Addition of Low-Cost Additives Improves Real Time PCR Assay of GC Rich FMR1 Region Utilizing Taqman Probe
Objective: To obtain specific amplification, demonstrating that these low-cost additives can improve the amplification of the GC rich region in FMR1 gene, not only in PCR, but Real-time PCR as well. Methods: The basic reaction for the real time PCR were set up in a total volume of 20 ··l containing...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | en |
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Japan International Cultural Exchange Foundation
2024
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