Enzymatic hydrolysis of thermally pre-treated chitin and antimicrobial activity of N,N′-diacetylchitobiose
Background: n,n′-diacetylchitobiose (glc n ac2) is known to be highly functional and offers a wide range of applications, especially as an antimicrobial agent. In this study, a thermal pre-treatment process using steam under pressure in an autoclave was employed to facilitate subsequent enzymatic hy...
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2019
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| Online Access: | http://eprints.uthm.edu.my/6136/1/AJ%202020%20%28207%29.pdf http://eprints.uthm.edu.my/6136/ https://dx.doi.org/10.1002/jctb.6043 |
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| author | Z Abidin, Munira Kourmentza, Constantina K Karatzas, Andreas Niranjan, Keshavan |
| author_facet | Z Abidin, Munira Kourmentza, Constantina K Karatzas, Andreas Niranjan, Keshavan |
| author_sort | Z Abidin, Munira |
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| content_provider | Universiti Tun Hussein Onn Malaysia |
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| continent | Asia |
| country | Malaysia |
| description | Background: n,n′-diacetylchitobiose (glc n ac2) is known to be highly functional and offers a wide range of applications, especially as an antimicrobial agent. In this study, a thermal pre-treatment process using steam under pressure in an autoclave was employed to facilitate subsequent enzymatic hydrolysis of chitin with chitinase from streptomyces griseus. Results: pre-treatment of chitin with 0.05mol l−1 sodium acetate buffer (p h= 6.0) at 121 ∘c for 60 min, followed by enzymatic hydrolysis involving24 hincubation,were foundtobethebest conditions for producing glc n ac2. the glc n ac2 thus obtainedwas tested regarding its antimicrobial activity against gram-negative and gram-positive strains and showed minimum inhibitory concentrations at 5 and 10% (w/v) against escherichia coli k-12 and listeria monocytogenes 10403 s, respectively. Conclusions: the extent of swelling and crystallite size of chitin increased with pre-treatment residence time, and enhanced the rate of subsequent hydrolysis using chitinase. © 2019 society of chemical industry |
| format | Article |
| id | my.uthm.eprints-6136 |
| institution | Universiti Tun Hussein Onn Malaysia |
| language | en |
| publishDate | 2019 |
| publisher | Wiley |
| record_format | eprints |
| spelling | my.uthm.eprints-61362022-01-26T08:53:02Z http://eprints.uthm.edu.my/6136/ Enzymatic hydrolysis of thermally pre-treated chitin and antimicrobial activity of N,N′-diacetylchitobiose Z Abidin, Munira Kourmentza, Constantina K Karatzas, Andreas Niranjan, Keshavan TP Chemical technology Background: n,n′-diacetylchitobiose (glc n ac2) is known to be highly functional and offers a wide range of applications, especially as an antimicrobial agent. In this study, a thermal pre-treatment process using steam under pressure in an autoclave was employed to facilitate subsequent enzymatic hydrolysis of chitin with chitinase from streptomyces griseus. Results: pre-treatment of chitin with 0.05mol l−1 sodium acetate buffer (p h= 6.0) at 121 ∘c for 60 min, followed by enzymatic hydrolysis involving24 hincubation,were foundtobethebest conditions for producing glc n ac2. the glc n ac2 thus obtainedwas tested regarding its antimicrobial activity against gram-negative and gram-positive strains and showed minimum inhibitory concentrations at 5 and 10% (w/v) against escherichia coli k-12 and listeria monocytogenes 10403 s, respectively. Conclusions: the extent of swelling and crystallite size of chitin increased with pre-treatment residence time, and enhanced the rate of subsequent hydrolysis using chitinase. © 2019 society of chemical industry Wiley 2019 Article PeerReviewed text en http://eprints.uthm.edu.my/6136/1/AJ%202020%20%28207%29.pdf Z Abidin, Munira and Kourmentza, Constantina and K Karatzas, Andreas and Niranjan, Keshavan (2019) Enzymatic hydrolysis of thermally pre-treated chitin and antimicrobial activity of N,N′-diacetylchitobiose. Journal of Chemical Technology and Biotechnology, 94 (8). pp. 2529-2536. ISSN 0268-2575 https://dx.doi.org/10.1002/jctb.6043 |
| spellingShingle | TP Chemical technology Z Abidin, Munira Kourmentza, Constantina K Karatzas, Andreas Niranjan, Keshavan Enzymatic hydrolysis of thermally pre-treated chitin and antimicrobial activity of N,N′-diacetylchitobiose |
| title | Enzymatic hydrolysis of thermally pre-treated chitin and antimicrobial activity of N,N′-diacetylchitobiose |
| title_full | Enzymatic hydrolysis of thermally pre-treated chitin and antimicrobial activity of N,N′-diacetylchitobiose |
| title_fullStr | Enzymatic hydrolysis of thermally pre-treated chitin and antimicrobial activity of N,N′-diacetylchitobiose |
| title_full_unstemmed | Enzymatic hydrolysis of thermally pre-treated chitin and antimicrobial activity of N,N′-diacetylchitobiose |
| title_short | Enzymatic hydrolysis of thermally pre-treated chitin and antimicrobial activity of N,N′-diacetylchitobiose |
| title_sort | enzymatic hydrolysis of thermally pre-treated chitin and antimicrobial activity of n,n′-diacetylchitobiose |
| topic | TP Chemical technology |
| url | http://eprints.uthm.edu.my/6136/1/AJ%202020%20%28207%29.pdf http://eprints.uthm.edu.my/6136/ https://dx.doi.org/10.1002/jctb.6043 |
| url_provider | http://eprints.uthm.edu.my/ |