Method for the purification of interferon.
The present invention discloses a method for the purification of a recombinant microbial Interferon α-2b in an aqueous two-phase system comprising a combination polymer and salt solution. In an embodiment of the present invention, the aqueous two-phase system comprising a combination of polymer and...
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| Main Authors: | , , , |
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| Format: | Patent |
| Published: |
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/32048/ |
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| Summary: | The present invention discloses a method for the purification of a recombinant microbial Interferon α-2b in an aqueous two-phase system comprising a combination polymer and salt solution. In an embodiment of the present invention, the aqueous two-phase system comprising a combination of polymer and salt solution includes polyethylene glycol (PEG)-potassium phosphate, polyethylene glycol (PEG)-ammonium sulfate, polyethylene glycol (PEG)-sodium carbonate, polyethylene glycol (PEG)-sodium citrate and polyethylene glycol (PEG)-magnesium sulfate. The preferred aqueous two-phase system is the polyethylene glycol (PEG)-potassium phosphate system due to the benefits of low cost and short phase separation time as compared to other systems. |
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