Separation Of Phenylarsonic Compounds By Ion Pairing-Reversed Phase-High Performance Liquid Chromatography
Phenylarsonic compounds are extensively used as feed additives to promote growth and to control disease in the poultry industry. A method was conducted that allowed the analysis of arsenic animal feed additives by ion pairing-reversed phase HPLC (IP-RP-HPLC) with a UV detection using an octadecylsil...
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| Language: | en |
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Penerbit UTM Press
2001
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| Online Access: | http://eprints.utm.my/1289/1/JT35C6.pdf http://eprints.utm.my/1289/ http://www.penerbit.utm.my/onlinejournal/35/C/JT35C6.pdf |
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| _version_ | 1845470437583093760 |
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| author | Jaafar, Jafariah |
| author_facet | Jaafar, Jafariah |
| author_sort | Jaafar, Jafariah |
| building | UTM Library |
| collection | Institutional Repository |
| content_provider | Universiti Teknologi Malaysia |
| content_source | UTM Institutional Repository |
| continent | Asia |
| country | Malaysia |
| description | Phenylarsonic compounds are extensively used as feed additives to promote growth and to control disease in the poultry industry. A method was conducted that allowed the analysis of arsenic animal feed additives by ion pairing-reversed phase HPLC (IP-RP-HPLC) with a UV detection using an octadecylsilylsilica column. The separation of anionic arsenic compounds [3-nitro-4-hydroxyphenylarsonic acid (3-NHPAA), p-arsanilic acid (p-ASA) and phenylarsonic acid (PA)] was accomplished using 5 mM tetrabutylammonium chloride as the ion pairing reagent in
aqueous mobile phase containing 3 mM malonic acid and 5% methanol at a flow rate of 1.4 mL min–1. The influence of mobile phase pH on the separation was studied; the optimum pH found was 5.85. The detection limit obtained for 3-NHPAA was 0.43 ng As.
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| format | Article |
| id | my.utm.eprints-1289 |
| institution | Universiti Teknologi Malaysia |
| language | en |
| publishDate | 2001 |
| publisher | Penerbit UTM Press |
| record_format | eprints |
| spelling | my.utm.eprints-12892017-11-01T04:17:46Z http://eprints.utm.my/1289/ Separation Of Phenylarsonic Compounds By Ion Pairing-Reversed Phase-High Performance Liquid Chromatography Jaafar, Jafariah QD Chemistry Phenylarsonic compounds are extensively used as feed additives to promote growth and to control disease in the poultry industry. A method was conducted that allowed the analysis of arsenic animal feed additives by ion pairing-reversed phase HPLC (IP-RP-HPLC) with a UV detection using an octadecylsilylsilica column. The separation of anionic arsenic compounds [3-nitro-4-hydroxyphenylarsonic acid (3-NHPAA), p-arsanilic acid (p-ASA) and phenylarsonic acid (PA)] was accomplished using 5 mM tetrabutylammonium chloride as the ion pairing reagent in aqueous mobile phase containing 3 mM malonic acid and 5% methanol at a flow rate of 1.4 mL min–1. The influence of mobile phase pH on the separation was studied; the optimum pH found was 5.85. The detection limit obtained for 3-NHPAA was 0.43 ng As. Penerbit UTM Press 2001-12 Article PeerReviewed application/pdf en http://eprints.utm.my/1289/1/JT35C6.pdf Jaafar, Jafariah (2001) Separation Of Phenylarsonic Compounds By Ion Pairing-Reversed Phase-High Performance Liquid Chromatography. Jurnal Teknologi C (35C). pp. 71-80. ISSN 0127-9696 http://www.penerbit.utm.my/onlinejournal/35/C/JT35C6.pdf |
| spellingShingle | QD Chemistry Jaafar, Jafariah Separation Of Phenylarsonic Compounds By Ion Pairing-Reversed Phase-High Performance Liquid Chromatography |
| title | Separation Of Phenylarsonic Compounds By Ion Pairing-Reversed Phase-High Performance Liquid Chromatography |
| title_full | Separation Of Phenylarsonic Compounds By Ion Pairing-Reversed Phase-High Performance Liquid Chromatography |
| title_fullStr | Separation Of Phenylarsonic Compounds By Ion Pairing-Reversed Phase-High Performance Liquid Chromatography |
| title_full_unstemmed | Separation Of Phenylarsonic Compounds By Ion Pairing-Reversed Phase-High Performance Liquid Chromatography |
| title_short | Separation Of Phenylarsonic Compounds By Ion Pairing-Reversed Phase-High Performance Liquid Chromatography |
| title_sort | separation of phenylarsonic compounds by ion pairing-reversed phase-high performance liquid chromatography |
| topic | QD Chemistry |
| url | http://eprints.utm.my/1289/1/JT35C6.pdf http://eprints.utm.my/1289/ http://www.penerbit.utm.my/onlinejournal/35/C/JT35C6.pdf |
| url_provider | http://eprints.utm.my/ |