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Evaluation of Biosurfactant Toxicity Using Phytotoxicity Assay

The inoculum of Pseudomonas sp. was revived from stock culture in glycerol and inoculate into containing minimal salt BH media supplemented with 1% (v/v) n-hexadecane as sole carbon source. The culture was incubated for 7 days to produce the biosurfactants. The calculated biomass of biosurfactant pr...

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Main Author: Tong, Foong Sheng
Format: Thesis
Language:English
Published: 2017
Subjects:
TP Chemical technology
Online Access:http://eprints.intimal.edu.my/1031/1/BBTEI%20146.pdf
http://eprints.intimal.edu.my/1031/
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spelling my-inti-eprints.10312018-06-27T01:16:23Z http://eprints.intimal.edu.my/1031/ Evaluation of Biosurfactant Toxicity Using Phytotoxicity Assay Tong, Foong Sheng TP Chemical technology The inoculum of Pseudomonas sp. was revived from stock culture in glycerol and inoculate into containing minimal salt BH media supplemented with 1% (v/v) n-hexadecane as sole carbon source. The culture was incubated for 7 days to produce the biosurfactants. The calculated biomass of biosurfactant production per gram (dried weight) of bacterial cells were 15.3 g biosurfactant/g cell biomass. The biosurfactants produced was characterized as glycolipid since positive result was obtained in sugar assay and negative result was obtained in protein assay. The ability of biosurfactant to emulsify the engine oil was found equally strong (p<0.05) as the commercial chemical surfactant, Triton X. Seeds of mung beans and fenugreeks were exposed to 0.6 g/L of biosurfactant to assess the phytotoxicity of biosurfactants. The length of roots and shoots of mung bean and fenugreek exposed to biosurfactant showed no significant differences when compared to seeds exposed to deionized water (negative control), but were 6-8 times longer (p<0.05) compared to roots and shoots exposed to Triton X (positive control). The germination index value of mung bean and fenugreek exposed to biosurfactant was 6 and 8 times respectively higher than seeds exposed to Triton X. Hence, the biosurfactant was found to be non-toxic and exhibit no inhibitory effect on the growth of mung bean and fenugreek. Mung bean was more sensitive to biosurfactant when compared to fenugreek based on the GI value obtained. Mung bean could be a better agent for evaluating the toxicity of other biosurfactant. 2017-05-31 Thesis NonPeerReviewed text en http://eprints.intimal.edu.my/1031/1/BBTEI%20146.pdf Tong, Foong Sheng (2017) Evaluation of Biosurfactant Toxicity Using Phytotoxicity Assay. Other thesis, INTI INTERNATIONAL UNIVERSITY.
institution INTI International University
building INTI Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider INTI International University
content_source INTI Institutional Repository
url_provider http://eprints.intimal.edu.my
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Tong, Foong Sheng
Evaluation of Biosurfactant Toxicity Using Phytotoxicity Assay
description The inoculum of Pseudomonas sp. was revived from stock culture in glycerol and inoculate into containing minimal salt BH media supplemented with 1% (v/v) n-hexadecane as sole carbon source. The culture was incubated for 7 days to produce the biosurfactants. The calculated biomass of biosurfactant production per gram (dried weight) of bacterial cells were 15.3 g biosurfactant/g cell biomass. The biosurfactants produced was characterized as glycolipid since positive result was obtained in sugar assay and negative result was obtained in protein assay. The ability of biosurfactant to emulsify the engine oil was found equally strong (p<0.05) as the commercial chemical surfactant, Triton X. Seeds of mung beans and fenugreeks were exposed to 0.6 g/L of biosurfactant to assess the phytotoxicity of biosurfactants. The length of roots and shoots of mung bean and fenugreek exposed to biosurfactant showed no significant differences when compared to seeds exposed to deionized water (negative control), but were 6-8 times longer (p<0.05) compared to roots and shoots exposed to Triton X (positive control). The germination index value of mung bean and fenugreek exposed to biosurfactant was 6 and 8 times respectively higher than seeds exposed to Triton X. Hence, the biosurfactant was found to be non-toxic and exhibit no inhibitory effect on the growth of mung bean and fenugreek. Mung bean was more sensitive to biosurfactant when compared to fenugreek based on the GI value obtained. Mung bean could be a better agent for evaluating the toxicity of other biosurfactant.
format Thesis
author Tong, Foong Sheng
author_facet Tong, Foong Sheng
author_sort Tong, Foong Sheng
title Evaluation of Biosurfactant Toxicity Using Phytotoxicity Assay
title_short Evaluation of Biosurfactant Toxicity Using Phytotoxicity Assay
title_full Evaluation of Biosurfactant Toxicity Using Phytotoxicity Assay
title_fullStr Evaluation of Biosurfactant Toxicity Using Phytotoxicity Assay
title_full_unstemmed Evaluation of Biosurfactant Toxicity Using Phytotoxicity Assay
title_sort evaluation of biosurfactant toxicity using phytotoxicity assay
publishDate 2017
url http://eprints.intimal.edu.my/1031/1/BBTEI%20146.pdf
http://eprints.intimal.edu.my/1031/
_version_ 1644541368076337152
score 13.211869

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