Comparison of metabarcoding techniques for dietary assessment in herbivores and omnivores
Dietary assessment plays a crucial role in comprehending the ecological dynamics and nutritional needs of herbivores and omnivores. The metabarcoding technique has emerged as a potent tool for exploring the dietary composition of these animals. However, various metabarcoding techniques have been dev...
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| Format: | Conference or Workshop Item |
| Language: | en |
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2023
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| Online Access: | http://eprints.uthm.edu.my/11232/1/P16740_7dd13dbc665a91a58657d0f3e2390e4b%204.pdf http://eprints.uthm.edu.my/11232/ https://doi.org/10.1051/bioconf/20249401002 |
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| author | Sariyati, Nur Hartini Haris, Hidayah Ramli, Farah Farhana Sufahani, Suliadi Firdaus Md Zain, Badrul Munir Yaakop, Salmah Mohd Ridwan, Abd Rahman Aifat, Nor Rahman Mohamed, Mohd Sanusi Maryanto, Ibnu Abdul Latiff, Muhammad Abu Bakar |
| author_facet | Sariyati, Nur Hartini Haris, Hidayah Ramli, Farah Farhana Sufahani, Suliadi Firdaus Md Zain, Badrul Munir Yaakop, Salmah Mohd Ridwan, Abd Rahman Aifat, Nor Rahman Mohamed, Mohd Sanusi Maryanto, Ibnu Abdul Latiff, Muhammad Abu Bakar |
| author_sort | Sariyati, Nur Hartini |
| building | UTHM Library |
| collection | Institutional Repository |
| content_provider | Universiti Tun Hussein Onn Malaysia |
| content_source | UTHM Institutional Repository |
| continent | Asia |
| country | Malaysia |
| description | Dietary assessment plays a crucial role in comprehending the ecological dynamics and nutritional needs of herbivores and omnivores. The metabarcoding technique has emerged as a potent tool for exploring the dietary composition of these animals. However, various metabarcoding techniques have been developed, each with its own advantages and limitations. This study aims to compare the performance of different metabarcoding techniques in herbivores and omnivores diet. We systematically reviewed 159 published manuscripts in Scopus and Google Scholar, and thematic analysis was conducted across several categories, including the marker, platform, and database utilized. Preliminary findings reveal significant variations among metabarcoding techniques across these two animal groups. The trnL gene exhibited higher taxonomic resolution for herbivorous species, whereas the combination of the 'trnL + 16s rRNA' gene exhibited superior performance for omnivorous species. The Illumina platform emerged as the most commonly used method for analyzing the diets of both herbivores and omnivores, with the primary reference database being the National Centre for Biotechnology Information (NCBI). This study offers valuable insights into the strengths and limitations of different metabarcoding techniques for dietary assessment in herbivores and omnivores and optimizing metabarcoding protocols, facilitating more precise and reliable diet analyses within these ecological groups |
| format | Conference or Workshop Item |
| id | my.uthm.eprints-11232 |
| institution | Universiti Tun Hussein Onn Malaysia |
| language | en |
| publishDate | 2023 |
| record_format | eprints |
| spelling | my.uthm.eprints-112322024-06-26T07:37:40Z http://eprints.uthm.edu.my/11232/ Comparison of metabarcoding techniques for dietary assessment in herbivores and omnivores Sariyati, Nur Hartini Haris, Hidayah Ramli, Farah Farhana Sufahani, Suliadi Firdaus Md Zain, Badrul Munir Yaakop, Salmah Mohd Ridwan, Abd Rahman Aifat, Nor Rahman Mohamed, Mohd Sanusi Maryanto, Ibnu Abdul Latiff, Muhammad Abu Bakar T Technology (General) Dietary assessment plays a crucial role in comprehending the ecological dynamics and nutritional needs of herbivores and omnivores. The metabarcoding technique has emerged as a potent tool for exploring the dietary composition of these animals. However, various metabarcoding techniques have been developed, each with its own advantages and limitations. This study aims to compare the performance of different metabarcoding techniques in herbivores and omnivores diet. We systematically reviewed 159 published manuscripts in Scopus and Google Scholar, and thematic analysis was conducted across several categories, including the marker, platform, and database utilized. Preliminary findings reveal significant variations among metabarcoding techniques across these two animal groups. The trnL gene exhibited higher taxonomic resolution for herbivorous species, whereas the combination of the 'trnL + 16s rRNA' gene exhibited superior performance for omnivorous species. The Illumina platform emerged as the most commonly used method for analyzing the diets of both herbivores and omnivores, with the primary reference database being the National Centre for Biotechnology Information (NCBI). This study offers valuable insights into the strengths and limitations of different metabarcoding techniques for dietary assessment in herbivores and omnivores and optimizing metabarcoding protocols, facilitating more precise and reliable diet analyses within these ecological groups 2023-10-27 Conference or Workshop Item PeerReviewed text en http://eprints.uthm.edu.my/11232/1/P16740_7dd13dbc665a91a58657d0f3e2390e4b%204.pdf Sariyati, Nur Hartini and Haris, Hidayah and Ramli, Farah Farhana and Sufahani, Suliadi Firdaus and Md Zain, Badrul Munir and Yaakop, Salmah and Mohd Ridwan, Abd Rahman and Aifat, Nor Rahman and Mohamed, Mohd Sanusi and Maryanto, Ibnu and Abdul Latiff, Muhammad Abu Bakar (2023) Comparison of metabarcoding techniques for dietary assessment in herbivores and omnivores. In: BIO Web of Conferences. https://doi.org/10.1051/bioconf/20249401002 |
| spellingShingle | T Technology (General) Sariyati, Nur Hartini Haris, Hidayah Ramli, Farah Farhana Sufahani, Suliadi Firdaus Md Zain, Badrul Munir Yaakop, Salmah Mohd Ridwan, Abd Rahman Aifat, Nor Rahman Mohamed, Mohd Sanusi Maryanto, Ibnu Abdul Latiff, Muhammad Abu Bakar Comparison of metabarcoding techniques for dietary assessment in herbivores and omnivores |
| title | Comparison of metabarcoding techniques for dietary assessment in herbivores and omnivores |
| title_full | Comparison of metabarcoding techniques for dietary assessment in herbivores and omnivores |
| title_fullStr | Comparison of metabarcoding techniques for dietary assessment in herbivores and omnivores |
| title_full_unstemmed | Comparison of metabarcoding techniques for dietary assessment in herbivores and omnivores |
| title_short | Comparison of metabarcoding techniques for dietary assessment in herbivores and omnivores |
| title_sort | comparison of metabarcoding techniques for dietary assessment in herbivores and omnivores |
| topic | T Technology (General) |
| url | http://eprints.uthm.edu.my/11232/1/P16740_7dd13dbc665a91a58657d0f3e2390e4b%204.pdf http://eprints.uthm.edu.my/11232/ https://doi.org/10.1051/bioconf/20249401002 |
| url_provider | http://eprints.uthm.edu.my/ |