Isolation and characterization of adhesive protein from green mussel (perna viridis)
Mussel ejaculate threads from phenol gland which is situated at its leg area. These threads are built from adhesive protein, which contain amino aromatic L-dihydroxyphenylalanine (L-DOPA) acid. The objective of this study is to isolate and characterize adhesive protein from green mussel (perna verid...
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| Format: | Monograph |
| Language: | en |
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Universiti Teknologi Malaysia
2000
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| Online Access: | http://eprints.utm.my/2663/1/71451.pdf http://eprints.utm.my/2663/ |
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| author | Syed Muhammad, Syed Annuar Fauad Md. Illias, Rosli Nik Mahmod, Nik Azmi |
| author_facet | Syed Muhammad, Syed Annuar Fauad Md. Illias, Rosli Nik Mahmod, Nik Azmi |
| author_sort | Syed Muhammad, Syed Annuar Fauad |
| building | UTM Library |
| collection | Institutional Repository |
| content_provider | Universiti Teknologi Malaysia |
| content_source | UTM Institutional Repository |
| continent | Asia |
| country | Malaysia |
| description | Mussel ejaculate threads from phenol gland which is situated at its leg area. These threads are built from adhesive protein, which contain amino aromatic L-dihydroxyphenylalanine (L-DOPA) acid. The objective of this study is to isolate and characterize adhesive protein from green mussel (perna veridis). First stage of the experiment is to isolate the protein from mussel feet by extraction method by using nitrogen liquid and dissolved in 0.7% perchloric acid followed by sulfuric acid and acetone. Second stage is to purify the protein using Sephadex Gel (G-200) and finally the characterization and identification by using HPLC, TLC, and electrophoresis gel. The protein concentrations after purification were measured using spectrophotometer with wave length 260 nm. Results for the three highest OD value samples showed that L-DOPA has been detected in the samples at Rf 0.15, 0.21, and 0.10. Results from HPLC showed that there are two types of amino acids contained in the samples. The first peak represents alanine while the second peak represents L-DOPA. From the samples amount of 6 gram, HPLC can detect the concentration of L-DOPA between 0.05 to 3 mg/L. However, characterization using electrophoresis gel was unsuccessful to determine the weight of protein in the samples. |
| format | Monograph |
| id | my.utm.eprints-2663 |
| institution | Universiti Teknologi Malaysia |
| language | en |
| publishDate | 2000 |
| publisher | Universiti Teknologi Malaysia |
| record_format | eprints |
| spelling | my.utm.eprints-26632010-06-01T03:04:12Z http://eprints.utm.my/2663/ Isolation and characterization of adhesive protein from green mussel (perna viridis) Syed Muhammad, Syed Annuar Fauad Md. Illias, Rosli Nik Mahmod, Nik Azmi TP Chemical technology Mussel ejaculate threads from phenol gland which is situated at its leg area. These threads are built from adhesive protein, which contain amino aromatic L-dihydroxyphenylalanine (L-DOPA) acid. The objective of this study is to isolate and characterize adhesive protein from green mussel (perna veridis). First stage of the experiment is to isolate the protein from mussel feet by extraction method by using nitrogen liquid and dissolved in 0.7% perchloric acid followed by sulfuric acid and acetone. Second stage is to purify the protein using Sephadex Gel (G-200) and finally the characterization and identification by using HPLC, TLC, and electrophoresis gel. The protein concentrations after purification were measured using spectrophotometer with wave length 260 nm. Results for the three highest OD value samples showed that L-DOPA has been detected in the samples at Rf 0.15, 0.21, and 0.10. Results from HPLC showed that there are two types of amino acids contained in the samples. The first peak represents alanine while the second peak represents L-DOPA. From the samples amount of 6 gram, HPLC can detect the concentration of L-DOPA between 0.05 to 3 mg/L. However, characterization using electrophoresis gel was unsuccessful to determine the weight of protein in the samples. Universiti Teknologi Malaysia 2000-12-31 Monograph NonPeerReviewed application/pdf en http://eprints.utm.my/2663/1/71451.pdf Syed Muhammad, Syed Annuar Fauad and Md. Illias, Rosli and Nik Mahmod, Nik Azmi (2000) Isolation and characterization of adhesive protein from green mussel (perna viridis). Project Report. Universiti Teknologi Malaysia. (Unpublished) |
| spellingShingle | TP Chemical technology Syed Muhammad, Syed Annuar Fauad Md. Illias, Rosli Nik Mahmod, Nik Azmi Isolation and characterization of adhesive protein from green mussel (perna viridis) |
| title | Isolation and characterization of adhesive protein from green mussel (perna viridis) |
| title_full | Isolation and characterization of adhesive protein from green mussel (perna viridis) |
| title_fullStr | Isolation and characterization of adhesive protein from green mussel (perna viridis) |
| title_full_unstemmed | Isolation and characterization of adhesive protein from green mussel (perna viridis) |
| title_short | Isolation and characterization of adhesive protein from green mussel (perna viridis) |
| title_sort | isolation and characterization of adhesive protein from green mussel (perna viridis) |
| topic | TP Chemical technology |
| url | http://eprints.utm.my/2663/1/71451.pdf http://eprints.utm.my/2663/ |
| url_provider | http://eprints.utm.my/ |