Isolation, similarity and subcellular localisation of transaldolase from sugarcane (Saccharum officinarum)
This study focused on isolation, cloning of TAL from sugarcane. Transaldolase is one of the enzymes of the Pentose Phosphate Pathway (PPP). Transaldolase in non-oxidative phase of OPPP transfer a three carbon dihydroxyacetone moiety from sedoheptulose-7-phosphate and glyceraldehyde-3-phophate to pro...
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Medwell Journals
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/30121/1/Isolation.pdf http://psasir.upm.edu.my/id/eprint/30121/ |
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| author | Kalhori, Nahid Nulit, Rosimah Go, Rusea |
| author_facet | Kalhori, Nahid Nulit, Rosimah Go, Rusea |
| author_sort | Kalhori, Nahid |
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| content_provider | Universiti Putra Malaysia |
| content_source | UPM Institutional Repository |
| continent | Asia |
| country | Malaysia |
| description | This study focused on isolation, cloning of TAL from sugarcane. Transaldolase is one of the enzymes of the Pentose Phosphate Pathway (PPP). Transaldolase in non-oxidative phase of OPPP transfer a three carbon dihydroxyacetone moiety from sedoheptulose-7-phosphate and glyceraldehyde-3-phophate to produce Erythrose-4-Phophate (E4P) and fructose-6-phophate. E4P is the precursor for many secondary metabolic pathways including aromatic amino acids, lignin and flavonoid synthesis. Earlier studies revealed that OPPP is incomplete in the cytosol of plants as no genes encoding for a cytosolic TAL. Moreover, there is no study about the TAL genes from sugarcane until to date. Thus, the objective of this study is to isolate TAL gene from sugarcane, to compare its similarity with other plants, to determine its subcellular localization. A total of 1601 bp of TAL has been isolated by PCR. Similarity, studies by ClustalW revealed that TAL show highest similarity (75%) with Zea mays. Analysis of subcellular localization by using Target 1.1 revealed that of TAL from sugarcane was not located in the plastidic. |
| format | Article |
| id | my.upm.eprints-30121 |
| institution | Universiti Putra Malaysia |
| language | en English |
| publishDate | 2013 |
| publisher | Medwell Journals |
| record_format | eprints |
| spelling | my.upm.eprints-301212015-09-18T03:04:53Z http://psasir.upm.edu.my/id/eprint/30121/ Isolation, similarity and subcellular localisation of transaldolase from sugarcane (Saccharum officinarum) Kalhori, Nahid Nulit, Rosimah Go, Rusea This study focused on isolation, cloning of TAL from sugarcane. Transaldolase is one of the enzymes of the Pentose Phosphate Pathway (PPP). Transaldolase in non-oxidative phase of OPPP transfer a three carbon dihydroxyacetone moiety from sedoheptulose-7-phosphate and glyceraldehyde-3-phophate to produce Erythrose-4-Phophate (E4P) and fructose-6-phophate. E4P is the precursor for many secondary metabolic pathways including aromatic amino acids, lignin and flavonoid synthesis. Earlier studies revealed that OPPP is incomplete in the cytosol of plants as no genes encoding for a cytosolic TAL. Moreover, there is no study about the TAL genes from sugarcane until to date. Thus, the objective of this study is to isolate TAL gene from sugarcane, to compare its similarity with other plants, to determine its subcellular localization. A total of 1601 bp of TAL has been isolated by PCR. Similarity, studies by ClustalW revealed that TAL show highest similarity (75%) with Zea mays. Analysis of subcellular localization by using Target 1.1 revealed that of TAL from sugarcane was not located in the plastidic. Medwell Journals 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/30121/1/Isolation.pdf Kalhori, Nahid and Nulit, Rosimah and Go, Rusea (2013) Isolation, similarity and subcellular localisation of transaldolase from sugarcane (Saccharum officinarum). Journal of Molecular Genetics, 5 (1). pp. 1-2. ISSN 2070-4267 English |
| spellingShingle | Kalhori, Nahid Nulit, Rosimah Go, Rusea Isolation, similarity and subcellular localisation of transaldolase from sugarcane (Saccharum officinarum) |
| title | Isolation, similarity and subcellular localisation of transaldolase from sugarcane (Saccharum officinarum) |
| title_full | Isolation, similarity and subcellular localisation of transaldolase from sugarcane (Saccharum officinarum) |
| title_fullStr | Isolation, similarity and subcellular localisation of transaldolase from sugarcane (Saccharum officinarum) |
| title_full_unstemmed | Isolation, similarity and subcellular localisation of transaldolase from sugarcane (Saccharum officinarum) |
| title_short | Isolation, similarity and subcellular localisation of transaldolase from sugarcane (Saccharum officinarum) |
| title_sort | isolation, similarity and subcellular localisation of transaldolase from sugarcane (saccharum officinarum) |
| url | http://psasir.upm.edu.my/id/eprint/30121/1/Isolation.pdf http://psasir.upm.edu.my/id/eprint/30121/ |
| url_provider | http://psasir.upm.edu.my/ |