A New Method for Heavy Metals and Aluminium Detection using Biopolymer-based Optical Biosensor
A biopolymer-based biosensor for heavy metals and aluminium (Al) detection was constructed with naturally occurring β-carotene in palm kernel oil used as the biological reporter. The biosensor was designed with β-carotene entrapped by polyurethane- a polymer formed through prepolymerization of...
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Main Authors: | , |
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Format: | Article |
Language: | English |
Published: |
IEEE
2015
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Subjects: | |
Online Access: | http://eprints.intimal.edu.my/11/1/A%20New%20Method%20for%20Heavy%20Metals%20and%20Aluminium%20Detection%20using%20Biopolymer-based%20Optical%20Biosensor.pdf http://eprints.intimal.edu.my/11/ |
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Summary: | A biopolymer-based biosensor for heavy metals and
aluminium (Al) detection was constructed with naturally
occurring β-carotene in palm kernel oil used as the biological
reporter. The biosensor was designed with β-carotene entrapped
by polyurethane- a polymer formed through prepolymerization
of palm kernel oil. The presence of copper (Cu), lead (Pb), zinc
(Zn), and Al was detected through the emulsification of β-
carotene, which caused the change of optical density (OD) at λ =
450 nm. The results showed the OD increased with the presence
of heavy metals and Al within 0.1 mg/L – 10.0 mg/L. The
biosensor was constructed without extra steps to immobilize the
biological component and it was simple to use with one step
detection. Together with high reproducibility and fast response to
Cu within 15 minutes, the biosensor showed good potential to be
developed as a method to detect the presence of heavy metals and
Al. |
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