Isolation of a pigment-producing bacterium chryseobacterium sp. from the tentacle of holothuria (lessonothuria) pardalis (Selenka 1867) from Pulau Tinggi, Johor
Chryseobacterium sp, a bacteria producing pigment was isolated from the tentacles of Holothuria (Lessonothuria) pardalis (Selenka 1867) from Pulau Tinggi, Johor. This study aimed to observe and genetically identified the presence of bacteria focusing on pigmentproducing bacteria. Thus, the partial 1...
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| author | Siti Najihah Solehin, Siti Najihah Solehin Nor Shahida Ab Rahman, Nor Shahida Ab Rahman Muhammad Abdul Latiff Abu Bakar, Muhammad Abdul Latiff Abu Bakar Aisyah Mohamed Rehan, Aisyah Mohamed Rehan Kamarul Rahim Kamarudin, Kamarul Rahim Kamarudin |
| author_facet | Siti Najihah Solehin, Siti Najihah Solehin Nor Shahida Ab Rahman, Nor Shahida Ab Rahman Muhammad Abdul Latiff Abu Bakar, Muhammad Abdul Latiff Abu Bakar Aisyah Mohamed Rehan, Aisyah Mohamed Rehan Kamarul Rahim Kamarudin, Kamarul Rahim Kamarudin |
| author_sort | Siti Najihah Solehin, Siti Najihah Solehin |
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| collection | Institutional Repository |
| content_provider | Universiti Tun Hussein Onn Malaysia |
| content_source | UTHM Institutional Repository |
| continent | Asia |
| country | Malaysia |
| description | Chryseobacterium sp, a bacteria producing pigment was isolated from the tentacles of Holothuria (Lessonothuria) pardalis (Selenka 1867) from Pulau Tinggi, Johor. This study aimed to observe and genetically identified the presence of bacteria focusing on pigmentproducing bacteria. Thus, the partial 16S ribosomal RNA gene sequence of bacteria obtained and the shape, number of bacteria and gram staining observed using a digital compound microscope. Six gene sequence successfully obtained and the Basic Local Alignment Search Tool (BLAST) analysis suggested three species of bacteria identified from genus Pseudomonas, Chryseobacterium and Klebsiella. Besides, the neighbourhood-joining and Maximum Likelihood method had been used in the phylogenetic analysis to support the identification of bacteria species. The visible spectra of Chryseobacterium sp. were recorded between 400nm to 600nm which is a visible spectrum for carotenoid; orange pigment. However, further analysis is needed to confirm the characterisation and the potential of this pigment in industrial |
| format | Conference or Workshop Item |
| id | my.uthm.eprints-6086 |
| institution | Universiti Tun Hussein Onn Malaysia |
| language | en |
| record_format | eprints |
| spelling | my.uthm.eprints-60862022-01-31T04:25:33Z http://eprints.uthm.edu.my/6086/ Isolation of a pigment-producing bacterium chryseobacterium sp. from the tentacle of holothuria (lessonothuria) pardalis (Selenka 1867) from Pulau Tinggi, Johor Siti Najihah Solehin, Siti Najihah Solehin Nor Shahida Ab Rahman, Nor Shahida Ab Rahman Muhammad Abdul Latiff Abu Bakar, Muhammad Abdul Latiff Abu Bakar Aisyah Mohamed Rehan, Aisyah Mohamed Rehan Kamarul Rahim Kamarudin, Kamarul Rahim Kamarudin TP248.13-248.65 Biotechnology Chryseobacterium sp, a bacteria producing pigment was isolated from the tentacles of Holothuria (Lessonothuria) pardalis (Selenka 1867) from Pulau Tinggi, Johor. This study aimed to observe and genetically identified the presence of bacteria focusing on pigmentproducing bacteria. Thus, the partial 16S ribosomal RNA gene sequence of bacteria obtained and the shape, number of bacteria and gram staining observed using a digital compound microscope. Six gene sequence successfully obtained and the Basic Local Alignment Search Tool (BLAST) analysis suggested three species of bacteria identified from genus Pseudomonas, Chryseobacterium and Klebsiella. Besides, the neighbourhood-joining and Maximum Likelihood method had been used in the phylogenetic analysis to support the identification of bacteria species. The visible spectra of Chryseobacterium sp. were recorded between 400nm to 600nm which is a visible spectrum for carotenoid; orange pigment. However, further analysis is needed to confirm the characterisation and the potential of this pigment in industrial Conference or Workshop Item PeerReviewed text en http://eprints.uthm.edu.my/6086/1/KP%202020%20%28110%29.pdf Siti Najihah Solehin, Siti Najihah Solehin and Nor Shahida Ab Rahman, Nor Shahida Ab Rahman and Muhammad Abdul Latiff Abu Bakar, Muhammad Abdul Latiff Abu Bakar and Aisyah Mohamed Rehan, Aisyah Mohamed Rehan and Kamarul Rahim Kamarudin, Kamarul Rahim Kamarudin Isolation of a pigment-producing bacterium chryseobacterium sp. from the tentacle of holothuria (lessonothuria) pardalis (Selenka 1867) from Pulau Tinggi, Johor. In: International Symposium of Straits of Melaka, 30 march 2020, Melaka, Malaysia. |
| spellingShingle | TP248.13-248.65 Biotechnology Siti Najihah Solehin, Siti Najihah Solehin Nor Shahida Ab Rahman, Nor Shahida Ab Rahman Muhammad Abdul Latiff Abu Bakar, Muhammad Abdul Latiff Abu Bakar Aisyah Mohamed Rehan, Aisyah Mohamed Rehan Kamarul Rahim Kamarudin, Kamarul Rahim Kamarudin Isolation of a pigment-producing bacterium chryseobacterium sp. from the tentacle of holothuria (lessonothuria) pardalis (Selenka 1867) from Pulau Tinggi, Johor |
| title | Isolation of a pigment-producing bacterium chryseobacterium sp. from the tentacle of holothuria (lessonothuria) pardalis (Selenka 1867) from Pulau Tinggi, Johor |
| title_full | Isolation of a pigment-producing bacterium chryseobacterium sp. from the tentacle of holothuria (lessonothuria) pardalis (Selenka 1867) from Pulau Tinggi, Johor |
| title_fullStr | Isolation of a pigment-producing bacterium chryseobacterium sp. from the tentacle of holothuria (lessonothuria) pardalis (Selenka 1867) from Pulau Tinggi, Johor |
| title_full_unstemmed | Isolation of a pigment-producing bacterium chryseobacterium sp. from the tentacle of holothuria (lessonothuria) pardalis (Selenka 1867) from Pulau Tinggi, Johor |
| title_short | Isolation of a pigment-producing bacterium chryseobacterium sp. from the tentacle of holothuria (lessonothuria) pardalis (Selenka 1867) from Pulau Tinggi, Johor |
| title_sort | isolation of a pigment-producing bacterium chryseobacterium sp. from the tentacle of holothuria (lessonothuria) pardalis (selenka 1867) from pulau tinggi, johor |
| topic | TP248.13-248.65 Biotechnology |
| url | http://eprints.uthm.edu.my/6086/1/KP%202020%20%28110%29.pdf http://eprints.uthm.edu.my/6086/ |
| url_provider | http://eprints.uthm.edu.my/ |