Short Communication: Effectiveness of nuclear gene in species and subspecies determination of captive orangutans
Abdul-Manan MN, Mohd-Ridwan AR, Aifat NR, Osman NA, Abdul-Latiff MA, Dharmalingam S, Md-Zain BM. 2020. Short Communication: Effectiveness of nuclear gene in species and subspecies determination of captive orangutans. Biodiversitas 21: 3665-3669. Genetic identification of captive orangutans is of par...
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2020
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| Online Access: | http://eprints.uthm.edu.my/6859/1/AJ%202020%20%28450%29.pdf http://eprints.uthm.edu.my/6859/ https://doi.org/10.13057/biodiv/d210832 |
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| author | ABDUL-MANAN, MUHAMMAD NURISMADEE MOHD-RIDWAN, ABD RAHMAN AIFAT, NOR RAHMAN OSMAN, NUR AZIMAH Abdul-Latiff, Muhammad Abu Bakar DHARMALINGAM, SABAPATHY MD-ZAIN, BADRUL MUNIR |
| author_facet | ABDUL-MANAN, MUHAMMAD NURISMADEE MOHD-RIDWAN, ABD RAHMAN AIFAT, NOR RAHMAN OSMAN, NUR AZIMAH Abdul-Latiff, Muhammad Abu Bakar DHARMALINGAM, SABAPATHY MD-ZAIN, BADRUL MUNIR |
| author_sort | ABDUL-MANAN, MUHAMMAD NURISMADEE |
| building | UTHM Library |
| collection | Institutional Repository |
| content_provider | Universiti Tun Hussein Onn Malaysia |
| content_source | UTHM Institutional Repository |
| continent | Asia |
| country | Malaysia |
| description | Abdul-Manan MN, Mohd-Ridwan AR, Aifat NR, Osman NA, Abdul-Latiff MA, Dharmalingam S, Md-Zain BM. 2020. Short Communication: Effectiveness of nuclear gene in species and subspecies determination of captive orangutans. Biodiversitas 21: 3665-3669. Genetic identification of captive orangutans is of paramount importance in providing a correct identity that is essential for captive management. Thus, the utility of nuclear DNA sequences was tested in this study to identify the genetic identity of captive orangutans at Bukit Merah Orang Utan Island. Out of 24 DNA samples that were successfully extracted, only 10 orangutan samples were successfully sequenced for the von Willebrand factor (vWF) gene. From the results, this gene was able to separate the genus Pongo at the species level. Distance and character analyses indicated that a clear separation between P. pygmaeus and P. abelii at the species level. However, the degree of separation at species level was indicated in tree topology with moderate bootstrap values. At the subspecies level of P. pygmaeus, this gene was unable to show a clear separation between three Bornean subspecies. All the subspecies were formed clade together with each other. The vMF gene is a good nuclear gene for the study of phylogenetic relationships of orangutans in captivity at the species level, but the genetic identification at subspecies level in the genus level remains unclear. We suggest that future studies should involve multiple independent nuclear markers to increase the probability of getting reliable results. |
| format | Article |
| id | my.uthm.eprints-6859 |
| institution | Universiti Tun Hussein Onn Malaysia |
| language | en |
| publishDate | 2020 |
| record_format | eprints |
| spelling | my.uthm.eprints-68592022-04-05T06:23:45Z http://eprints.uthm.edu.my/6859/ Short Communication: Effectiveness of nuclear gene in species and subspecies determination of captive orangutans ABDUL-MANAN, MUHAMMAD NURISMADEE MOHD-RIDWAN, ABD RAHMAN AIFAT, NOR RAHMAN OSMAN, NUR AZIMAH Abdul-Latiff, Muhammad Abu Bakar DHARMALINGAM, SABAPATHY MD-ZAIN, BADRUL MUNIR TP155-156 Chemical engineering Abdul-Manan MN, Mohd-Ridwan AR, Aifat NR, Osman NA, Abdul-Latiff MA, Dharmalingam S, Md-Zain BM. 2020. Short Communication: Effectiveness of nuclear gene in species and subspecies determination of captive orangutans. Biodiversitas 21: 3665-3669. Genetic identification of captive orangutans is of paramount importance in providing a correct identity that is essential for captive management. Thus, the utility of nuclear DNA sequences was tested in this study to identify the genetic identity of captive orangutans at Bukit Merah Orang Utan Island. Out of 24 DNA samples that were successfully extracted, only 10 orangutan samples were successfully sequenced for the von Willebrand factor (vWF) gene. From the results, this gene was able to separate the genus Pongo at the species level. Distance and character analyses indicated that a clear separation between P. pygmaeus and P. abelii at the species level. However, the degree of separation at species level was indicated in tree topology with moderate bootstrap values. At the subspecies level of P. pygmaeus, this gene was unable to show a clear separation between three Bornean subspecies. All the subspecies were formed clade together with each other. The vMF gene is a good nuclear gene for the study of phylogenetic relationships of orangutans in captivity at the species level, but the genetic identification at subspecies level in the genus level remains unclear. We suggest that future studies should involve multiple independent nuclear markers to increase the probability of getting reliable results. 2020 Article PeerReviewed text en http://eprints.uthm.edu.my/6859/1/AJ%202020%20%28450%29.pdf ABDUL-MANAN, MUHAMMAD NURISMADEE and MOHD-RIDWAN, ABD RAHMAN and AIFAT, NOR RAHMAN and OSMAN, NUR AZIMAH and Abdul-Latiff, Muhammad Abu Bakar and DHARMALINGAM, SABAPATHY and MD-ZAIN, BADRUL MUNIR (2020) Short Communication: Effectiveness of nuclear gene in species and subspecies determination of captive orangutans. BIODIVERSITAS, 21 (8). pp. 3665-3669. ISSN 1412-033X https://doi.org/10.13057/biodiv/d210832 |
| spellingShingle | TP155-156 Chemical engineering ABDUL-MANAN, MUHAMMAD NURISMADEE MOHD-RIDWAN, ABD RAHMAN AIFAT, NOR RAHMAN OSMAN, NUR AZIMAH Abdul-Latiff, Muhammad Abu Bakar DHARMALINGAM, SABAPATHY MD-ZAIN, BADRUL MUNIR Short Communication: Effectiveness of nuclear gene in species and subspecies determination of captive orangutans |
| title | Short Communication: Effectiveness of nuclear gene in species and subspecies determination of captive orangutans |
| title_full | Short Communication: Effectiveness of nuclear gene in species and subspecies determination of captive orangutans |
| title_fullStr | Short Communication: Effectiveness of nuclear gene in species and subspecies determination of captive orangutans |
| title_full_unstemmed | Short Communication: Effectiveness of nuclear gene in species and subspecies determination of captive orangutans |
| title_short | Short Communication: Effectiveness of nuclear gene in species and subspecies determination of captive orangutans |
| title_sort | short communication: effectiveness of nuclear gene in species and subspecies determination of captive orangutans |
| topic | TP155-156 Chemical engineering |
| url | http://eprints.uthm.edu.my/6859/1/AJ%202020%20%28450%29.pdf http://eprints.uthm.edu.my/6859/ https://doi.org/10.13057/biodiv/d210832 |
| url_provider | http://eprints.uthm.edu.my/ |