Production of cyclodextrin glucanotransferase (CGTase) from Bacillus stearothermophilus HR1 in shake flasks
The objective of this paper is to increase CGTase production from Bacillus stearothermophilus HR1 by optimization of medium formulation and fermentation variables. Central composite factorial design was used to achieve this purpose. Interpretation of results was carried out using the analysis of var...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | en |
| Published: |
Universiti Malaysia Sabah
2003
|
| Subjects: | |
| Online Access: | http://eprints.utm.my/7211/1/WongTienEe2003_ProductionOfCyclodextrinGlucanotransferase%28CGTase%29.pdf http://eprints.utm.my/7211/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1845471769183387648 |
|---|---|
| author | Wong, Tien Ee Abd. Rahman, Roshanida Illias, R. M. Md. Salleh, Madihah Hassan, Osman Kamaruddin, Kamarulzaman Nik Mahmood, Nik Azmi |
| author_facet | Wong, Tien Ee Abd. Rahman, Roshanida Illias, R. M. Md. Salleh, Madihah Hassan, Osman Kamaruddin, Kamarulzaman Nik Mahmood, Nik Azmi |
| author_sort | Wong, Tien Ee |
| building | UTM Library |
| collection | Institutional Repository |
| content_provider | Universiti Teknologi Malaysia |
| content_source | UTM Institutional Repository |
| continent | Asia |
| country | Malaysia |
| description | The objective of this paper is to increase CGTase production from Bacillus stearothermophilus HR1 by optimization of medium formulation and fermentation variables. Central composite factorial design was used to achieve this purpose. Interpretation of results was carried out using the analysis of variance (ANOVA) and analyzed using the 3-D response surface plot. The parameters studied in the medium formulation were concentration of sago starch, peptone casein, K2HPO4, CaCl2 and initial pH of the medium. Increase in the enzyme activity up to 14.20 U/ml, with the regression constant (R2) of 0.9486. For optimization of pH and agitation speed, culture was incubated in shake flasks at 55C. CGTase produced was 16.15 U/ml, with a regresssion of 92.34%. An increase of 3.8 fold in CGTase activity
was obtained after optimization. We have successfully optimized the production of CGTase from Bacillus stearothermophilus HR1 using experimental design approach. |
| format | Article |
| id | my.utm.eprints-7211 |
| institution | Universiti Teknologi Malaysia |
| language | en |
| publishDate | 2003 |
| publisher | Universiti Malaysia Sabah |
| record_format | eprints |
| spelling | my.utm.eprints-72112017-10-11T01:27:27Z http://eprints.utm.my/7211/ Production of cyclodextrin glucanotransferase (CGTase) from Bacillus stearothermophilus HR1 in shake flasks Wong, Tien Ee Abd. Rahman, Roshanida Illias, R. M. Md. Salleh, Madihah Hassan, Osman Kamaruddin, Kamarulzaman Nik Mahmood, Nik Azmi T Technology (General) The objective of this paper is to increase CGTase production from Bacillus stearothermophilus HR1 by optimization of medium formulation and fermentation variables. Central composite factorial design was used to achieve this purpose. Interpretation of results was carried out using the analysis of variance (ANOVA) and analyzed using the 3-D response surface plot. The parameters studied in the medium formulation were concentration of sago starch, peptone casein, K2HPO4, CaCl2 and initial pH of the medium. Increase in the enzyme activity up to 14.20 U/ml, with the regression constant (R2) of 0.9486. For optimization of pH and agitation speed, culture was incubated in shake flasks at 55C. CGTase produced was 16.15 U/ml, with a regresssion of 92.34%. An increase of 3.8 fold in CGTase activity was obtained after optimization. We have successfully optimized the production of CGTase from Bacillus stearothermophilus HR1 using experimental design approach. Universiti Malaysia Sabah 2003 Article PeerReviewed application/pdf en http://eprints.utm.my/7211/1/WongTienEe2003_ProductionOfCyclodextrinGlucanotransferase%28CGTase%29.pdf Wong, Tien Ee and Abd. Rahman, Roshanida and Illias, R. M. and Md. Salleh, Madihah and Hassan, Osman and Kamaruddin, Kamarulzaman and Nik Mahmood, Nik Azmi (2003) Production of cyclodextrin glucanotransferase (CGTase) from Bacillus stearothermophilus HR1 in shake flasks. Proceedings of International Conference On Chemical and Bioprocess Engineering . pp. 222-225. |
| spellingShingle | T Technology (General) Wong, Tien Ee Abd. Rahman, Roshanida Illias, R. M. Md. Salleh, Madihah Hassan, Osman Kamaruddin, Kamarulzaman Nik Mahmood, Nik Azmi Production of cyclodextrin glucanotransferase (CGTase) from Bacillus stearothermophilus HR1 in shake flasks |
| title | Production of cyclodextrin glucanotransferase (CGTase) from Bacillus stearothermophilus HR1 in shake flasks |
| title_full | Production of cyclodextrin glucanotransferase (CGTase) from Bacillus stearothermophilus HR1 in shake flasks |
| title_fullStr | Production of cyclodextrin glucanotransferase (CGTase) from Bacillus stearothermophilus HR1 in shake flasks |
| title_full_unstemmed | Production of cyclodextrin glucanotransferase (CGTase) from Bacillus stearothermophilus HR1 in shake flasks |
| title_short | Production of cyclodextrin glucanotransferase (CGTase) from Bacillus stearothermophilus HR1 in shake flasks |
| title_sort | production of cyclodextrin glucanotransferase (cgtase) from bacillus stearothermophilus hr1 in shake flasks |
| topic | T Technology (General) |
| url | http://eprints.utm.my/7211/1/WongTienEe2003_ProductionOfCyclodextrinGlucanotransferase%28CGTase%29.pdf http://eprints.utm.my/7211/ |
| url_provider | http://eprints.utm.my/ |