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Toxic Effects in Gaster of Rhizoma Coptidis (Huang Lian) Treated Mice using Histology Technique

The study was conducted to examine the effects of 65 mg/kg, 250 mg/kg and 500 mg/kg of Rhizoma coptidis (Huang Lian) on the changes in gastric epithelium tissue, submucosa tissue and muscularis tissue of mice. In the experiment, sample of gaster organ of control group mice, 65 mg/kg Rhizoma coptidis...

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Main Authors: Nurul Syameera, Aduka, Ai, Wen Lai
Format: Article
Language:English
Published: INTI International University 2018
Subjects:
R Medicine (General)
Online Access:http://eprints.intimal.edu.my/1139/1/v1_2018_7.pdf
http://eprints.intimal.edu.my/1139/
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spelling my-inti-eprints.11392019-10-17T02:27:54Z http://eprints.intimal.edu.my/1139/ Toxic Effects in Gaster of Rhizoma Coptidis (Huang Lian) Treated Mice using Histology Technique Nurul Syameera, Aduka Ai, Wen Lai R Medicine (General) The study was conducted to examine the effects of 65 mg/kg, 250 mg/kg and 500 mg/kg of Rhizoma coptidis (Huang Lian) on the changes in gastric epithelium tissue, submucosa tissue and muscularis tissue of mice. In the experiment, sample of gaster organ of control group mice, 65 mg/kg Rhizoma coptidis treated mice, 250 mg/kg Rhizoma coptidis treated mice and 500 mg/kg Rhizoma coptidis treated mice are collected. All samples are stored in 10% (v/v) neutral buffered formalin solution with pH 6.8-7.2 at 25˚C. In the first procedure, each sample underwent tissue processing and tissue sectioning. Then, data were collected by observing tissues under microscope for gross and microscopic examination. There was no significant change in gross appearance of gaster of control group, 65 mg/kg, 250 mg/kg and 500 mg/kg of Rhizoma coptidis treated mice. In additional, the microscopic examination in the gaster of 65 mg/kg and 250 mg/kg of Rhizoma coptidis treated mice, the layer of mucosa, submucosa and muscularis externa can be identified. Gastric pits can be identified clearly with no sign of erosion. However in the gaster of 500 mg/kg of Rhizoma coptidis treated mice, there were presence of black dots which indicates infiltration of inflammatory cells filling in lamina propria. There was high infiltration rate in mucosa layer indicates inflammation happens at the mucosa layer. Minor detachment of tissue in this 500 mg/kg treated mice can also be observed under microscope. It can be concluded that the gaster of 500 mg/kg of Rhizoma coptidis treated mice brings morphological changes to the tissue cells in gaster, specifically causes infiltration of inflammatory cells, and indicates that 500 mg/kg of R. coptidis carries toxic to the tissue cells at microscopic level. INTI International University 2018-07-01 Article PeerReviewed text en http://eprints.intimal.edu.my/1139/1/v1_2018_7.pdf Nurul Syameera, Aduka and Ai, Wen Lai (2018) Toxic Effects in Gaster of Rhizoma Coptidis (Huang Lian) Treated Mice using Histology Technique. INTI JOURNAL, 1 (7). ISSN e2600-7920
institution INTI International University
building INTI Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider INTI International University
content_source INTI Institutional Repository
url_provider http://eprints.intimal.edu.my
language English
topic R Medicine (General)
spellingShingle R Medicine (General)
Nurul Syameera, Aduka
Ai, Wen Lai
Toxic Effects in Gaster of Rhizoma Coptidis (Huang Lian) Treated Mice using Histology Technique
description The study was conducted to examine the effects of 65 mg/kg, 250 mg/kg and 500 mg/kg of Rhizoma coptidis (Huang Lian) on the changes in gastric epithelium tissue, submucosa tissue and muscularis tissue of mice. In the experiment, sample of gaster organ of control group mice, 65 mg/kg Rhizoma coptidis treated mice, 250 mg/kg Rhizoma coptidis treated mice and 500 mg/kg Rhizoma coptidis treated mice are collected. All samples are stored in 10% (v/v) neutral buffered formalin solution with pH 6.8-7.2 at 25˚C. In the first procedure, each sample underwent tissue processing and tissue sectioning. Then, data were collected by observing tissues under microscope for gross and microscopic examination. There was no significant change in gross appearance of gaster of control group, 65 mg/kg, 250 mg/kg and 500 mg/kg of Rhizoma coptidis treated mice. In additional, the microscopic examination in the gaster of 65 mg/kg and 250 mg/kg of Rhizoma coptidis treated mice, the layer of mucosa, submucosa and muscularis externa can be identified. Gastric pits can be identified clearly with no sign of erosion. However in the gaster of 500 mg/kg of Rhizoma coptidis treated mice, there were presence of black dots which indicates infiltration of inflammatory cells filling in lamina propria. There was high infiltration rate in mucosa layer indicates inflammation happens at the mucosa layer. Minor detachment of tissue in this 500 mg/kg treated mice can also be observed under microscope. It can be concluded that the gaster of 500 mg/kg of Rhizoma coptidis treated mice brings morphological changes to the tissue cells in gaster, specifically causes infiltration of inflammatory cells, and indicates that 500 mg/kg of R. coptidis carries toxic to the tissue cells at microscopic level.
format Article
author Nurul Syameera, Aduka
Ai, Wen Lai
author_facet Nurul Syameera, Aduka
Ai, Wen Lai
author_sort Nurul Syameera, Aduka
title Toxic Effects in Gaster of Rhizoma Coptidis (Huang Lian) Treated Mice using Histology Technique
title_short Toxic Effects in Gaster of Rhizoma Coptidis (Huang Lian) Treated Mice using Histology Technique
title_full Toxic Effects in Gaster of Rhizoma Coptidis (Huang Lian) Treated Mice using Histology Technique
title_fullStr Toxic Effects in Gaster of Rhizoma Coptidis (Huang Lian) Treated Mice using Histology Technique
title_full_unstemmed Toxic Effects in Gaster of Rhizoma Coptidis (Huang Lian) Treated Mice using Histology Technique
title_sort toxic effects in gaster of rhizoma coptidis (huang lian) treated mice using histology technique
publisher INTI International University
publishDate 2018
url http://eprints.intimal.edu.my/1139/1/v1_2018_7.pdf
http://eprints.intimal.edu.my/1139/
_version_ 1651872652148604928
score 13.211869

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