerce Kok, Kee W Hamzah, A Comparative degradation between heavy and light crude oil mediated by nitrogen‐induced cell‐surface hydrophobicity
description	A bacterial strain UKMP‐10M2 isolated from a Malaysian petroleum refinery was able to degrade 84% of heavy Khafji sour crude and 68% of light Tapis sweet crude within seven days. Analysis of gas chromatography‐flame ionization detector chromatograms show the strain UKMP‐10M2 degraded up to 90% pristane and 50% phytane in heavy crude, but significantly lower pristane (50%) and phytane (30%) were degraded from the light crude. A mixture of aliphatic hexadecane and three‐ring phenanthrene better supported the growth of isolate UKMP‐10M2 compared to using phenanthrene alone, suggesting cometabolism influenced how crude oil with different individual hydrocarbon contents affected the degradation. Peptone as the source of nitrogen increases the emulsifying index in UKMP‐10M2 exposed to heavy Khafji sour crude 20% higher than in light Tapis sweet crude. However, BATH assay showed the same nitrogen source increases bacterial cell surface hydrophobicity of UKMP‐10M2 up to 14% higher in light Tapis crude oil compared to heavy Khafji. This study suggest the nitrogen source plays a decisive role in elevating UKMP‐10M2 bacterial cells hydrophobicity, and in correlation with types of crude oil. Phylogenetic tree analysis based on 16S rDNA sequence results identified the strain to be Rhodococcus ruber.
format	Article
author	Kok, Kee W Hamzah, A
author_facet	Kok, Kee W Hamzah, A
author_sort	Kok, Kee W
title	Comparative degradation between heavy and light crude oil mediated by nitrogen‐induced cell‐surface hydrophobicity
title_short	Comparative degradation between heavy and light crude oil mediated by nitrogen‐induced cell‐surface hydrophobicity
title_full	Comparative degradation between heavy and light crude oil mediated by nitrogen‐induced cell‐surface hydrophobicity
title_fullStr	Comparative degradation between heavy and light crude oil mediated by nitrogen‐induced cell‐surface hydrophobicity
title_full_unstemmed	Comparative degradation between heavy and light crude oil mediated by nitrogen‐induced cell‐surface hydrophobicity
title_sort	comparative degradation between heavy and light crude oil mediated by nitrogen‐induced cell‐surface hydrophobicity
publisher	SpringerLink
publishDate	2018
url	http://eprints.intimal.edu.my/1163/ https://doi.org/10.1002/rem.21574
_version_	1681493065061105664
score	13.226497

Comparative degradation between heavy and light crude oil mediated by nitrogen‐induced cell‐surface hydrophobicity

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