Enzymatic study of the Dehalogenases involved in biodegradation of a herbicide 3-chloropropionic acid (3cp) by a novel indigenous bacteria
3-chloropropionic acid is synthetic halogenated compounds used in herbicide. A bacterium isolated from a soil sample and characterised as Rhodococcus sp. by 16S rRNA analysis, was able to degrade and utilised 3-chloropropionate as the sole source of carbon and energy. This was supported by the abili...
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| Format: | Monograph |
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Faculty of Biosciences and Bioengineering
2008
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| Online Access: | http://eprints.utm.my/9065/ |
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| author | Huyop, Fahrul Zaman Hamzah, Ainon Dayang Fredalina, Basri Jaafar, Jafariah Raja Abd Rahman, Raja Noor Zaliha |
| author_facet | Huyop, Fahrul Zaman Hamzah, Ainon Dayang Fredalina, Basri Jaafar, Jafariah Raja Abd Rahman, Raja Noor Zaliha |
| author_sort | Huyop, Fahrul Zaman |
| building | UTM Library |
| collection | Institutional Repository |
| content_provider | Universiti Teknologi Malaysia |
| content_source | UTM Institutional Repository |
| continent | Asia |
| country | Malaysia |
| description | 3-chloropropionic acid is synthetic halogenated compounds used in herbicide. A bacterium isolated from a soil sample and characterised as Rhodococcus sp. by 16S rRNA analysis, was able to degrade and utilised 3-chloropropionate as the sole source of carbon and energy. This was supported by the ability of the bacterium to grow on 20 mM 3-chloropropionate with a doubling time of 11.72 hours. The utilisation of 3-chloropropionate was also confirmed by detection of 3-chloropropionate depletion in the medium using HPLC (High Performance Liquid Chromatography). Cell free extract of Rhodococcus sp. had an enzyme specific activity of 0.013 μmol Cl-/min/mg protein towards 3-chloropropionate. |
| format | Monograph |
| id | my.utm.eprints-9065 |
| institution | Universiti Teknologi Malaysia |
| publishDate | 2008 |
| publisher | Faculty of Biosciences and Bioengineering |
| record_format | eprints |
| spelling | my.utm.eprints-90652017-08-14T06:45:56Z http://eprints.utm.my/9065/ Enzymatic study of the Dehalogenases involved in biodegradation of a herbicide 3-chloropropionic acid (3cp) by a novel indigenous bacteria Huyop, Fahrul Zaman Hamzah, Ainon Dayang Fredalina, Basri Jaafar, Jafariah Raja Abd Rahman, Raja Noor Zaliha 3-chloropropionic acid is synthetic halogenated compounds used in herbicide. A bacterium isolated from a soil sample and characterised as Rhodococcus sp. by 16S rRNA analysis, was able to degrade and utilised 3-chloropropionate as the sole source of carbon and energy. This was supported by the ability of the bacterium to grow on 20 mM 3-chloropropionate with a doubling time of 11.72 hours. The utilisation of 3-chloropropionate was also confirmed by detection of 3-chloropropionate depletion in the medium using HPLC (High Performance Liquid Chromatography). Cell free extract of Rhodococcus sp. had an enzyme specific activity of 0.013 μmol Cl-/min/mg protein towards 3-chloropropionate. Faculty of Biosciences and Bioengineering 2008-11-30 Monograph NonPeerReviewed Huyop, Fahrul Zaman and Hamzah, Ainon and Dayang Fredalina, Basri and Jaafar, Jafariah and Raja Abd Rahman, Raja Noor Zaliha (2008) Enzymatic study of the Dehalogenases involved in biodegradation of a herbicide 3-chloropropionic acid (3cp) by a novel indigenous bacteria. Project Report. Faculty of Biosciences and Bioengineering, Skudai, Johor. (Unpublished) |
| spellingShingle | Huyop, Fahrul Zaman Hamzah, Ainon Dayang Fredalina, Basri Jaafar, Jafariah Raja Abd Rahman, Raja Noor Zaliha Enzymatic study of the Dehalogenases involved in biodegradation of a herbicide 3-chloropropionic acid (3cp) by a novel indigenous bacteria |
| title | Enzymatic study of the Dehalogenases involved in biodegradation of a herbicide 3-chloropropionic acid (3cp) by a novel indigenous bacteria |
| title_full | Enzymatic study of the Dehalogenases involved in biodegradation of a herbicide 3-chloropropionic acid (3cp) by a novel indigenous bacteria |
| title_fullStr | Enzymatic study of the Dehalogenases involved in biodegradation of a herbicide 3-chloropropionic acid (3cp) by a novel indigenous bacteria |
| title_full_unstemmed | Enzymatic study of the Dehalogenases involved in biodegradation of a herbicide 3-chloropropionic acid (3cp) by a novel indigenous bacteria |
| title_short | Enzymatic study of the Dehalogenases involved in biodegradation of a herbicide 3-chloropropionic acid (3cp) by a novel indigenous bacteria |
| title_sort | enzymatic study of the dehalogenases involved in biodegradation of a herbicide 3-chloropropionic acid (3cp) by a novel indigenous bacteria |
| url | http://eprints.utm.my/9065/ |
| url_provider | http://eprints.utm.my/ |