HVR-1 Sequence Analysis of Malay Population of Peninsular Malaysia
Sequence of hypervariable 1 (HVS-1) region was analyzed in 37 unrelated Malay population of Peninsular Malaysia. Buccal cell samples were collected from Universiti Sains Malaysia students that came from various parts of Peninsular Malaysia. A 380 bp fragment of HVS-1 region was amplified using a...
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| Format: | Monograph |
| Language: | en |
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Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia
2007
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| Online Access: | http://eprints.usm.my/48230/1/AYU%20SUZALLIANA%20BINTI%20MUHAMAD-24%20pages.pdf http://eprints.usm.my/48230/ |
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| _version_ | 1834504067008692224 |
|---|---|
| author | Muhamad, Ayu Suzailiana |
| author_facet | Muhamad, Ayu Suzailiana |
| author_sort | Muhamad, Ayu Suzailiana |
| building | Hamzah Sendut Library |
| collection | Institutional Repository |
| content_provider | Universiti Sains Malaysia |
| content_source | USM Institutional Repository |
| continent | Asia |
| country | Malaysia |
| description | Sequence of hypervariable 1 (HVS-1) region was analyzed in 37 unrelated
Malay population of Peninsular Malaysia. Buccal cell samples were collected from
Universiti Sains Malaysia students that came from various parts of Peninsular
Malaysia.
A 380 bp fragment of HVS-1 region was amplified using a set of primer, HVA 1
and HVB1. Agarose gel electrophoresis was carried to examine the Polymerase
Chain Reaction (PCR) product and the product was then purified prior to sequencing
using QIAquick PCR Purification kit (QIAGEN). From the total of 37 samples sent for
sequencing, only 25 samples were successfully sequenced.
The sequence for each sample was compared to the Anderson reference
sequence which act as a reference sequence to report for polymorphism; including
total of polymorphic site counted, haplotypes and haplogroup. From result obtained,
52% of the samples were classified under haplogroup M, 24% were classified under
haplogroup F, 16% were classified under haplogroup B and another 8% were
classified under other haplogroups. This result support the finding reported by other
researchers (Schurr and Wallace, 2002; Ballinger eta/., 1992). |
| format | Monograph |
| id | my.usm.eprints.48230 |
| institution | Universiti Sains Malaysia |
| language | en |
| publishDate | 2007 |
| publisher | Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia |
| record_format | eprints |
| spelling | my.usm.eprints.48230 http://eprints.usm.my/48230/ HVR-1 Sequence Analysis of Malay Population of Peninsular Malaysia Muhamad, Ayu Suzailiana R Medicine Sequence of hypervariable 1 (HVS-1) region was analyzed in 37 unrelated Malay population of Peninsular Malaysia. Buccal cell samples were collected from Universiti Sains Malaysia students that came from various parts of Peninsular Malaysia. A 380 bp fragment of HVS-1 region was amplified using a set of primer, HVA 1 and HVB1. Agarose gel electrophoresis was carried to examine the Polymerase Chain Reaction (PCR) product and the product was then purified prior to sequencing using QIAquick PCR Purification kit (QIAGEN). From the total of 37 samples sent for sequencing, only 25 samples were successfully sequenced. The sequence for each sample was compared to the Anderson reference sequence which act as a reference sequence to report for polymorphism; including total of polymorphic site counted, haplotypes and haplogroup. From result obtained, 52% of the samples were classified under haplogroup M, 24% were classified under haplogroup F, 16% were classified under haplogroup B and another 8% were classified under other haplogroups. This result support the finding reported by other researchers (Schurr and Wallace, 2002; Ballinger eta/., 1992). Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia 2007 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/48230/1/AYU%20SUZALLIANA%20BINTI%20MUHAMAD-24%20pages.pdf Muhamad, Ayu Suzailiana (2007) HVR-1 Sequence Analysis of Malay Population of Peninsular Malaysia. Other. Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia, Universiti Sains Malaysia. (Submitted) |
| spellingShingle | R Medicine Muhamad, Ayu Suzailiana HVR-1 Sequence Analysis of Malay Population of Peninsular Malaysia |
| title | HVR-1 Sequence Analysis of Malay Population of Peninsular
Malaysia |
| title_full | HVR-1 Sequence Analysis of Malay Population of Peninsular
Malaysia |
| title_fullStr | HVR-1 Sequence Analysis of Malay Population of Peninsular
Malaysia |
| title_full_unstemmed | HVR-1 Sequence Analysis of Malay Population of Peninsular
Malaysia |
| title_short | HVR-1 Sequence Analysis of Malay Population of Peninsular
Malaysia |
| title_sort | hvr-1 sequence analysis of malay population of peninsular
malaysia |
| topic | R Medicine |
| url | http://eprints.usm.my/48230/1/AYU%20SUZALLIANA%20BINTI%20MUHAMAD-24%20pages.pdf http://eprints.usm.my/48230/ |
| url_provider | http://eprints.usm.my/ |