Proteomic And Biochemical Analysis Of Detoxification Enzyme In Musca Domestica L. (Diptera: Muscidae) And Its Response Upon Prolonged Exposure Of Insecticide
This study provides the proteomic and biochemical information of the detoxification enzymes namely acetylcholinesterase (AChE), α-esterase (α-est), β- esterase (β-est), glutathione s-transferase (GST) and cytochrome P450 (Cyt P450) in susceptible (adult, larvae and pupae) and poultry strains (lar...
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| Format: | Thesis |
| Language: | en |
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2024
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| Online Access: | http://eprints.usm.my/62045/1/KALAAVATHI%20AP%20MANOHARAN%20-%20TESIS24.pdf http://eprints.usm.my/62045/ |
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| _version_ | 1834507781033426944 |
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| author | Manoharan, Kalaavathi |
| author_facet | Manoharan, Kalaavathi |
| author_sort | Manoharan, Kalaavathi |
| building | Hamzah Sendut Library |
| collection | Institutional Repository |
| content_provider | Universiti Sains Malaysia |
| content_source | USM Institutional Repository |
| continent | Asia |
| country | Malaysia |
| description | This study provides the proteomic and biochemical information of the
detoxification enzymes namely acetylcholinesterase (AChE), α-esterase (α-est), β-
esterase (β-est), glutathione s-transferase (GST) and cytochrome P450 (Cyt P450) in
susceptible (adult, larvae and pupae) and poultry strains (larvae) Musca domestica.
Upon whole protein analysis, number of proteins discovered in larvae was highest
(441), followed by adults (181) and pupae (115) which concluded specific proteins
are necessary for the proper progression of each developmental phase. Meanwhile,
the enzyme activities as well as the protein content of the susceptible strain were
found to have significantly varied enzymatic activity across the developmental stages
(p<0.05). Thirthy four distinct peptides of the targeted detoxification enzyme were
discovered at various developmental stages of the susceptible strain. Functional
analysis of the targeted peptides of the susceptible strains exposed that in addition to
their functions in detoxification, the multifunctional enzymes were also found to be
developmental-related proteins that responsible in various biological process,
molecular functions and KEGG pathways to complete metamorphosis. Overall, it has
been observed that at the larvae stage, the targeted developmental related
detoxification peptides were more expressed and were the most enriched in terms of
biological process, molecular function and KEGG |
| format | Thesis |
| id | my.usm.eprints.62045 |
| institution | Universiti Sains Malaysia |
| language | en |
| publishDate | 2024 |
| record_format | eprints |
| spelling | my.usm.eprints.62045 http://eprints.usm.my/62045/ Proteomic And Biochemical Analysis Of Detoxification Enzyme In Musca Domestica L. (Diptera: Muscidae) And Its Response Upon Prolonged Exposure Of Insecticide Manoharan, Kalaavathi QH1 Natural history (General - Including nature conservation, geographical distribution) This study provides the proteomic and biochemical information of the detoxification enzymes namely acetylcholinesterase (AChE), α-esterase (α-est), β- esterase (β-est), glutathione s-transferase (GST) and cytochrome P450 (Cyt P450) in susceptible (adult, larvae and pupae) and poultry strains (larvae) Musca domestica. Upon whole protein analysis, number of proteins discovered in larvae was highest (441), followed by adults (181) and pupae (115) which concluded specific proteins are necessary for the proper progression of each developmental phase. Meanwhile, the enzyme activities as well as the protein content of the susceptible strain were found to have significantly varied enzymatic activity across the developmental stages (p<0.05). Thirthy four distinct peptides of the targeted detoxification enzyme were discovered at various developmental stages of the susceptible strain. Functional analysis of the targeted peptides of the susceptible strains exposed that in addition to their functions in detoxification, the multifunctional enzymes were also found to be developmental-related proteins that responsible in various biological process, molecular functions and KEGG pathways to complete metamorphosis. Overall, it has been observed that at the larvae stage, the targeted developmental related detoxification peptides were more expressed and were the most enriched in terms of biological process, molecular function and KEGG 2024-02 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/62045/1/KALAAVATHI%20AP%20MANOHARAN%20-%20TESIS24.pdf Manoharan, Kalaavathi (2024) Proteomic And Biochemical Analysis Of Detoxification Enzyme In Musca Domestica L. (Diptera: Muscidae) And Its Response Upon Prolonged Exposure Of Insecticide. Masters thesis, Universiti Sains Malaysia. |
| spellingShingle | QH1 Natural history (General - Including nature conservation, geographical distribution) Manoharan, Kalaavathi Proteomic And Biochemical Analysis Of Detoxification Enzyme In Musca Domestica L. (Diptera: Muscidae) And Its Response Upon Prolonged Exposure Of Insecticide |
| title | Proteomic And Biochemical Analysis
Of Detoxification Enzyme In Musca
Domestica L. (Diptera: Muscidae) And Its
Response Upon Prolonged Exposure Of
Insecticide |
| title_full | Proteomic And Biochemical Analysis
Of Detoxification Enzyme In Musca
Domestica L. (Diptera: Muscidae) And Its
Response Upon Prolonged Exposure Of
Insecticide |
| title_fullStr | Proteomic And Biochemical Analysis
Of Detoxification Enzyme In Musca
Domestica L. (Diptera: Muscidae) And Its
Response Upon Prolonged Exposure Of
Insecticide |
| title_full_unstemmed | Proteomic And Biochemical Analysis
Of Detoxification Enzyme In Musca
Domestica L. (Diptera: Muscidae) And Its
Response Upon Prolonged Exposure Of
Insecticide |
| title_short | Proteomic And Biochemical Analysis
Of Detoxification Enzyme In Musca
Domestica L. (Diptera: Muscidae) And Its
Response Upon Prolonged Exposure Of
Insecticide |
| title_sort | proteomic and biochemical analysis
of detoxification enzyme in musca
domestica l. (diptera: muscidae) and its
response upon prolonged exposure of
insecticide |
| topic | QH1 Natural history (General - Including nature conservation, geographical distribution) |
| url | http://eprints.usm.my/62045/1/KALAAVATHI%20AP%20MANOHARAN%20-%20TESIS24.pdf http://eprints.usm.my/62045/ |
| url_provider | http://eprints.usm.my/ |