Prevalence and antibiotic susceptibility pattern of staphylococcus aureus, Streptococcus agalactiae and Escherichia coli in dairy goats with clinical and subclinical mastitis
Mastitis is a common disease in lactating goats that widespread throughout the world. This study aims to determine the prevalence of Staphylococcus aureus (S. aureus), Streptococcus agalactiae (S. agalactiae) and Escherichia coli(E. coli) in clinical and subclinical mastitis in does, and to identify...
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| Language: | en |
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Nexus Academic Publishers
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/81821/1/Prevalence%20and%20antibiotic%20susceptibility%20pattern%20of%20staphylococcus%20aureus%2C%20Streptococcus%20agalactiae%20and%20Escherichia%20coli%20in%20dairy%20goats%20with%20clinical%20and%20subclinical%20mastitis.pdf http://psasir.upm.edu.my/id/eprint/81821/ https://pdfs.semanticscholar.org/cd8e/bc4d8196ec13eab39c5113d013de788653d1.pdf |
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| author | Zainal Ariffin, Siti Mariam Hasmadi, Nurhasnida Mohamed Syawari, Nadhirah Sukiman, Muhammad Zikree Tajol Ariffin, Muhammad Faiq Chai, Min Hian Ghazali, Mohd Faizal |
| author_facet | Zainal Ariffin, Siti Mariam Hasmadi, Nurhasnida Mohamed Syawari, Nadhirah Sukiman, Muhammad Zikree Tajol Ariffin, Muhammad Faiq Chai, Min Hian Ghazali, Mohd Faizal |
| author_sort | Zainal Ariffin, Siti Mariam |
| building | UPM Library |
| collection | Institutional Repository |
| content_provider | Universiti Putra Malaysia |
| content_source | UPM Institutional Repository |
| continent | Asia |
| country | Malaysia |
| description | Mastitis is a common disease in lactating goats that widespread throughout the world. This study aims to determine the prevalence of Staphylococcus aureus (S. aureus), Streptococcus agalactiae (S. agalactiae) and Escherichia coli(E. coli) in clinical and subclinical mastitis in does, and to identify the antibiotic susceptibility of the isolated bacteria. A total of 145 milk samples were collected from different farms located around Besut and Setiu districts, Terengganu, Peninsular Malaysia. All does were screened for subclinical mastitis using the California Mastitis Test. Signs of clinical mastitis were recorded. The milk samples were inoculated on Mannitol salt agar, blood agar and Eosin-Methylene Blue agar. The isolated bacterial colonies were subjected to Gram’s staining and biochemical tests (catalase, coagulase, oxidase, triple sugar iron and Christie, Atkins, and Munch-Peterson tests) for identification. The susceptibility of S. aureus, S. agalactiae, and E. coli to the antibiotic was tested using disc diffusion assay. The prevalence of clinical and subclinical mastitis was 23% (34/145) and 40% (58/145) respectively. The bacteriological examinations revealed that 15/92 (16.3%), 6/92 (6.5%) and 1/92 (1.1%) of the mastitis samples were positive for S. aureus, S. agalactiae, and E. coli respectively. Overall resistance levels were very low except for S. aureus towards penicillin (22%) and tetracycline (11%) and for S. agalactiae towards penicillin (33.3%). In conclusion, S. aureus was the most frequently isolated organisms from the caprine mastitis followed by S. agalactiae and E. coli in the study area. |
| format | Article |
| id | my.upm.eprints-81821 |
| institution | Universiti Putra Malaysia |
| language | en |
| publishDate | 2019 |
| publisher | Nexus Academic Publishers |
| record_format | eprints |
| spelling | my.upm.eprints-818212020-09-03T00:17:30Z http://psasir.upm.edu.my/id/eprint/81821/ Prevalence and antibiotic susceptibility pattern of staphylococcus aureus, Streptococcus agalactiae and Escherichia coli in dairy goats with clinical and subclinical mastitis Zainal Ariffin, Siti Mariam Hasmadi, Nurhasnida Mohamed Syawari, Nadhirah Sukiman, Muhammad Zikree Tajol Ariffin, Muhammad Faiq Chai, Min Hian Ghazali, Mohd Faizal Mastitis is a common disease in lactating goats that widespread throughout the world. This study aims to determine the prevalence of Staphylococcus aureus (S. aureus), Streptococcus agalactiae (S. agalactiae) and Escherichia