Discrimination between beers with different best before dates using gas chromatography with mass spectrometry
The quality of beer is influenced by various factors, including its best-before dates, which may impact its flavour, aroma, and chemical composition. This study aimed to discriminate between beer samples from different best-before dates and different brands using liquid-liquid extraction followed...
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| Main Author: | |
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| Format: | Thesis |
| Language: | en |
| Published: |
2025
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| Subjects: | |
| Online Access: | http://eprints.usm.my/63140/1/SILKY%20ALEXIS%20ALEX-E.pdf http://eprints.usm.my/63140/ |
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| Summary: | The quality of beer is influenced by various factors, including its best-before
dates, which may impact its flavour, aroma, and chemical composition. This study
aimed to discriminate between beer samples from different best-before dates and
different brands using liquid-liquid extraction followed by gas chromatography with
mass spectrometry (LLE/GC-MS). In this study, beer samples from the brand
Carlsberg® were selected to represent different time points with varying best-before
dates to compare for their GC profiles. Additionally, four beer samples originated from
different brands, namely Carlsberg®, Asahi®, Heineken® and Tiger® were also
analysed and compared for their compositional differences. The GC-MS method was
optimised for solvent selection, dissolution ratio, and injection volume to maximize
detection sensitivity and accuracy, followed by the analyses of beer samples. Key
findings through GC-MS revealed that beers with varying best-before dates exhibited
distinct differences in their chemical profiles. Overall, 35 organic compounds were
identified from Carlsberg® beer sample using the proposed method. Specific profiles
for each beer set and from different brands were observed and visualised through
principal component analysis. To conclude, this study has successfully determined the
chemical profiles of beers with different best-before dates and of different brands,
allowing for forensic discrimination of stale beer samples |
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