Cover Image

The Combinatorial Effect of Azadirachta indica INEEM) Plant with Amikacin and Tetracycline Against Clinically Important Bacteria

Emergency and rapid spread of antibiotic resistance is due to the overuse and incorrect prescription of antibiotics. Antibiotics resistance has brought negative impacts such as difficulty in treating common infections in mortality rate and prolonged suffering. Current treatments to treat disease c...

Full description

Saved in:
Bibliographic Details
Main Author: Ku, Tze Sin
Format: Thesis
Language:English
Published: 2017
Subjects:
TP Chemical technology
Online Access:http://eprints.intimal.edu.my/1023/6/BBTEI%20140.pdf
http://eprints.intimal.edu.my/1023/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my-inti-eprints.1023
record_format eprints
spelling my-inti-eprints.10232018-06-27T01:43:35Z http://eprints.intimal.edu.my/1023/ The Combinatorial Effect of Azadirachta indica INEEM) Plant with Amikacin and Tetracycline Against Clinically Important Bacteria Ku, Tze Sin TP Chemical technology Emergency and rapid spread of antibiotic resistance is due to the overuse and incorrect prescription of antibiotics. Antibiotics resistance has brought negative impacts such as difficulty in treating common infections in mortality rate and prolonged suffering. Current treatments to treat disease caused by antibiotics-resistant bacteria have become increasingly limited. Therefore, alternative approaches such as the use of plant extracts and combines therapy have been studied to improve the effectiveness of treatment. Plant extracts such as Azadirachta indica (neem) has shown promising use as an antimicrobial agent because it produces various secondary metabolites such as alkaloids and flavonoids. In this study, the combinatorial effect of neem leaf extracts and antibiotics against clinically important pathogens was investigated. The checkboard assay was carried out to determine the minimum inhibitory concentration (MIC) of the neem leaf extract and amikacin as well as tetracycline. However, no MIC could be determined due to the technical error of the microplate reader. Agar well and disc diffusion assay was tetracycline. An antagonistic effect was observed when Bacillus subtilis was exposed to the neem leaf extract and amikacin as a significant reduction (p<0.05) of zone of inhibition from the neem leaf extract and amikacin showed no synergistic effect against Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pneumoniae and Pseudomonas aeruginosa as the diameter of zone of inhibition obtained was the same as that of amikacin alone. No zone of inhibition was observed with Serratia marcescens when tested with neem left extract and amikacin combination. On the other hand, synergism between neem left extract and tetracycline against Propionibacterium acnes, Bacillus subtilis and Streptococcus pneumoniae ere obtained which showed significant enlargement (p<0.0.5) oo zone of inhibition as compared to tht of tetracycline alone. The combinatorial effect of neem left extract and tetracycline alone. No zone of inhibition was observed with Pseudomonas aeruginosa and Serratia marcescens when tested with neem left extract and tetracycline combination. In conclusion, neem left extract showed a better combinatorial effect with tetracycline compared to amikacin due to the synergistic action between neem left extract and tetracycline. Antagonism between neem left extract and tetracycline. Antagonism between neem left extract and amikacin suggest that combining these agents will reduce the efficacy of amikacin 2017 Thesis NonPeerReviewed text en http://eprints.intimal.edu.my/1023/6/BBTEI%20140.pdf Ku, Tze Sin (2017) The Combinatorial Effect of Azadirachta indica INEEM) Plant with Amikacin and Tetracycline Against Clinically Important Bacteria. Other thesis, INTI INTERNATIONAL UNIVERSITY.
institution INTI International University
building INTI Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider INTI International University
content_source INTI Institutional Repository
url_provider http://eprints.intimal.edu.my
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Ku, Tze Sin
The Combinatorial Effect of Azadirachta indica INEEM) Plant with Amikacin and Tetracycline Against Clinically Important Bacteria
description Emergency and rapid spread of antibiotic resistance is due to the overuse and incorrect prescription of antibiotics. Antibiotics resistance has brought negative impacts such as difficulty in treating common infections in mortality rate and prolonged suffering. Current treatments to treat disease caused by antibiotics-resistant bacteria have become increasingly limited. Therefore, alternative approaches such as the use of plant extracts and combines therapy have been studied to improve the effectiveness of treatment. Plant extracts such as Azadirachta indica (neem) has shown promising use as an antimicrobial agent because it produces various secondary metabolites such as alkaloids and flavonoids. In this study, the combinatorial effect of neem leaf extracts and antibiotics against clinically important pathogens was investigated. The checkboard assay was carried out to determine the minimum inhibitory concentration (MIC) of the neem leaf extract and amikacin as well as tetracycline. However, no MIC could be determined due to the technical error of the microplate reader. Agar well and disc diffusion assay was tetracycline. An antagonistic effect was observed when Bacillus subtilis was exposed to the neem leaf extract and amikacin as a significant reduction (p<0.05) of zone of inhibition from the neem leaf extract and amikacin showed no synergistic effect against Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pneumoniae and Pseudomonas aeruginosa as the diameter of zone of inhibition obtained was the same as that of amikacin alone. No zone of inhibition was observed with Serratia marcescens when tested with neem left extract and amikacin combination. On the other hand, synergism between neem left extract and tetracycline against Propionibacterium acnes, Bacillus subtilis and Streptococcus pneumoniae ere obtained which showed significant enlargement (p<0.0.5) oo zone of inhibition as compared to tht of tetracycline alone. The combinatorial effect of neem left extract and tetracycline alone. No zone of inhibition was observed with Pseudomonas aeruginosa and Serratia marcescens when tested with neem left extract and tetracycline combination. In conclusion, neem left extract showed a better combinatorial effect with tetracycline compared to amikacin due to the synergistic action between neem left extract and tetracycline. Antagonism between neem left extract and tetracycline. Antagonism between neem left extract and amikacin suggest that combining these agents will reduce the efficacy of amikacin
format Thesis
author Ku, Tze Sin
author_facet Ku, Tze Sin
author_sort Ku, Tze Sin
title The Combinatorial Effect of Azadirachta indica INEEM) Plant with Amikacin and Tetracycline Against Clinically Important Bacteria
title_short The Combinatorial Effect of Azadirachta indica INEEM) Plant with Amikacin and Tetracycline Against Clinically Important Bacteria
title_full The Combinatorial Effect of Azadirachta indica INEEM) Plant with Amikacin and Tetracycline Against Clinically Important Bacteria
title_fullStr The Combinatorial Effect of Azadirachta indica INEEM) Plant with Amikacin and Tetracycline Against Clinically Important Bacteria
title_full_unstemmed The Combinatorial Effect of Azadirachta indica INEEM) Plant with Amikacin and Tetracycline Against Clinically Important Bacteria
title_sort combinatorial effect of azadirachta indica ineem) plant with amikacin and tetracycline against clinically important bacteria
publishDate 2017
url http://eprints.intimal.edu.my/1023/6/BBTEI%20140.pdf
http://eprints.intimal.edu.my/1023/
_version_ 1644541366017982464
score 13.211869

Similar Items