Effects of Calophyllum inophyllum fruit extract on the proliferation and morphological characteristics of human breast cancer cells MCF-7
Objective: To evaluate the antiproliferative activity of Calophyllum inophyllum (C. inophyllum) fruit extract against human breast cancer cells MCF-7. Methods: The cytotoxic effect of C. inophyllum fruit extract against MCF-7 cancer cells was evaluated through MTT and CyQuant assays for 24 h and...
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| Main Authors: | , , , |
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| Format: | Article |
| Published: |
Elsevier
2016
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| Subjects: | |
| Online Access: | http://eprints.usm.my/36912/ https://doi.org/10.1016/S2222-1808(15)61033-7 |
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| Summary: | Objective: To evaluate the antiproliferative activity of Calophyllum inophyllum (C.
inophyllum) fruit extract against human breast cancer cells MCF-7.
Methods: The cytotoxic effect of C. inophyllum fruit extract against MCF-7 cancer cells was
evaluated through MTT and CyQuant assays for 24 h and the morphological investigation of
treated MCF-7 cells was observed under optical microscope using Giemsa staining.
Results: The cytotoxic effect of C. inophyllum fruit extract against MCF-7 cancer cells was
evaluated through MTT and CyQuant assays simultaneously for 24 h after treatment, which
demonstrated the inhibition of cell viability with the IC50 values of 19.63 μg/mL and 27.54
μg/mL, respectively. The preliminary time-based morphological investigation of MCF-7 cells
treated with the IC50 value (23.59 μg/mL) of C. inophyllum fruit extract was observed under an
optical microscopy via Giemsa staining, which exhibited prominent histological characteristics
of apoptosis.
Conclusions: This study clearly proved that the proliferation of human breast cancer cell
MCF-7 was inhibited by C. inophyllum fruit extract resulted from the induction of apoptosis in
MCF-7 cells. |
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