coli(E. coli) in clinical and subclinical mastitis in does, and to identify the antibiotic susceptibility of the isolated bacteria. A total of 145 milk samples were collected from different farms located around Besut and Setiu districts, Terengganu, Peninsular Malaysia. All does were screened for subclinical mastitis using the California Mastitis Test. Signs of clinical mastitis were recorded. The milk samples were inoculated on Mannitol salt agar, blood agar and Eosin-Methylene Blue agar. The isolated bacterial colonies were subjected to Gram’s staining and biochemical tests (catalase, coagulase, oxidase, triple sugar iron and Christie, Atkins, and Munch-Peterson tests) for identification. The susceptibility of S. aureus, S. agalactiae, and E. coli to the antibiotic was tested using disc diffusion assay. The prevalence of clinical and subclinical mastitis was 23% (34/145) and 40% (58/145) respectively. The bacteriological examinations revealed that 15/92 (16.3%), 6/92 (6.5%) and 1/92 (1.1%) of the mastitis samples were positive for S. aureus, S. agalactiae, and E. coli respectively. Overall resistance levels were very low except for S. aureus towards penicillin (22%) and tetracycline (11%) and for S. agalactiae towards penicillin (33.3%). In conclusion, S. aureus was the most frequently isolated organisms from the caprine mastitis followed by S. agalactiae and E. coli in the study area. Nexus Academic Publishers 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/81821/1/Prevalence%20and%20antibiotic%20susceptibility%20pattern%20of%20staphylococcus%20aureus%2C%20Streptococcus%20agalactiae%20and%20Escherichia%20coli%20in%20dairy%20goats%20with%20clinical%20and%20subclinical%20mastitis.pdf Zainal Ariffin, Siti Mariam and Hasmadi, Nurhasnida and Mohamed Syawari, Nadhirah and Sukiman, Muhammad Zikree and Tajol Ariffin, Muhammad Faiq and Chai, Min Hian and Ghazali, Mohd Faizal (2019) Prevalence and antibiotic susceptibility pattern of staphylococcus aureus, Streptococcus agalactiae and Escherichia coli in dairy goats with clinical and subclinical mastitis. Journal of Animal Health and Production, 7 (1). pp. 31-37. ISSN 308-2801 https://pdfs.semanticscholar.org/cd8e/bc4d8196ec13eab39c5113d013de788653d1.pdf 10.17582/journal.jahp/2019/7.1.32.37 |
| spellingShingle | Zainal Ariffin, Siti Mariam Hasmadi, Nurhasnida Mohamed Syawari, Nadhirah Sukiman, Muhammad Zikree Tajol Ariffin, Muhammad Faiq Chai, Min Hian Ghazali, Mohd Faizal Prevalence and antibiotic susceptibility pattern of staphylococcus aureus, Streptococcus agalactiae and Escherichia coli in dairy goats with clinical and subclinical mastitis |
| title | Prevalence and antibiotic susceptibility pattern of staphylococcus aureus, Streptococcus agalactiae and Escherichia coli in dairy goats with clinical and subclinical mastitis |
| title_full | Prevalence and antibiotic susceptibility pattern of staphylococcus aureus, Streptococcus agalactiae and Escherichia coli in dairy goats with clinical and subclinical mastitis |
| title_fullStr | Prevalence and antibiotic susceptibility pattern of staphylococcus aureus, Streptococcus agalactiae and Escherichia coli in dairy goats with clinical and subclinical mastitis |
| title_full_unstemmed | Prevalence and antibiotic susceptibility pattern of staphylococcus aureus, Streptococcus agalactiae and Escherichia coli in dairy goats with clinical and subclinical mastitis |
| title_short | Prevalence and antibiotic susceptibility pattern of staphylococcus aureus, Streptococcus agalactiae and Escherichia coli in dairy goats with clinical and subclinical mastitis |
| title_sort | prevalence and antibiotic susceptibility pattern of staphylococcus aureus, streptococcus agalactiae and escherichia coli in dairy goats with clinical and subclinical mastitis |
| url | http://psasir.upm.edu.my/id/eprint/81821/1/Prevalence%20and%20antibiotic%20susceptibility%20pattern%20of%20staphylococcus%20aureus%2C%20Streptococcus%20agalactiae%20and%20Escherichia%20coli%20in%20dairy%20goats%20with%20clinical%20and%20subclinical%20mastitis.pdf http://psasir.upm.edu.my/id/eprint/81821/ https://pdfs.semanticscholar.org/cd8e/bc4d8196ec13eab39c5113d013de788653d1.pdf |
| url_provider | http://psasir.upm.edu.my/ |