Investigation of rhizobial dehalogenase control system in dehalogenase gene expression
The DNA sequence upstream of dehE gene encoding dehalogenase E (DehE) of Rhizobium sp. RC1 was determined and contained an open reading frame, designated dehR, which encoded a protein with a significant similarity to dehalogenase regulatory protein (DehR). Plasmid DNA designated pFH648 that carry bo...
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| Format: | Monograph |
| Language: | en |
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Faculty of Sciences
2009
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| Online Access: | http://eprints.utm.my/9755/1/78181.pdf http://eprints.utm.my/9755/ |
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| author | Huyop, Fahrul Zaman Abdul Manan, Fazilah Hussin, Huszalina |
| author_facet | Huyop, Fahrul Zaman Abdul Manan, Fazilah Hussin, Huszalina |
| author_sort | Huyop, Fahrul Zaman |
| building | UTM Library |
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| content_provider | Universiti Teknologi Malaysia |
| content_source | UTM Institutional Repository |
| continent | Asia |
| country | Malaysia |
| description | The DNA sequence upstream of dehE gene encoding dehalogenase E (DehE) of Rhizobium sp. RC1 was determined and contained an open reading frame, designated dehR, which encoded a protein with a significant similarity to dehalogenase regulatory protein (DehR). Plasmid DNA designated pFH648 that carry both dehE and dehR genes were cloned from Rhizobium sp. RC1 genomic DNA. Rhizobium sp. RC1 genetic organization was determined, suggesting dehE was controlled by the product of dehR. Current study proved previous analysis by growth experiment, E.coli XL10 Gold::pFH648 (dehE+, dehR+) has the ability to grow in minimal media supplied with 20 mM D,L-2CP as sole source of carbon. Whereas, E.coli XL10::pSC520 (dehE+) lacking dehR gene and E.coli XL10 Gold::pFH45 (dehR+) lacking dehE gene did not grow in minimal media supplied with 20 mM D,L-2CP as sole source of carbon and energy, suggesting both dehE and dehR genes were needed to allow growth in D,L-2CP minimal media. Since both dehE and dehR were neighboring genes with opposite direction of transcription, promoters of dehE, “24 (TGGCA)/“12 (TTGCTA) and dehR, -10 (ACCA)/-35 (AGGT) were identified by sequence homology. Gel shift experiment supported the proposed genetic organisation and regulation for the Rhizobium sp. RC1 dehalogenase gene. |
| format | Monograph |
| id | my.utm.eprints-9755 |
| institution | Universiti Teknologi Malaysia |
| language | en |
| publishDate | 2009 |
| publisher | Faculty of Sciences |
| record_format | eprints |
| spelling | my.utm.eprints-97552017-08-15T03:43:00Z http://eprints.utm.my/9755/ Investigation of rhizobial dehalogenase control system in dehalogenase gene expression Huyop, Fahrul Zaman Abdul Manan, Fazilah Hussin, Huszalina QA Mathematics The DNA sequence upstream of dehE gene encoding dehalogenase E (DehE) of Rhizobium sp. RC1 was determined and contained an open reading frame, designated dehR, which encoded a protein with a significant similarity to dehalogenase regulatory protein (DehR). Plasmid DNA designated pFH648 that carry both dehE and dehR genes were cloned from Rhizobium sp. RC1 genomic DNA. Rhizobium sp. RC1 genetic organization was determined, suggesting dehE was controlled by the product of dehR. Current study proved previous analysis by growth experiment, E.coli XL10 Gold::pFH648 (dehE+, dehR+) has the ability to grow in minimal media supplied with 20 mM D,L-2CP as sole source of carbon. Whereas, E.coli XL10::pSC520 (dehE+) lacking dehR gene and E.coli XL10 Gold::pFH45 (dehR+) lacking dehE gene did not grow in minimal media supplied with 20 mM D,L-2CP as sole source of carbon and energy, suggesting both dehE and dehR genes were needed to allow growth in D,L-2CP minimal media. Since both dehE and dehR were neighboring genes with opposite direction of transcription, promoters of dehE, “24 (TGGCA)/“12 (TTGCTA) and dehR, -10 (ACCA)/-35 (AGGT) were identified by sequence homology. Gel shift experiment supported the proposed genetic organisation and regulation for the Rhizobium sp. RC1 dehalogenase gene. Faculty of Sciences 2009-08-31 Monograph NonPeerReviewed application/pdf en http://eprints.utm.my/9755/1/78181.pdf Huyop, Fahrul Zaman and Abdul Manan, Fazilah and Hussin, Huszalina (2009) Investigation of rhizobial dehalogenase control system in dehalogenase gene expression. Project Report. Faculty of Sciences, Skudai, Johor. (Unpublished) |
| spellingShingle | QA Mathematics Huyop, Fahrul Zaman Abdul Manan, Fazilah Hussin, Huszalina Investigation of rhizobial dehalogenase control system in dehalogenase gene expression |
| title | Investigation of rhizobial dehalogenase control system in dehalogenase gene expression |
| title_full | Investigation of rhizobial dehalogenase control system in dehalogenase gene expression |
| title_fullStr | Investigation of rhizobial dehalogenase control system in dehalogenase gene expression |
| title_full_unstemmed | Investigation of rhizobial dehalogenase control system in dehalogenase gene expression |
| title_short | Investigation of rhizobial dehalogenase control system in dehalogenase gene expression |
| title_sort | investigation of rhizobial dehalogenase control system in dehalogenase gene expression |
| topic | QA Mathematics |
| url | http://eprints.utm.my/9755/1/78181.pdf http://eprints.utm.my/9755/ |
| url_provider | http://eprints.utm.my/ |