Evaluation of the potential of Mycobacterium smegmatis as vaccine Candidate against tuberculosis by in silico and in vivo studies
In this study, we scanned multiple published databases of gene expression in vivo of M. tuberculosis at different phases of infection in animals and humans, to select 38 proteins that are highly expressed in the active, latent and reactivation phases. The selected proteins were predicted for T and...
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| Language: | en |
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Finlay Ediciones
2010
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| Online Access: | http://eprints.usm.my/39018/1/Evaluation_of_the_potential_of_Mycobacterium_smegmatis_as_vaccine_Candidate_against_tuberculosis_by_in_silico_and_in_vivo_studies.pdf http://eprints.usm.my/39018/ https://doaj.org/article/2cf44e356c0d4703be0b33c7095b10c1 |
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| author | Nguyen Thi, Le Thuy Maura, Reinier Borrero Férnandez, Sonsire Reyes, Giselle Perez, José Luis Reyes, Fátima García, María de los Angeles Fariñas, Midrey Infante, Juan Francisco Tirado, Yanely Puig, Alina Sierra, Gustavo Álvarez, Nadine Ramírez, Juan Carlos Sarmiento, María Elena Norazmi, Mohd Nor Acosta, Armando |
| author_facet | Nguyen Thi, Le Thuy Maura, Reinier Borrero Férnandez, Sonsire Reyes, Giselle Perez, José Luis Reyes, Fátima García, María de los Angeles Fariñas, Midrey Infante, Juan Francisco Tirado, Yanely Puig, Alina Sierra, Gustavo Álvarez, Nadine Ramírez, Juan Carlos Sarmiento, María Elena Norazmi, Mohd Nor Acosta, Armando |
| author_sort | Nguyen Thi, Le Thuy |
| building | Hamzah Sendut Library |
| collection | Institutional Repository |
| content_provider | Universiti Sains Malaysia |
| content_source | USM Institutional Repository |
| continent | Asia |
| country | Malaysia |
| description | In this study, we scanned multiple published databases of gene expression in vivo of M. tuberculosis at different
phases of infection in animals and humans, to select 38 proteins that are highly expressed in the active, latent
and reactivation phases. The selected proteins were predicted for T and B epitopes. For each proteins, the
regions containing T and B epitopes were selected at the same time to look for identical epitopes on M. smegmatis
based on sequence alignments. Preliminary studies of humoral immunogenicity and cross-reactivity with M.
tuberculosis in mice using two M. smegmatis-derived experimental vaccines were carried out, demonstrating the
immunogenicity of M. smegmatis proteoliposomes and the recognition of M. tuberculosis proteins by the sera of
animals immunized with this vaccine candidate. The conjunction of in silico and in vivo studies suggested the
potential for future evaluation of M. smegmatis as vaccine candidate against tuberculosis using different strategies |
| format | Article |
| id | my.usm.eprints.39018 |
| institution | Universiti Sains Malaysia |
| language | en |
| publishDate | 2010 |
| publisher | Finlay Ediciones |
| record_format | eprints |
| spelling | my.usm.eprints.39018 http://eprints.usm.my/39018/ Evaluation of the potential of Mycobacterium smegmatis as vaccine Candidate against tuberculosis by in silico and in vivo studies Nguyen Thi, Le Thuy Maura, Reinier Borrero Férnandez, Sonsire Reyes, Giselle Perez, José Luis Reyes, Fátima García, María de los Angeles Fariñas, Midrey Infante, Juan Francisco Tirado, Yanely Puig, Alina Sierra, Gustavo Álvarez, Nadine Ramírez, Juan Carlos Sarmiento, María Elena Norazmi, Mohd Nor Acosta, Armando R5-920 Medicine (General) In this study, we scanned multiple published databases of gene expression in vivo of M. tuberculosis at different phases of infection in animals and humans, to select 38 proteins that are highly expressed in the active, latent and reactivation phases. The selected proteins were predicted for T and B epitopes. For each proteins, the regions containing T and B epitopes were selected at the same time to look for identical epitopes on M. smegmatis based on sequence alignments. Preliminary studies of humoral immunogenicity and cross-reactivity with M. tuberculosis in mice using two M. smegmatis-derived experimental vaccines were carried out, demonstrating the immunogenicity of M. smegmatis proteoliposomes and the recognition of M. tuberculosis proteins by the sera of animals immunized with this vaccine candidate. The conjunction of in silico and in vivo studies suggested the potential for future evaluation of M. smegmatis as vaccine candidate against tuberculosis using different strategies Finlay Ediciones 2010 Article PeerReviewed application/pdf en http://eprints.usm.my/39018/1/Evaluation_of_the_potential_of_Mycobacterium_smegmatis_as_vaccine_Candidate_against_tuberculosis_by_in_silico_and_in_vivo_studies.pdf Nguyen Thi, Le Thuy and Maura, Reinier Borrero and Férnandez, Sonsire and Reyes, Giselle and Perez, José Luis and Reyes, Fátima and García, María de los Angeles and Fariñas, Midrey and Infante, Juan Francisco and Tirado, Yanely and Puig, Alina and Sierra, Gustavo and Álvarez, Nadine and Ramírez, Juan Carlos and Sarmiento, María Elena and Norazmi, Mohd Nor and Acosta, Armando (2010) Evaluation of the potential of Mycobacterium smegmatis as vaccine Candidate against tuberculosis by in silico and in vivo studies. VacciMonitor, 19 (1). pp. 20-26. ISSN 1025-028X https://doaj.org/article/2cf44e356c0d4703be0b33c7095b10c1 |
| spellingShingle | R5-920 Medicine (General) Nguyen Thi, Le Thuy Maura, Reinier Borrero Férnandez, Sonsire Reyes, Giselle Perez, José Luis Reyes, Fátima García, María de los Angeles Fariñas, Midrey Infante, Juan Francisco Tirado, Yanely Puig, Alina Sierra, Gustavo Álvarez, Nadine Ramírez, Juan Carlos Sarmiento, María Elena Norazmi, Mohd Nor Acosta, Armando Evaluation of the potential of Mycobacterium smegmatis as vaccine Candidate against tuberculosis by in silico and in vivo studies |
| title | Evaluation of the potential of Mycobacterium smegmatis as vaccine Candidate against tuberculosis by in silico and in vivo studies |
| title_full | Evaluation of the potential of Mycobacterium smegmatis as vaccine Candidate against tuberculosis by in silico and in vivo studies |
| title_fullStr | Evaluation of the potential of Mycobacterium smegmatis as vaccine Candidate against tuberculosis by in silico and in vivo studies |
| title_full_unstemmed | Evaluation of the potential of Mycobacterium smegmatis as vaccine Candidate against tuberculosis by in silico and in vivo studies |
| title_short | Evaluation of the potential of Mycobacterium smegmatis as vaccine Candidate against tuberculosis by in silico and in vivo studies |
| title_sort | evaluation of the potential of mycobacterium smegmatis as vaccine candidate against tuberculosis by in silico and in vivo studies |
| topic | R5-920 Medicine (General) |
| url | http://eprints.usm.my/39018/1/Evaluation_of_the_potential_of_Mycobacterium_smegmatis_as_vaccine_Candidate_against_tuberculosis_by_in_silico_and_in_vivo_studies.pdf http://eprints.usm.my/39018/ https://doaj.org/article/2cf44e356c0d4703be0b33c7095b10c1 |
| url_provider | http://eprints.usm.my/ |