Molecular phylogeny confirms the subspecies delineation of the Malayan Siamang (Symphalangus syndactylus continentis) and the Sumatran Siamang (Symphalangus syndactylus syndactylus) based on the hypervariable region of mitochondrial DNA
Siamangs (Symphalangus syndactylus) are native to Peninsular Malaysia, Sumatra and southern Thailand and their taxonomical classification at subspecies level remains unclear. Morphologically, two subspecies were proposed as early as 1908 by Thomas namely Symphalangus s. syndactylus and Symphalangus...
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2024
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| Online Access: | http://eprints.uthm.edu.my/12132/1/J17691_537c0e807af054ba615621fb2add2a23.pdf http://eprints.uthm.edu.my/12132/ https://doi.org/10.3897/BDJ.12.e120314 |
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| author | Sariyati, Nur Hartini Abdul-Latiff, Muhammad Abu Bakar Aifat, Nor Rahman Mohd-Ridwan, Abd Rahman Osman, Nur Azimah Karuppannan, Kayal Vizi Chan, Eddie Md-Zain, Badrul Munir |
| author_facet | Sariyati, Nur Hartini Abdul-Latiff, Muhammad Abu Bakar Aifat, Nor Rahman Mohd-Ridwan, Abd Rahman Osman, Nur Azimah Karuppannan, Kayal Vizi Chan, Eddie Md-Zain, Badrul Munir |
| author_sort | Sariyati, Nur Hartini |
| building | UTHM Library |
| collection | Institutional Repository |
| content_provider | Universiti Tun Hussein Onn Malaysia |
| content_source | UTHM Institutional Repository |
| continent | Asia |
| country | Malaysia |
| description | Siamangs (Symphalangus syndactylus) are native to Peninsular Malaysia, Sumatra and southern Thailand and their taxonomical classification at subspecies level remains unclear. Morphologically, two subspecies were proposed as early as 1908 by Thomas namely Symphalangus s. syndactylus and Symphalangus s. continentis. Thus, this study aims to clarify the Siamang subspecies status, based on mtDNA D-loop sequences. Faecal samples were collected from wild Siamang populations at different localities in Peninsular Malaysia. A 600-bp sequence of the mitochondrial D-loop region was amplified from faecal DNA extracts and analysed along with GenBank sequences representing Symphalangus sp., Nomascus sp., Hylobates sp., Hoolock sp. and outgroups (Pongo pygmaeus, Macaca fascicularis and Papio papio). The molecular phylogenetic analysis in this study revealed two distinct clades formed by S. s. syndactylus and S. s. continentis which supports the previous morphological delineation of the existence of two subspecies. Biogeographical analysis indicated that the Sumatran population lineage was split from the Peninsular
Malaysian population lineage and a diversification occurrred in the Pliocene era (~ 3.12 MYA) through southward expansion. This postulation was supported by the molecular clock, which illustrated that the Peninsular Malaysian population (~ 1.92 MYA) diverged earlier than the Sumatran population (~ 1.85 MYA). This is the first study to use a molecular approach to validate the subspecies statuses of S. s. syndactylus and S. s.
continentis. This finding will be useful for conservation management, for example, during Siamang translocation and investigations into illegal pet trade and forensics involving
Malayan and Sumatran Siamangs. |
| format | Article |
| id | my.uthm.eprints-12132 |
| institution | Universiti Tun Hussein Onn Malaysia |
| language | en |
| publishDate | 2024 |
| record_format | eprints |
| spelling | my.uthm.eprints-121322025-04-24T03:10:30Z http://eprints.uthm.edu.my/12132/ Molecular phylogeny confirms the subspecies delineation of the Malayan Siamang (Symphalangus syndactylus continentis) and the Sumatran Siamang (Symphalangus syndactylus syndactylus) based on the hypervariable region of mitochondrial DNA Sariyati, Nur Hartini Abdul-Latiff, Muhammad Abu Bakar Aifat, Nor Rahman Mohd-Ridwan, Abd Rahman Osman, Nur Azimah Karuppannan, Kayal Vizi Chan, Eddie Md-Zain, Badrul Munir QL Zoology Siamangs (Symphalangus syndactylus) are native to Peninsular Malaysia, Sumatra and southern Thailand and their taxonomical classification at subspecies level remains unclear. Morphologically, two subspecies were proposed as early as 1908 by Thomas namely Symphalangus s. syndactylus and Symphalangus