Application of sol gel technique for glucose oxidase immobilization in biosensor application
A sol gel based biosensor using 3-glycidoxypropyl dimethylethoxysilane, as a silane agent was developed as a novel method for biosensor enzyme immobilization. The key materials applied were tetra methyl orthosilicate (TMOS), and the cross link agent, 3-Glycidoxypropyl dimethylethoxysilane, (GDP). Th...
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| Format: | Conference or Workshop Item |
| Language: | en |
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2006
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| Online Access: | http://eprints.utm.my/209/1/NashrulFazliMohdNasir2006_Applicationofsolgeltechnique.pdf http://eprints.utm.my/209/ |
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| author | Mohd. Nasir, Nashrul Fazli Ying, Yang Che Ahmad, Azlan Kadri, Nahrizul Adib |
| author_facet | Mohd. Nasir, Nashrul Fazli Ying, Yang Che Ahmad, Azlan Kadri, Nahrizul Adib |
| author_sort | Mohd. Nasir, Nashrul Fazli |
| building | UTM Library |
| collection | Institutional Repository |
| content_provider | Universiti Teknologi Malaysia |
| content_source | UTM Institutional Repository |
| continent | Asia |
| country | Malaysia |
| description | A sol gel based biosensor using 3-glycidoxypropyl dimethylethoxysilane, as a silane agent was developed as a novel method for biosensor enzyme immobilization. The key materials applied were tetra methyl orthosilicate (TMOS), and the cross link agent, 3-Glycidoxypropyl dimethylethoxysilane, (GDP). Three compositions with different amount of GDP and glucose oxidase were experimented with the amount of TMOS was kept constant. The first coating consisted of 2mg of glucose oxidase and 20µl of GDP. The second trial involved higher GDP composition which is 100µl. The final trial applied higher content of glucose oxidase which is 12 mg with 20µl of GDP. The result showed that sensors with high composition of crosslink agent coating was capable to exhibit reliable glucose detection. The crosslink agent insufficiency in the first composition failed to provide good attachment for the enzyme on the electrode. Thus, the test was halted after few readings due to the inability of the sensor to detect glucose increment. For the high amount glucose oxidase composition, failure happened due to the deficiency of GDP to retain the enzyme thus contributed to the glucose oxidase leaching. We conclude that adequate amount of crosslink agent is vital for a sol gel based biosensor to function successfully. |
| format | Conference or Workshop Item |
| id | my.utm.eprints-209 |
| institution | Universiti Teknologi Malaysia |
| language | en |
| publishDate | 2006 |
| record_format | eprints |
| spelling | my.utm.eprints-2092017-08-24T00:20:10Z http://eprints.utm.my/209/ Application of sol gel technique for glucose oxidase immobilization in biosensor application Mohd. Nasir, Nashrul Fazli Ying, Yang Che Ahmad, Azlan Kadri, Nahrizul Adib TP Chemical technology A sol gel based biosensor using 3-glycidoxypropyl dimethylethoxysilane, as a silane agent was developed as a novel method for biosensor enzyme immobilization. The key materials applied were tetra methyl orthosilicate (TMOS), and the cross link agent, 3-Glycidoxypropyl dimethylethoxysilane, (GDP). Three compositions with different amount of GDP and glucose oxidase were experimented with the amount of TMOS was kept constant. The first coating consisted of 2mg of glucose oxidase and 20µl of GDP. The second trial involved higher GDP composition which is 100µl. The final trial applied higher content of glucose oxidase which is 12 mg with 20µl of GDP. The result showed that sensors with high composition of crosslink agent coating was capable to exhibit reliable glucose detection. The crosslink agent insufficiency in the first composition failed to provide good attachment for the enzyme on the electrode. Thus, the test was halted after few readings due to the inability of the sensor to detect glucose increment. For the high amount glucose oxidase composition, failure happened due to the deficiency of GDP to retain the enzyme thus contributed to the glucose oxidase leaching. We conclude that adequate amount of crosslink agent is vital for a sol gel based biosensor to function successfully. 2006-07 Conference or Workshop Item PeerReviewed application/pdf en http://eprints.utm.my/209/1/NashrulFazliMohdNasir2006_Applicationofsolgeltechnique.pdf Mohd. Nasir, Nashrul Fazli and Ying, Yang and Che Ahmad, Azlan and Kadri, Nahrizul Adib (2006) Application of sol gel technique for glucose oxidase immobilization in biosensor application. In: 1st International Conference on Natural Resources Engineering & Technology 2006, 24-25th July 2006, Putrajaya, Malaysia. |
| spellingShingle | TP Chemical technology Mohd. Nasir, Nashrul Fazli Ying, Yang Che Ahmad, Azlan Kadri, Nahrizul Adib Application of sol gel technique for glucose oxidase immobilization in biosensor application |
| title | Application of sol gel technique for glucose oxidase immobilization in biosensor application |
| title_full | Application of sol gel technique for glucose oxidase immobilization in biosensor application |
| title_fullStr | Application of sol gel technique for glucose oxidase immobilization in biosensor application |
| title_full_unstemmed | Application of sol gel technique for glucose oxidase immobilization in biosensor application |
| title_short | Application of sol gel technique for glucose oxidase immobilization in biosensor application |
| title_sort | application of sol gel technique for glucose oxidase immobilization in biosensor application |
| topic | TP Chemical technology |
| url | http://eprints.utm.my/209/1/NashrulFazliMohdNasir2006_Applicationofsolgeltechnique.pdf http://eprints.utm.my/209/ |
| url_provider | http://eprints.utm.my/ |