Investigation on hela cells behaviour induced with pulse electric field for wound healing application
This study focuses on the investigation of pulsed electric field (PEF) exposure effect on HeLa cells (cervical cancer cells) for in-vitro experiments. The study focused mainly on real time experimental setup for cell morphological properties imaging. In the experimental setup, a modified EC magne...
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| Language: | en en en |
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2017
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| Online Access: | http://eprints.uthm.edu.my/8504/2/24p%20MOHAMED%20AHMED%20MILDAD%20ZALTUM.pdf http://eprints.uthm.edu.my/8504/1/MOHAMED%20AHMED%20MILDAD%20ZALTUM%20COPYRIGHT%20DECLARATION.pdf http://eprints.uthm.edu.my/8504/3/MOHAMED%20AHMED%20MILDAD%20ZALTUM%20WATERMARK.pdf http://eprints.uthm.edu.my/8504/ |
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| _version_ | 1833418685853728768 |
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| author | Milad Zaltum, Mohamed Ahmed |
| author_facet | Milad Zaltum, Mohamed Ahmed |
| author_sort | Milad Zaltum, Mohamed Ahmed |
| building | UTHM Library |
| collection | Institutional Repository |
| content_provider | Universiti Tun Hussein Onn Malaysia |
| content_source | UTHM Institutional Repository |
| continent | Asia |
| country | Malaysia |
| description | This study focuses on the investigation of pulsed electric field (PEF) exposure effect on
HeLa cells (cervical cancer cells) for in-vitro experiments. The study focused mainly on
real time experimental setup for cell morphological properties imaging. In the
experimental setup, a modified EC magnetic chamber with incubator system is used to
maintain the real time in-vitro environment for exposing the HeLa cells to high electric
field. A Nikon inverted microscope (Ti-series) with Metamorph® time lapse application
is utlized for image capturing and video recording. The first investigation is to look at the
proliferation rate of HeLa cells within 72 hours inside the modified chamber. This first
investigation is utmost important to see if there is any effect from the PEF exposure. From
this, it was found that the HeLa cell growth rate increased up to 50% faster when applied
Electroporation (EP) in comparison to the cell without EP treatment. The investigation
was continued to look at the best PEF parameter that assisted in the growth rate of HeLa
cells. These investegations motivates the need of finding the best parameters for PEF
exposure that are suitable for HeLa cell reversible condition. This study continues to look
at the relation between amplitude and duration of PEF effect on the HeLa cell growth rate.
The cells were subjected to single pulse, constant field strength of l kV/cm and pulse
durations ranging from 30μs to 600μs. It was found that at l00μs pulse duration the HeLa
cell growth rate increased dramatically and achieving confluency faster in comparison to
the cells exposed with other pulse durations. After obtaining the best parameter for HeLa
cell ( l kV/cm, l00μs, & single pulse) the potential application of the EP technique for
wound healing was explored. The result of the exposed cells to PEF revealed a five times
faster healing rate than control group. Additionally, we have used Microcontact printing
(MCP) technique for cell guidance and assembling. The results indicates that the cells
aligned and elongated more on fibronectin pattern substrate under PEF than without PEF.