s. continentis. Thus, this study aims to clarify the Siamang subspecies status, based on mtDNA D-loop sequences. Faecal samples were collected from wild Siamang populations at different localities in Peninsular Malaysia. A 600-bp sequence of the mitochondrial D-loop region was amplified from faecal DNA extracts and analysed along with GenBank sequences representing Symphalangus sp., Nomascus sp., Hylobates sp., Hoolock sp. and outgroups (Pongo pygmaeus, Macaca fascicularis and Papio papio). The molecular phylogenetic analysis in this study revealed two distinct clades formed by S. s. syndactylus and S. s. continentis which supports the previous morphological delineation of the existence of two subspecies. Biogeographical analysis indicated that the Sumatran population lineage was split from the Peninsular Malaysian population lineage and a diversification occurrred in the Pliocene era (~ 3.12 MYA) through southward expansion. This postulation was supported by the molecular clock, which illustrated that the Peninsular Malaysian population (~ 1.92 MYA) diverged earlier than the Sumatran population (~ 1.85 MYA). This is the first study to use a molecular approach to validate the subspecies statuses of S. s. syndactylus and S. s. continentis. This finding will be useful for conservation management, for example, during Siamang translocation and investigations into illegal pet trade and forensics involving Malayan and Sumatran Siamangs. 2024 Article PeerReviewed text en http://eprints.uthm.edu.my/12132/1/J17691_537c0e807af054ba615621fb2add2a23.pdf Sariyati, Nur Hartini and Abdul-Latiff, Muhammad Abu Bakar and Aifat, Nor Rahman and Mohd-Ridwan, Abd Rahman and Osman, Nur Azimah and Karuppannan, Kayal Vizi and Chan, Eddie and Md-Zain, Badrul Munir (2024) Molecular phylogeny confirms the subspecies delineation of the Malayan Siamang (Symphalangus syndactylus continentis) and the Sumatran Siamang (Symphalangus syndactylus syndactylus) based on the hypervariable region of mitochondrial DNA. Biodiversity Data Journal, 12. pp. 1-24. https://doi.org/10.3897/BDJ.12.e120314 |
| spellingShingle | QL Zoology Sariyati, Nur Hartini Abdul-Latiff, Muhammad Abu Bakar Aifat, Nor Rahman Mohd-Ridwan, Abd Rahman Osman, Nur Azimah Karuppannan, Kayal Vizi Chan, Eddie Md-Zain, Badrul Munir Molecular phylogeny confirms the subspecies delineation of the Malayan Siamang (Symphalangus syndactylus continentis) and the Sumatran Siamang (Symphalangus syndactylus syndactylus) based on the hypervariable region of mitochondrial DNA |
| title | Molecular phylogeny confirms the subspecies delineation of the Malayan Siamang (Symphalangus syndactylus continentis) and the Sumatran Siamang (Symphalangus syndactylus syndactylus) based on the hypervariable region of mitochondrial DNA |
| title_full | Molecular phylogeny confirms the subspecies delineation of the Malayan Siamang (Symphalangus syndactylus continentis) and the Sumatran Siamang (Symphalangus syndactylus syndactylus) based on the hypervariable region of mitochondrial DNA |
| title_fullStr | Molecular phylogeny confirms the subspecies delineation of the Malayan Siamang (Symphalangus syndactylus continentis) and the Sumatran Siamang (Symphalangus syndactylus syndactylus) based on the hypervariable region of mitochondrial DNA |
| title_full_unstemmed | Molecular phylogeny confirms the subspecies delineation of the Malayan Siamang (Symphalangus syndactylus continentis) and the Sumatran Siamang (Symphalangus syndactylus syndactylus) based on the hypervariable region of mitochondrial DNA |
| title_short | Molecular phylogeny confirms the subspecies delineation of the Malayan Siamang (Symphalangus syndactylus continentis) and the Sumatran Siamang (Symphalangus syndactylus syndactylus) based on the hypervariable region of mitochondrial DNA |
| title_sort | molecular phylogeny confirms the subspecies delineation of the malayan siamang (symphalangus syndactylus continentis) and the sumatran siamang (symphalangus syndactylus syndactylus) based on the hypervariable region of mitochondrial dna |
| topic | QL Zoology |
| url | http://eprints.uthm.edu.my/12132/1/J17691_537c0e807af054ba615621fb2add2a23.pdf http://eprints.uthm.edu.my/12132/ https://doi.org/10.3897/BDJ.12.e120314 |
| url_provider | http://eprints.uthm.edu.my/ |