Addition of Low-Cost Additives Improves Real Time PCR Assay of GC Rich FMR1 Region Utilizing Taqman Probe

Objective: To obtain specific amplification, demonstrating that these low-cost additives can improve the amplification of the GC rich region in FMR1 gene, not only in PCR, but Real-time PCR as well. Methods: The basic reaction for the real time PCR were set up in a total volume of 20 ··l containing...

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Bibliographic Details

Main Authors:	Hikmah, E.M., Ankathil, R., Peng, H.B., Zilfalil, B.A.
Format:	Article
Language:	en
Published:	Japan International Cultural Exchange Foundation 2024
Subjects:	real time PCR, FMR1 gene, PCR additives
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