Optimising High-Pressure Processing (HPP) for Optimal Total Phenolic and Flavonoid Content in Kelulut (Stingless Bee) Honey
High-pressure processing (HPP) has the potential to enhance the total phenolic and flavonoid content (TPC and TFC) in Kelulut honey (KH). However, KH’s inherent variability possesses challenges in optimising these phytochemicals. This study hence explores the optimisation of KH through HPP, focusing...
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2024
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| Online Access: | http://eprints.uthm.edu.my/12510/1/J17995_403e4ac6662831e316f7a6a721970fd5.pdf http://eprints.uthm.edu.my/12510/ |
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| author | Razali, Muhammad Faiz A.Talip, Balkis Mohd Adzahan, Noranizan Sulaiman, Alifdalino Samiun, Azeman Mohd Fauzi, Noor Akhmazillah |
| author_facet | Razali, Muhammad Faiz A.Talip, Balkis Mohd Adzahan, Noranizan Sulaiman, Alifdalino Samiun, Azeman Mohd Fauzi, Noor Akhmazillah |
| author_sort | Razali, Muhammad Faiz |
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| content_provider | Universiti Tun Hussein Onn Malaysia |
| content_source | UTHM Institutional Repository |
| continent | Asia |
| country | Malaysia |
| description | High-pressure processing (HPP) has the potential to enhance the total phenolic and flavonoid content (TPC and TFC) in Kelulut honey (KH). However, KH’s inherent variability possesses challenges in optimising these phytochemicals. This study hence explores the optimisation of KH through HPP, focusing on TPC and TFC. The Facecentered Central Composite Design (FC-CCD), involving two factors with three levels each (pressure of 200, 400, and 600 MPa, and time
of 5, 10, and 15 min), was applied to investigate two response variables (total phenolic and flavonoid content). The optimisation identified the optimal parameters as 200 MPa for 15 min, with a desirability of 0.986, indicating precise modeling. Compared to other pressures (400 and 600 MPa) at common processing time (15 minutes), 200 MPa recorded higher TPC and TFC with differences of 25.71% to 30.92% and 13.78% to 14.19%, respectively. A verification step revealed that HPP-KH at 200 MPa for 15 minutes yielded a TPC of 15.278 ± 0.525 mg GAE/100 g and a TFC of 38.274 ± 1.980 mg RE/100 g, indicating the precise accuracy of the quadratic modeling. However, this discovery contradicts the consensus that pressures above 500 MPa increase TPC and TFC. Consequently, it underscores the need for tailored high-pressure strategies in KH processing, offering essential insights for industry applications and
further research endeavors. |
| format | Article |
| id | my.uthm.eprints-12510 |
| institution | Universiti Tun Hussein Onn Malaysia |
| language | en |
| publishDate | 2024 |
| record_format | eprints |
| spelling | my.uthm.eprints-125102025-05-05T06:33:31Z http://eprints.uthm.edu.my/12510/ Optimising High-Pressure Processing (HPP) for Optimal Total Phenolic and Flavonoid Content in Kelulut (Stingless Bee) Honey Razali, Muhammad Faiz A.Talip, Balkis Mohd Adzahan, Noranizan Sulaiman, Alifdalino Samiun, Azeman Mohd Fauzi, Noor Akhmazillah TP Chemical technology High-pressure processing (HPP) has the potential to enhance the total phenolic and flavonoid content (TPC and TFC) in Kelulut honey (KH). However, KH’s inherent variability possesses challenges in optimising these phytochemicals. This study hence explores the optimisation of KH through HPP, focusing on TPC and TFC. The Facecentered Central Composite Design (FC-CCD), involving two factors with three levels each (pressure of 200, 400, and 600 MPa, and time of 5, 10, and 15 min), was applied to investigate two response variables (total phenolic and flavonoid content). The optimisation identified the optimal parameters as 200 MPa for 15 min, with a desirability of 0.986, indicating precise modeling. Compared to other pressures (400 and 600 MPa) at common processing time (15 minutes), 200 MPa recorded higher TPC and TFC with differences of 25.71% to 30.92% and 13.78% to 14.19%, respectively. A verification step revealed that HPP-KH at 200 MPa for 15 minutes yielded a TPC of 15.278 ± 0.525 mg GAE/100 g and a TFC of 38.274 ± 1.980 mg RE/100 g, indicating the precise accuracy of the quadratic modeling. However, this discovery contradicts the consensus that pressures above 500 MPa increase TPC and TFC. Consequently, it underscores the need for tailored high-pressure strategies in KH processing, offering essential insights for industry applications and further research endeavors. 2024 Article PeerReviewed text en http://eprints.uthm.edu.my/12510/1/J17995_403e4ac6662831e316f7a6a721970fd5.pdf Razali, Muhammad Faiz and A.Talip, Balkis and Mohd Adzahan, Noranizan and Sulaiman, Alifdalino and Samiun, Azeman and Mohd Fauzi, Noor Akhmazillah (2024) Optimising High-Pressure Processing (HPP) for Optimal Total Phenolic and Flavonoid Content in Kelulut (Stingless Bee) Honey. Natural and Life Sciences Communications, 23 (3). pp. 1-14. |
| spellingShingle | TP Chemical technology Razali, Muhammad Faiz A.Talip, Balkis Mohd Adzahan, Noranizan Sulaiman, Alifdalino Samiun, Azeman Mohd Fauzi, Noor Akhmazillah Optimising High-Pressure Processing (HPP) for Optimal Total Phenolic and Flavonoid Content in Kelulut (Stingless Bee) Honey |
| title | Optimising High-Pressure Processing (HPP) for Optimal Total Phenolic and Flavonoid Content in Kelulut (Stingless Bee) Honey |
| title_full | Optimising High-Pressure Processing (HPP) for Optimal Total Phenolic and Flavonoid Content in Kelulut (Stingless Bee) Honey |
| title_fullStr | Optimising High-Pressure Processing (HPP) for Optimal Total Phenolic and Flavonoid Content in Kelulut (Stingless Bee) Honey |
| title_full_unstemmed | Optimising High-Pressure Processing (HPP) for Optimal Total Phenolic and Flavonoid Content in Kelulut (Stingless Bee) Honey |
| title_short | Optimising High-Pressure Processing (HPP) for Optimal Total Phenolic and Flavonoid Content in Kelulut (Stingless Bee) Honey |
| title_sort | optimising high-pressure processing (hpp) for optimal total phenolic and flavonoid content in kelulut (stingless bee) honey |
| topic | TP Chemical technology |
| url | http://eprints.uthm.edu.my/12510/1/J17995_403e4ac6662831e316f7a6a721970fd5.pdf http://eprints.uthm.edu.my/12510/ |
| url_provider | http://eprints.uthm.edu.my/ |