Development of rapid screening method for low-yielding chitosanase activity using Remazol Brilliant Blue-chitosan as substrate
Chitosanolytic activity was monitored using chitosan impregnated with Remazol Brilliant Blue R dye (RBB). Low-yielding chitosan (1%, w/v) hydrolysis was modelled using Viscozyme® L (50%, w/w), a commercial mixed-glucanase, and was found to perform optimally at pH 4.5 and 50 °C. The chitosanase assay...
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| Format: | Article |
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Elsevier BV
2006
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| Online Access: | http://eprints.utm.my/9084/ http://dx.doi.org/10.1016/j.enzmictec.2005.06.006 |
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| author | Fen, Lee Lih Md.Illias, Rosli Kamaruddin, Kamarulzaman Maskat, Mohd Yusof Hassan, Osman |
| author_facet | Fen, Lee Lih Md.Illias, Rosli Kamaruddin, Kamarulzaman Maskat, Mohd Yusof Hassan, Osman |
| author_sort | Fen, Lee Lih |
| building | UTM Library |
| collection | Institutional Repository |
| content_provider | Universiti Teknologi Malaysia |
| content_source | UTM Institutional Repository |
| continent | Asia |
| country | Malaysia |
| description | Chitosanolytic activity was monitored using chitosan impregnated with Remazol Brilliant Blue R dye (RBB). Low-yielding chitosan (1%, w/v) hydrolysis was modelled using Viscozyme® L (50%, w/w), a commercial mixed-glucanase, and was found to perform optimally at pH 4.5 and 50 °C. The chitosanase assay was performed on powdered (RBB-P) and colloidal form (RBB-C) of the dyed chitosan. Hydrolysis of chitosan was accompanied by the release of water-soluble dyed-hydrolysate, which can be monitored rapidly using microplate reader at 595 nm. The result obtained from this method was in agreement with the determination of reducing end by using the traditional, complex and time-consuming DNS method. The method is significantly rapid and more sensitive than DNS method. |
| format | Article |
| id | my.utm.eprints-9084 |
| institution | Universiti Teknologi Malaysia |
| publishDate | 2006 |
| publisher | Elsevier BV |
| record_format | eprints |
| spelling | my.utm.eprints-90842018-03-07T21:10:41Z http://eprints.utm.my/9084/ Development of rapid screening method for low-yielding chitosanase activity using Remazol Brilliant Blue-chitosan as substrate Fen, Lee Lih Md.Illias, Rosli Kamaruddin, Kamarulzaman Maskat, Mohd Yusof Hassan, Osman TP Chemical technology Chitosanolytic activity was monitored using chitosan impregnated with Remazol Brilliant Blue R dye (RBB). Low-yielding chitosan (1%, w/v) hydrolysis was modelled using Viscozyme® L (50%, w/w), a commercial mixed-glucanase, and was found to perform optimally at pH 4.5 and 50 °C. The chitosanase assay was performed on powdered (RBB-P) and colloidal form (RBB-C) of the dyed chitosan. Hydrolysis of chitosan was accompanied by the release of water-soluble dyed-hydrolysate, which can be monitored rapidly using microplate reader at 595 nm. The result obtained from this method was in agreement with the determination of reducing end by using the traditional, complex and time-consuming DNS method. The method is significantly rapid and more sensitive than DNS method. Elsevier BV 2006 Article PeerReviewed Fen, Lee Lih and Md.Illias, Rosli and Kamaruddin, Kamarulzaman and Maskat, Mohd Yusof and Hassan, Osman (2006) Development of rapid screening method for low-yielding chitosanase activity using Remazol Brilliant Blue-chitosan as substrate. Enzyme and Microbial Technology, 38 (1-2). pp. 215-219. ISSN Enzyme and Microbial Technology http://dx.doi.org/10.1016/j.enzmictec.2005.06.006 10.1016/j.enzmictec.2005.06.006 |
| spellingShingle | TP Chemical technology Fen, Lee Lih Md.Illias, Rosli Kamaruddin, Kamarulzaman Maskat, Mohd Yusof Hassan, Osman Development of rapid screening method for low-yielding chitosanase activity using Remazol Brilliant Blue-chitosan as substrate |
| title | Development of rapid screening method for low-yielding chitosanase activity using Remazol Brilliant Blue-chitosan as substrate |
| title_full | Development of rapid screening method for low-yielding chitosanase activity using Remazol Brilliant Blue-chitosan as substrate |
| title_fullStr | Development of rapid screening method for low-yielding chitosanase activity using Remazol Brilliant Blue-chitosan as substrate |
| title_full_unstemmed | Development of rapid screening method for low-yielding chitosanase activity using Remazol Brilliant Blue-chitosan as substrate |
| title_short | Development of rapid screening method for low-yielding chitosanase activity using Remazol Brilliant Blue-chitosan as substrate |
| title_sort | development of rapid screening method for low-yielding chitosanase activity using remazol brilliant blue-chitosan as substrate |
| topic | TP Chemical technology |
| url | http://eprints.utm.my/9084/ http://dx.doi.org/10.1016/j.enzmictec.2005.06.006 |
| url_provider | http://eprints.utm.my/ |