The effect of different plant growth regulators on shoot induction of orthosiphon aristatus bolding

Among herbal plants which had been commercialized recently include Orthosiphon aristatus Boldingh. or better known as Java Tea and “misai kucing”. The plant’s leaves are believed to be able to help prevent and eliminate bladder stone apart from some other purposes when used alone or in combina...

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Main Authors: Rahmat, Zaidah, Nazri, M. S.
Format: Article
Language:en
Published: 2007
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Online Access:http://eprints.utm.my/3036/1/Seminar_on_Med_and_Aromatic_Plants_zaidah.pdf
http://eprints.utm.my/3036/
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author Rahmat, Zaidah
Nazri, M. S.
author_facet Rahmat, Zaidah
Nazri, M. S.
author_sort Rahmat, Zaidah
building UTM Library
collection Institutional Repository
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
continent Asia
country Malaysia
description Among herbal plants which had been commercialized recently include Orthosiphon aristatus Boldingh. or better known as Java Tea and “misai kucing”. The plant’s leaves are believed to be able to help prevent and eliminate bladder stone apart from some other purposes when used alone or in combination with other plants. The production of the plant using tissue culture technique is hoped to reduce the time for growth under normal cultivation and to produce in a large quantity from a small source of explant. Sources of explant for this study were derived from leaves and stems for comparison. Each of the explants was grown under various plant growth regulators at various concentrations and combinations to determine the best hormone concentration that give adventitious shoots. Explants from stems were found to produce the best overall result with 0.2 mg/L BAP and 0.5 mg/L NAA but at a slower rate. The best result obtained from leaves explants was with 0.2 mg/L BAP which induces shoot faster than stem explants but at a smaller quantity. In comparison to the results from leaves explants, BAP with the same concentration using the stem explants gave a higher number of adventitious shoots. Stems are found to be a better explants source for regeneration of O. aristatus. Keywords: Orthosiphon aristatus, stem, leaves, BAP, NAA, adventitious shoots.
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spelling my.utm.eprints-30362017-10-24T01:02:02Z http://eprints.utm.my/3036/ The effect of different plant growth regulators on shoot induction of orthosiphon aristatus bolding Rahmat, Zaidah Nazri, M. S. QK Botany Among herbal plants which had been commercialized recently include Orthosiphon aristatus Boldingh. or better known as Java Tea and “misai kucing”. The plant’s leaves are believed to be able to help prevent and eliminate bladder stone apart from some other purposes when used alone or in combination with other plants. The production of the plant using tissue culture technique is hoped to reduce the time for growth under normal cultivation and to produce in a large quantity from a small source of explant. Sources of explant for this study were derived from leaves and stems for comparison. Each of the explants was grown under various plant growth regulators at various concentrations and combinations to determine the best hormone concentration that give adventitious shoots. Explants from stems were found to produce the best overall result with 0.2 mg/L BAP and 0.5 mg/L NAA but at a slower rate. The best result obtained from leaves explants was with 0.2 mg/L BAP which induces shoot faster than stem explants but at a smaller quantity. In comparison to the results from leaves explants, BAP with the same concentration using the stem explants gave a higher number of adventitious shoots. Stems are found to be a better explants source for regeneration of O. aristatus. Keywords: Orthosiphon aristatus, stem, leaves, BAP, NAA, adventitious shoots. 2007 Article NonPeerReviewed application/pdf en http://eprints.utm.my/3036/1/Seminar_on_Med_and_Aromatic_Plants_zaidah.pdf Rahmat, Zaidah and Nazri, M. S. (2007) The effect of different plant growth regulators on shoot induction of orthosiphon aristatus bolding. Seminar on Medicinal and Aromatic Plant .
spellingShingle QK Botany
Rahmat, Zaidah
Nazri, M. S.
The effect of different plant growth regulators on shoot induction of orthosiphon aristatus bolding
title The effect of different plant growth regulators on shoot induction of orthosiphon aristatus bolding
title_full The effect of different plant growth regulators on shoot induction of orthosiphon aristatus bolding
title_fullStr The effect of different plant growth regulators on shoot induction of orthosiphon aristatus bolding
title_full_unstemmed The effect of different plant growth regulators on shoot induction of orthosiphon aristatus bolding
title_short The effect of different plant growth regulators on shoot induction of orthosiphon aristatus bolding
title_sort effect of different plant growth regulators on shoot induction of orthosiphon aristatus bolding
topic QK Botany
url http://eprints.utm.my/3036/1/Seminar_on_Med_and_Aromatic_Plants_zaidah.pdf
http://eprints.utm.my/3036/
url_provider http://eprints.utm.my/