Immature reticulocyte fraction in guiding stem cell harvest in autologous peripheral blood stem cell transplant
Peripheral blood (PB) CD34+ cells enumeration is currently the most reliable method to guide the timing of stem cell harvest. However, its usage is restricted by being technically challenging, costly, and time-consuming. Immature reticulocyte fraction (IRF) determination, which is simpler and cheape...
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Faculty of Medicine and Health Sciences, Universiti Putra Malaysia
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/41385/1/Immature%20reticulocyte%20fraction%20in%20guiding%20stem%20cell.pdf http://psasir.upm.edu.my/id/eprint/41385/ http://www.medic.upm.edu.my/dokumen/FKUSK1_MJMHS_2014V10N1_OP01.pdf |
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| author | Md Noor, Sabariah Leong, Chooi Fun Cheong, Soon Keng |
| author_facet | Md Noor, Sabariah Leong, Chooi Fun Cheong, Soon Keng |
| author_sort | Md Noor, Sabariah |
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| continent | Asia |
| country | Malaysia |
| description | Peripheral blood (PB) CD34+ cells enumeration is currently the most reliable method to guide the timing of stem cell harvest. However, its usage is restricted by being technically challenging, costly, and time-consuming. Immature reticulocyte fraction (IRF) determination, which is simpler and cheaper and has a faster turn-around time, has been proposed for a similar purpose. The purpose of this study is to evaluate the value of IRF in guiding stem cell harvest and examine the correlation between IRF and PB CD34+ cells count. Daily pre-harvest tests, i.e. PB CD34+ cells and IRF from 21patients scheduled for autologous PBSC transplant were assessed. Stem cells harvests were commenced when the PB CD34+ cell count were more than 10 cell/ul. A total of 205 pre-harvest tests were analysed. Following stem cell mobilisations, both the IRF and PB CD 34+ cell counts rose with a variable pattern. In this study, we observed that the IRF peaks preceded the PB CD34+ count by 2 days. On the day of stem cell harvest, all the peak IRF values were >0.3. The PB CD34+ cell counts correlated with the harvested stem cell yield, whereby r2 = 0.77, p < 0.021. In autologous stem cell mobilisation, we believe that IRF is a useful screening tool to predict the rise of the PB CD34+ cell counts as it is a simple, fast and less costly. An IRF of > 0.3 may be used as a cut-off value for the initiation of PB CD34+ quantification prior to stem cell harvest. |
| format | Article |
| id | my.upm.eprints-41385 |
| institution | Universiti Putra Malaysia |
| language | en |
| publishDate | 2014 |
| publisher | Faculty of Medicine and Health Sciences, Universiti Putra Malaysia |
| record_format | eprints |
| spelling | my.upm.eprints-413852015-12-18T01:51:34Z http://psasir.upm.edu.my/id/eprint/41385/ Immature reticulocyte fraction in guiding stem cell harvest in autologous peripheral blood stem cell transplant Md Noor, Sabariah Leong, Chooi Fun Cheong, Soon Keng Peripheral blood (PB) CD34+ cells enumeration is currently the most reliable method to guide the timing of stem cell harvest. However, its usage is restricted by being technically challenging, costly, and time-consuming. Immature reticulocyte fraction (IRF) determination, which is simpler and cheaper and has a faster turn-around time, has been proposed for a similar purpose. The purpose of this study is to evaluate the value of IRF in guiding stem cell harvest and examine the correlation between IRF and PB CD34+ cells count. Daily pre-harvest tests, i.e. PB CD34+ cells and IRF from 21patients scheduled for autologous PBSC transplant were assessed. Stem cells harvests were commenced when the PB CD34+ cell count were more than 10 cell/ul. A total of 205 pre-harvest tests were analysed. Following stem cell mobilisations, both the IRF and PB CD 34+ cell counts rose with a variable pattern. In this study, we observed that the IRF peaks preceded the PB CD34+ count by 2 days. On the day of stem cell harvest, all the peak IRF values were >0.3. The PB CD34+ cell counts correlated with the harvested stem cell yield, whereby r2 = 0.77, p < 0.021. In autologous stem cell mobilisation, we believe that IRF is a useful screening tool to predict the rise of the PB CD34+ cell counts as it is a simple, fast and less costly. An IRF of > 0.3 may be used as a cut-off value for the initiation of PB CD34+ quantification prior to stem cell harvest. Faculty of Medicine and Health Sciences, Universiti Putra Malaysia 2014-01 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/41385/1/Immature%20reticulocyte%20fraction%20in%20guiding%20stem%20cell.pdf Md Noor, Sabariah and Leong, Chooi Fun and Cheong, Soon Keng (2014) Immature reticulocyte fraction in guiding stem cell harvest in autologous peripheral blood stem cell transplant. Malaysian Journal of Medicine and Health Sciences, 10 (1). pp. 1-6. ISSN 1675-8544 http://www.medic.upm.edu.my/dokumen/FKUSK1_MJMHS_2014V10N1_OP01.pdf |
| spellingShingle | Md Noor, Sabariah Leong, Chooi Fun Cheong, Soon Keng Immature reticulocyte fraction in guiding stem cell harvest in autologous peripheral blood stem cell transplant |
| title | Immature reticulocyte fraction in guiding stem cell harvest in autologous peripheral blood stem cell transplant |
| title_full | Immature reticulocyte fraction in guiding stem cell harvest in autologous peripheral blood stem cell transplant |
| title_fullStr | Immature reticulocyte fraction in guiding stem cell harvest in autologous peripheral blood stem cell transplant |
| title_full_unstemmed | Immature reticulocyte fraction in guiding stem cell harvest in autologous peripheral blood stem cell transplant |
| title_short | Immature reticulocyte fraction in guiding stem cell harvest in autologous peripheral blood stem cell transplant |
| title_sort | immature reticulocyte fraction in guiding stem cell harvest in autologous peripheral blood stem cell transplant |
| url | http://psasir.upm.edu.my/id/eprint/41385/1/Immature%20reticulocyte%20fraction%20in%20guiding%20stem%20cell.pdf http://psasir.upm.edu.my/id/eprint/41385/ http://www.medic.upm.edu.my/dokumen/FKUSK1_MJMHS_2014V10N1_OP01.pdf |
| url_provider | http://psasir.upm.edu.my/ |