High performance enzymatic synthesis of oleyl oleate using immobilised lipase from Candida antartica
High performance enzymatic synthesis of oleyl oleate, a liquid wax ester was carried out by lipase-catalysed esterification of oleic acid and oleyl alcohol. Various reaction parameters were optimised to obtain high yield of oleyl oleate. The optimum condition to produce oleyl oleate was reaction tim...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | en |
| Published: |
Pontificia Universidad Católica de Valparaíso
2005
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| Online Access: | http://psasir.upm.edu.my/id/eprint/7056/1/High%20performance%20enzymatic%20synthesis%20of%20oleyl%20oleate%20using%20immobilised%20lipase%20from%20Candida%20antartica.pdf http://psasir.upm.edu.my/id/eprint/7056/ http://www.ejbiotechnology.info/index.php/ejbiotechnology/article/view/v8n3-4 |
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| Summary: | High performance enzymatic synthesis of oleyl oleate, a liquid wax ester was carried out by lipase-catalysed esterification of oleic acid and oleyl alcohol. Various reaction parameters were optimised to obtain high yield of oleyl oleate. The optimum condition to produce oleyl oleate was reaction time; 5 min, organic solvents of log P ≥ 3.5, temperature; 40-50ºC, amount of enzyme; 0.2-0.4 g and molar ratio of oleyl alcohol to oleic acid; 2:1. The operational stability of enzyme was maintained at >90% yield up to 9 cycles. Analysis of the yield of the product showed that at optimum conditions, >95% liquid wax esters were produced. |
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