Expression of the Na+/H+ antiporter gene (g1-nhaC) of alkaliphilic bacillus sp. G1 in escherichia coli
A Na+/H+ antiporter gene was isolated from alkaliphilic Bacillus sp. G1. The full-length sequence of the Na+/H+ antiporter gene was obtained using a genome walking method, and designated as g1-nhaC. An ORF preceded by a promoter-like sequence and a Shine—Dalgarno sequence, and followed by a termin...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | en |
| Published: |
Blackwell Publishing Ltd.
2007
|
| Subjects: | |
| Online Access: | http://eprints.utm.my/5903/1/ChongWaiLiew2007_ExpressionOfTheNa%2BH%2BAntiporterGene.pdf http://eprints.utm.my/5903/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1845471482705084416 |
|---|---|
| author | Chong, Wai Liew Md. Illias, Rosli Mahadi, Nor Muhammad Najimudin, Nazalan |
| author_facet | Chong, Wai Liew Md. Illias, Rosli Mahadi, Nor Muhammad Najimudin, Nazalan |
| author_sort | Chong, Wai Liew |
| building | UTM Library |
| collection | Institutional Repository |
| content_provider | Universiti Teknologi Malaysia |
| content_source | UTM Institutional Repository |
| continent | Asia |
| country | Malaysia |
| description | A Na+/H+ antiporter gene was isolated from alkaliphilic Bacillus sp. G1. The full-length sequence of the Na+/H+ antiporter gene was obtained using a genome walking method, and designated as g1-nhaC. An ORF preceded by a promoter-like sequence and a Shine—Dalgarno sequence, and followed by a terminator-like sequence was identified. The deduced amino acid sequence consists of 535 amino acids, and a calculated molecular mass of 57 776 Da. g1-nhaC was subsequently cloned into pET22b(+) and expressed in Escherichia coli BL21 (DE3). Recombinant E. coli harboring the g1-nhaC gene was able to grow in modified L medium at various concentrations of NaCl (0.2-2.0 M) at different pH values. The recombinant bacteria grew well in the medium with concentrations of NaCl as high as 1.75 M at pH 8.0-9.0. Minimal growth was observed at 2.0 M NaCI, pH 8.0-9.0. At pH 10, the recombinant bacteria grew well in a medium with a low concentration of NaCl (0.2 M). These results suggested that the g1-NhaC antiporter from Bacillus sp. G1 plays a role in Na+ extrusion at lower pH values and in pH homeostasis at pH 10 under Na+ -limiting conditions. |
| format | Article |
| id | my.utm.eprints-5903 |
| institution | Universiti Teknologi Malaysia |
| language | en |
| publishDate | 2007 |
| publisher | Blackwell Publishing Ltd. |
| record_format | eprints |
| spelling | my.utm.eprints-59032010-06-01T15:36:23Z http://eprints.utm.my/5903/ Expression of the Na+/H+ antiporter gene (g1-nhaC) of alkaliphilic bacillus sp. G1 in escherichia coli Chong, Wai Liew Md. Illias, Rosli Mahadi, Nor Muhammad Najimudin, Nazalan T Technology (General) A Na+/H+ antiporter gene was isolated from alkaliphilic Bacillus sp. G1. The full-length sequence of the Na+/H+ antiporter gene was obtained using a genome walking method, and designated as g1-nhaC. An ORF preceded by a promoter-like sequence and a Shine—Dalgarno sequence, and followed by a terminator-like sequence was identified. The deduced amino acid sequence consists of 535 amino acids, and a calculated molecular mass of 57 776 Da. g1-nhaC was subsequently cloned into pET22b(+) and expressed in Escherichia coli BL21 (DE3). Recombinant E. coli harboring the g1-nhaC gene was able to grow in modified L medium at various concentrations of NaCl (0.2-2.0 M) at different pH values. The recombinant bacteria grew well in the medium with concentrations of NaCl as high as 1.75 M at pH 8.0-9.0. Minimal growth was observed at 2.0 M NaCI, pH 8.0-9.0. At pH 10, the recombinant bacteria grew well in a medium with a low concentration of NaCl (0.2 M). These results suggested that the g1-NhaC antiporter from Bacillus sp. G1 plays a role in Na+ extrusion at lower pH values and in pH homeostasis at pH 10 under Na+ -limiting conditions. Blackwell Publishing Ltd. 2007 Article PeerReviewed application/pdf en http://eprints.utm.my/5903/1/ChongWaiLiew2007_ExpressionOfTheNa%2BH%2BAntiporterGene.pdf Chong, Wai Liew and Md. Illias, Rosli and Mahadi, Nor Muhammad and Najimudin, Nazalan (2007) Expression of the Na+/H+ antiporter gene (g1-nhaC) of alkaliphilic bacillus sp. G1 in escherichia coli. FEMS Microbiology Letters, 276 . pp. 114-122. |
| spellingShingle | T Technology (General) Chong, Wai Liew Md. Illias, Rosli Mahadi, Nor Muhammad Najimudin, Nazalan Expression of the Na+/H+ antiporter gene (g1-nhaC) of alkaliphilic bacillus sp. G1 in escherichia coli |
| title | Expression of the Na+/H+ antiporter gene (g1-nhaC) of alkaliphilic bacillus sp. G1 in escherichia coli |
| title_full | Expression of the Na+/H+ antiporter gene (g1-nhaC) of alkaliphilic bacillus sp. G1 in escherichia coli |
| title_fullStr | Expression of the Na+/H+ antiporter gene (g1-nhaC) of alkaliphilic bacillus sp. G1 in escherichia coli |
| title_full_unstemmed | Expression of the Na+/H+ antiporter gene (g1-nhaC) of alkaliphilic bacillus sp. G1 in escherichia coli |
| title_short | Expression of the Na+/H+ antiporter gene (g1-nhaC) of alkaliphilic bacillus sp. G1 in escherichia coli |
| title_sort | expression of the na+/h+ antiporter gene (g1-nhac) of alkaliphilic bacillus sp. g1 in escherichia coli |
| topic | T Technology (General) |
| url | http://eprints.utm.my/5903/1/ChongWaiLiew2007_ExpressionOfTheNa%2BH%2BAntiporterGene.pdf http://eprints.utm.my/5903/ |
| url_provider | http://eprints.utm.my/ |