Separation of tocol-derivatives by elevated temperature normal-phase liquid chromatography
A novel method for the separation of eight vitamin E isomers (-, -, -, -tocopherol, and -, -, -,  tocotrienol) and -tocopherol acetate on normal phase high performance liquid chromatography (NP-HPLC) at elevated column temperature has been developed. The separation was opt...
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| Language: | en |
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Penerbit UTM Press
2006
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| Online Access: | http://eprints.utm.my/7942/1/JTDIS45C%5BC%5DMarsin.pdf http://eprints.utm.my/7942/ http://www.penerbit.utm.my/onlinejournal/45/C/JTDIS45C3.pdf |
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| author | Sanagi, Mohd Marsin See, Hong Heng Wan Ibrahim, Wan Aini Abu Naim, Ahmedy |
| author_facet | Sanagi, Mohd Marsin See, Hong Heng Wan Ibrahim, Wan Aini Abu Naim, Ahmedy |
| author_sort | Sanagi, Mohd Marsin |
| building | UTM Library |
| collection | Institutional Repository |
| content_provider | Universiti Teknologi Malaysia |
| content_source | UTM Institutional Repository |
| continent | Asia |
| country | Malaysia |
| description | A novel method for the separation of eight vitamin E isomers (-, -, -, -tocopherol, and -, -, -,  tocotrienol) and -tocopherol acetate on normal phase high performance liquid chromatography (NP-HPLC) at elevated column temperature has been developed. The separation was optimized by varying the eluent composition and column temperature. All the isomers were successfully separated using NP-HPLC on amino and silica columns. By simply increasing the temperature for silica column, excellent separation efficiencies and shorter analysis times were achieved without significant loss in resolution. The developed separation method is rapid, shows excellent reproducibility, and suitable to be used as a quantitative method in analyzing tocopherols and tocotrienols. |
| format | Article |
| id | my.utm.eprints-7942 |
| institution | Universiti Teknologi Malaysia |
| language | en |
| publishDate | 2006 |
| publisher | Penerbit UTM Press |
| record_format | eprints |
| spelling | my.utm.eprints-79422010-10-28T04:56:43Z http://eprints.utm.my/7942/ Separation of tocol-derivatives by elevated temperature normal-phase liquid chromatography Sanagi, Mohd Marsin See, Hong Heng Wan Ibrahim, Wan Aini Abu Naim, Ahmedy QD Chemistry A novel method for the separation of eight vitamin E isomers (-, -, -, -tocopherol, and -, -, -,  tocotrienol) and -tocopherol acetate on normal phase high performance liquid chromatography (NP-HPLC) at elevated column temperature has been developed. The separation was optimized by varying the eluent composition and column temperature. All the isomers were successfully separated using NP-HPLC on amino and silica columns. By simply increasing the temperature for silica column, excellent separation efficiencies and shorter analysis times were achieved without significant loss in resolution. The developed separation method is rapid, shows excellent reproducibility, and suitable to be used as a quantitative method in analyzing tocopherols and tocotrienols. Penerbit UTM Press 2006-12 Article PeerReviewed application/pdf en http://eprints.utm.my/7942/1/JTDIS45C%5BC%5DMarsin.pdf Sanagi, Mohd Marsin and See, Hong Heng and Wan Ibrahim, Wan Aini and Abu Naim, Ahmedy (2006) Separation of tocol-derivatives by elevated temperature normal-phase liquid chromatography. Jurnal Teknologi C (45C). pp. 29-40. ISSN 0127-9696 http://www.penerbit.utm.my/onlinejournal/45/C/JTDIS45C3.pdf |
| spellingShingle | QD Chemistry Sanagi, Mohd Marsin See, Hong Heng Wan Ibrahim, Wan Aini Abu Naim, Ahmedy Separation of tocol-derivatives by elevated temperature normal-phase liquid chromatography |
| title | Separation of tocol-derivatives by elevated temperature normal-phase liquid chromatography |
| title_full | Separation of tocol-derivatives by elevated temperature normal-phase liquid chromatography |
| title_fullStr | Separation of tocol-derivatives by elevated temperature normal-phase liquid chromatography |
| title_full_unstemmed | Separation of tocol-derivatives by elevated temperature normal-phase liquid chromatography |
| title_short | Separation of tocol-derivatives by elevated temperature normal-phase liquid chromatography |
| title_sort | separation of tocol-derivatives by elevated temperature normal-phase liquid chromatography |
| topic | QD Chemistry |
| url | http://eprints.utm.my/7942/1/JTDIS45C%5BC%5DMarsin.pdf http://eprints.utm.my/7942/ http://www.penerbit.utm.my/onlinejournal/45/C/JTDIS45C3.pdf |
| url_provider | http://eprints.utm.my/ |