Study of effect of microsecond pulsed electric fields on threshold area of HeLa Cells
Microsecond pulse electric field (IlSPEF) application development substantially affected the development of research process including controlling cell functions by using pulses of electrical fields to create pores through a cellular membrane causes cell lysis and apoptosis commonly known as...
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| Language: | en |
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2012
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| Online Access: | http://eprints.uthm.edu.my/8038/1/P8719_9b82f6023c3876b190d1c21aacd6601f.pdf http://eprints.uthm.edu.my/8038/ |
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| author | Adon, Mohamad Nazib Dalimin, M. Noh Abdul Jamill, Muhammad Mahadi Mohd Kassim, Norazan Hamdan, Sallehhuddin |
| author_facet | Adon, Mohamad Nazib Dalimin, M. Noh Abdul Jamill, Muhammad Mahadi Mohd Kassim, Norazan Hamdan, Sallehhuddin |
| author_sort | Adon, Mohamad Nazib |
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| collection | Institutional Repository |
| content_provider | Universiti Tun Hussein Onn Malaysia |
| content_source | UTHM Institutional Repository |
| continent | Asia |
| country | Malaysia |
| description | Microsecond pulse electric field (IlSPEF) application
development substantially affected the development of research
process including controlling cell functions by using pulses of
electrical fields to create pores through a cellular membrane
causes cell lysis and apoptosis commonly known as
electroporation. Here we demonstrate the influence of the IlSPEF
on the threshold area (TA) of human cervical cancer cells (HeLa)
membrane. The electric field for IlSPEF is 3kV/cm while the pulse
interval is lOOms. The pulse length and the number of pulses were
fixed at lOllS and 5, respectively. While the cultured skin cells are
placed in 9 mm-gap EP electrode chamber for allowing real time
observation of membrane permeability changes and cellular
physiology. In order to initiate higher cell viability rate, high
transfection efficiency, lower sample contamination and smaller
Joule heating effect the modification of EP chamber need to be
done which can be controlled by pH scale, temperature and
humidity. The experiment using high pulse electrical field with
simply repetitive pulses shows the threshold area of cell
membrane was decreasing gradually to 44.59Ilm, and is settled
within hundreds of second. We found that the threshold area of
cells membrane was affected when exposed to high voltage pulse
electric field. The dependence of the threshold area on the HeLa
cell membrane might be associated with the electrical impedance
of the plasma membrane that begins to fluctuate after the
application of a certain level of IlSPEF. |
| format | Conference or Workshop Item |
| id | my.uthm.eprints-8038 |
| institution | Universiti Tun Hussein Onn Malaysia |
| language | en |
| publishDate | 2012 |
| record_format | eprints |
| spelling | my.uthm.eprints-80382022-12-06T02:51:20Z http://eprints.uthm.edu.my/8038/ Study of effect of microsecond pulsed electric fields on threshold area of HeLa Cells Adon, Mohamad Nazib Dalimin, M. Noh Abdul Jamill, Muhammad Mahadi Mohd Kassim, Norazan Hamdan, Sallehhuddin T Technology (General) Microsecond pulse electric field (IlSPEF) application development substantially affected the development of research process including controlling cell functions by using pulses of electrical fields to create pores through a cellular membrane causes cell lysis and apoptosis commonly known as electroporation. Here we demonstrate the influence of the IlSPEF on the threshold area (TA) of human cervical cancer cells (HeLa) membrane. The electric field for IlSPEF is 3kV/cm while the pulse interval is lOOms. The pulse length and the number of pulses were fixed at lOllS and 5, respectively. While the cultured skin cells are placed in 9 mm-gap EP electrode chamber for allowing real time observation of membrane permeability changes and cellular physiology. In order to initiate higher cell viability rate, high transfection efficiency, lower sample contamination and smaller Joule heating effect the modification of EP chamber need to be done which can be controlled by pH scale, temperature and humidity. The experiment using high pulse electrical field with simply repetitive pulses shows the threshold area of cell membrane was decreasing gradually to 44.59Ilm, and is settled within hundreds of second. We found that the threshold area of cells membrane was affected when exposed to high voltage pulse electric field. The dependence of the threshold area on the HeLa cell membrane might be associated with the electrical impedance of the plasma membrane that begins to fluctuate after the application of a certain level of IlSPEF. 2012 Conference or Workshop Item PeerReviewed text en http://eprints.uthm.edu.my/8038/1/P8719_9b82f6023c3876b190d1c21aacd6601f.pdf Adon, Mohamad Nazib and Dalimin, M. Noh and Abdul Jamill, Muhammad Mahadi and Mohd Kassim, Norazan and Hamdan, Sallehhuddin (2012) Study of effect of microsecond pulsed electric fields on threshold area of HeLa Cells. In: 2012 IEEE EMBS International Conference on Biomedical Engineering and Sciences, 17th - 19th December 2012, Langkawi. |
| spellingShingle | T Technology (General) Adon, Mohamad Nazib Dalimin, M. Noh Abdul Jamill, Muhammad Mahadi Mohd Kassim, Norazan Hamdan, Sallehhuddin Study of effect of microsecond pulsed electric fields on threshold area of HeLa Cells |
| title | Study of effect of microsecond pulsed electric fields on threshold area of HeLa Cells |
| title_full | Study of effect of microsecond pulsed electric fields on threshold area of HeLa Cells |
| title_fullStr | Study of effect of microsecond pulsed electric fields on threshold area of HeLa Cells |
| title_full_unstemmed | Study of effect of microsecond pulsed electric fields on threshold area of HeLa Cells |
| title_short | Study of effect of microsecond pulsed electric fields on threshold area of HeLa Cells |
| title_sort | study of effect of microsecond pulsed electric fields on threshold area of hela cells |
| topic | T Technology (General) |
| url | http://eprints.uthm.edu.my/8038/1/P8719_9b82f6023c3876b190d1c21aacd6601f.pdf http://eprints.uthm.edu.my/8038/ |
| url_provider | http://eprints.uthm.edu.my/ |