Enhancing in-house plasmid dna extraction using customized glass fiber membrane
Plasmid DNA extraction and purification from bacterial cells are the foundations of many biotechnological processes. The extracted plasmid can be used for a variety of purposes, including genetic research, forensic analysis, and medical diagnosis. In-house extraction kits that are more cost effectiv...
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| Format: | Monograph |
| Language: | en |
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Universiti Sains Malaysia
2025
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| Online Access: | http://eprints.usm.my/63361/1/JAZMI%20AIMAN%20BIN%20JAMEL-E.pdf http://eprints.usm.my/63361/ |
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| _version_ | 1858062499396452352 |
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| author | Jamel, Jazmi Aiman |
| author_facet | Jamel, Jazmi Aiman |
| author_sort | Jamel, Jazmi Aiman |
| building | Hamzah Sendut Library |
| collection | Institutional Repository |
| content_provider | Universiti Sains Malaysia |
| content_source | USM Institutional Repository |
| continent | Asia |
| country | Malaysia |
| description | Plasmid DNA extraction and purification from bacterial cells are the foundations of many biotechnological processes. The extracted plasmid can be used for a variety of purposes, including genetic research, forensic analysis, and medical diagnosis. In-house extraction kits that are more cost effective and can produce comparable results to the commercial ones are still being pursue by many researchers. In this study we aimed to develop an in-house glass fiber membrane based plasmid extraction kit and compare its yield and purity with commercial kit. The commercial reference kits used in this study will be the Qiagen plasmid extraction kit. The in-house columns with different pore sizes (0.22 μm, 0.45 μm, and 0.80 μm) and number of membrane layers (1, 2 and 6 layers) were self-assembled. The membrane pore size of 0.80 μm yield the highest DNA concentration. the column assembled with 2 layers of GF-0.80 membrane produced highest DNA yield during DNA extraction. The final optimized in-house glass fiber membrane method showed comparable yield and purity (P>0.05) results to the commercial Qiagen plamid DNA extraction kits |
| format | Monograph |
| id | my.usm.eprints.63361 |
| institution | Universiti Sains Malaysia |
| language | en |
| publishDate | 2025 |
| publisher | Universiti Sains Malaysia |
| record_format | eprints |
| spelling | my.usm.eprints.63361 http://eprints.usm.my/63361/ Enhancing in-house plasmid dna extraction using customized glass fiber membrane Jamel, Jazmi Aiman R Medicine RA Public aspects of medicine Plasmid DNA extraction and purification from bacterial cells are the foundations of many biotechnological processes. The extracted plasmid can be used for a variety of purposes, including genetic research, forensic analysis, and medical diagnosis. In-house extraction kits that are more cost effective and can produce comparable results to the commercial ones are still being pursue by many researchers. In this study we aimed to develop an in-house glass fiber membrane based plasmid extraction kit and compare its yield and purity with commercial kit. The commercial reference kits used in this study will be the Qiagen plasmid extraction kit. The in-house columns with different pore sizes (0.22 μm, 0.45 μm, and 0.80 μm) and number of membrane layers (1, 2 and 6 layers) were self-assembled. The membrane pore size of 0.80 μm yield the highest DNA concentration. the column assembled with 2 layers of GF-0.80 membrane produced highest DNA yield during DNA extraction. The final optimized in-house glass fiber membrane method showed comparable yield and purity (P>0.05) results to the commercial Qiagen plamid DNA extraction kits Universiti Sains Malaysia 2025 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/63361/1/JAZMI%20AIMAN%20BIN%20JAMEL-E.pdf Jamel, Jazmi Aiman (2025) Enhancing in-house plasmid dna extraction using customized glass fiber membrane. Project Report. Universiti Sains Malaysia. (Submitted) |
| spellingShingle | R Medicine RA Public aspects of medicine Jamel, Jazmi Aiman Enhancing in-house plasmid dna extraction using customized glass fiber membrane |
| title | Enhancing in-house plasmid dna extraction using customized glass fiber membrane |
| title_full | Enhancing in-house plasmid dna extraction using customized glass fiber membrane |
| title_fullStr | Enhancing in-house plasmid dna extraction using customized glass fiber membrane |
| title_full_unstemmed | Enhancing in-house plasmid dna extraction using customized glass fiber membrane |
| title_short | Enhancing in-house plasmid dna extraction using customized glass fiber membrane |
| title_sort | enhancing in-house plasmid dna extraction using customized glass fiber membrane |
| topic | R Medicine RA Public aspects of medicine |
| url | http://eprints.usm.my/63361/1/JAZMI%20AIMAN%20BIN%20JAMEL-E.pdf http://eprints.usm.my/63361/ |
| url_provider | http://eprints.usm.my/ |