Thus, PEF usage on biological cells would enable a novel method for assisting drug free
wound repair systems and many other potential biomedical engineering applications. |
| format | Thesis |
| id | my.uthm.eprints-8504 |
| institution | Universiti Tun Hussein Onn Malaysia |
| language | en en en |
| publishDate | 2017 |
| record_format | eprints |
| spelling | my.uthm.eprints-85042023-04-02T01:23:54Z http://eprints.uthm.edu.my/8504/ Investigation on hela cells behaviour induced with pulse electric field for wound healing application Milad Zaltum, Mohamed Ahmed R Medicine (General) R131-687 History of medicine. Medical expeditions This study focuses on the investigation of pulsed electric field (PEF) exposure effect on HeLa cells (cervical cancer cells) for in-vitro experiments. The study focused mainly on real time experimental setup for cell morphological properties imaging. In the experimental setup, a modified EC magnetic chamber with incubator system is used to maintain the real time in-vitro environment for exposing the HeLa cells to high electric field. A Nikon inverted microscope (Ti-series) with Metamorph® time lapse application is utlized for image capturing and video recording. The first investigation is to look at the proliferation rate of HeLa cells within 72 hours inside the modified chamber. This first investigation is utmost important to see if there is any effect from the PEF exposure. From this, it was found that the HeLa cell growth rate increased up to 50% faster when applied Electroporation (EP) in comparison to the cell without EP treatment. The investigation was continued to look at the best PEF parameter that assisted in the growth rate of HeLa cells. These investegations motivates the need of finding the best parameters for PEF exposure that are suitable for HeLa cell reversible condition. This study continues to look at the relation between amplitude and duration of PEF effect on the HeLa cell growth rate. The cells were subjected to single pulse, constant field strength of l kV/cm and pulse durations ranging from 30μs to 600μs. It was found that at l00μs pulse duration the HeLa cell growth rate increased dramatically and achieving confluency faster in comparison to the cells exposed with other pulse durations. After obtaining the best parameter for HeLa cell ( l kV/cm, l00μs, & single pulse) the potential application of the EP technique for wound healing was explored. The result of the exposed cells to PEF revealed a five times faster healing rate than control group. Additionally, we have used Microcontact printing (MCP) technique for cell guidance and assembling. The results indicates that the cells aligned and elongated more on fibronectin pattern substrate under PEF than without PEF. Thus, PEF usage on biological cells would enable a novel method for assisting drug free wound repair systems and many other potential biomedical engineering applications. 2017-04 Thesis NonPeerReviewed text en http://eprints.uthm.edu.my/8504/2/24p%20MOHAMED%20AHMED%20MILDAD%20ZALTUM.pdf text en http://eprints.uthm.edu.my/8504/1/MOHAMED%20AHMED%20MILDAD%20ZALTUM%20COPYRIGHT%20DECLARATION.pdf text en http://eprints.uthm.edu.my/8504/3/MOHAMED%20AHMED%20MILDAD%20ZALTUM%20WATERMARK.pdf Milad Zaltum, Mohamed Ahmed (2017) Investigation on hela cells behaviour induced with pulse electric field for wound healing application. Doctoral thesis, Universiti Tun Hussein Onn Malaysia. |
| spellingShingle | R Medicine (General) R131-687 History of medicine. Medical expeditions Milad Zaltum, Mohamed Ahmed Investigation on hela cells behaviour induced with pulse electric field for wound healing application |
| title | Investigation on hela cells behaviour induced with pulse electric field for wound healing application |
| title_full | Investigation on hela cells behaviour induced with pulse electric field for wound healing application |
| title_fullStr | Investigation on hela cells behaviour induced with pulse electric field for wound healing application |
| title_full_unstemmed | Investigation on hela cells behaviour induced with pulse electric field for wound healing application |
| title_short | Investigation on hela cells behaviour induced with pulse electric field for wound healing application |
| title_sort | investigation on hela cells behaviour induced with pulse electric field for wound healing application |
| topic | R Medicine (General) R131-687 History of medicine. Medical expeditions |
| url | http://eprints.uthm.edu.my/8504/2/24p%20MOHAMED%20AHMED%20MILDAD%20ZALTUM.pdf http://eprints.uthm.edu.my/8504/1/MOHAMED%20AHMED%20MILDAD%20ZALTUM%20COPYRIGHT%20DECLARATION.pdf http://eprints.uthm.edu.my/8504/3/MOHAMED%20AHMED%20MILDAD%20ZALTUM%20WATERMARK.pdf http://eprints.uthm.edu.my/8504/ |
| url_provider | http://eprints.uthm.edu.my/ |