Extraction of total RNA and identification of different genes in mangrove plant involved in its adaptability to the variety of stresses
Nucleic acids extraction from mangroves as woody plants needs lots of practical experience. In the present study, three different methods and the RNeasy plant mini kit were used to extract nucleic acids from mangrove plant. Modified CTAB method provided high integrity and concentration of DNA and RN...
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| Format: | Article |
| Language: | en |
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University of Agriculture, Faisalabad, Pakistan
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/29421/1/29421.pdf http://psasir.upm.edu.my/id/eprint/29421/ http://www.pakjas.com.pk/viewpapers.aspx?valv=50&vali=133 |
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| author | Sahebi, Mahbob Musa, Mohamed Hanafi Abdullah, Siti Nor Akmal Nejat, Naghmeh Yusop, Mohd Rafii Azizi, Parisa |
| author_facet | Sahebi, Mahbob Musa, Mohamed Hanafi Abdullah, Siti Nor Akmal Nejat, Naghmeh Yusop, Mohd Rafii Azizi, Parisa |
| author_sort | Sahebi, Mahbob |
| building | UPM Library |
| collection | Institutional Repository |
| content_provider | Universiti Putra Malaysia |
| content_source | UPM Institutional Repository |
| continent | Asia |
| country | Malaysia |
| description | Nucleic acids extraction from mangroves as woody plants needs lots of practical experience. In the present study, three different methods and the RNeasy plant mini kit were used to extract nucleic acids from mangrove plant. Modified CTAB method provided high integrity and concentration of DNA and RNA, respectively from roots and leaves. This method was successful in extraction of RNA from roots but failed for leaves of mangrove. The CTAB with β-mercaptoethanol increases RNA concentration while it decreases the purity of RNA. High yield of RNA extracted from mangrove leaves was obtained by modifying the SDS method. However, the integrity of RNA decreased. When polyvinylpyrrolidone was used to inhibit polyphenol oxidase activity, the yield and integrity of RNA had improved significantly. Therefore, the objective of this study was to isolate and identify the different genes involved in the adaptation of mangrove plants to different kind of stresses. Two-month old seedlings of the Rhizophoraapiculata were exposed to 450 mM NaCl for 24 hours under hydroponic culture. Then, the expression of dihydrolipoamide dehydrogenase (DLDH) gene was studied using reverse transcriptase-PCR and real-time qRT-PCR. |
| format | Article |
| id | my.upm.eprints-29421 |
| institution | Universiti Putra Malaysia |
| language | en |
| publishDate | 2013 |
| publisher | University of Agriculture, Faisalabad, Pakistan |
| record_format | eprints |
| spelling | my.upm.eprints-294212016-07-12T09:29:42Z http://psasir.upm.edu.my/id/eprint/29421/ Extraction of total RNA and identification of different genes in mangrove plant involved in its adaptability to the variety of stresses Sahebi, Mahbob Musa, Mohamed Hanafi Abdullah, Siti Nor Akmal Nejat, Naghmeh Yusop, Mohd Rafii Azizi, Parisa Nucleic acids extraction from mangroves as woody plants needs lots of practical experience. In the present study, three different methods and the RNeasy plant mini kit were used to extract nucleic acids from mangrove plant. Modified CTAB method provided high integrity and concentration of DNA and RNA, respectively from roots and leaves. This method was successful in extraction of RNA from roots but failed for leaves of mangrove. The CTAB with β-mercaptoethanol increases RNA concentration while it decreases the purity of RNA. High yield of RNA extracted from mangrove leaves was obtained by modifying the SDS method. However, the integrity of RNA decreased. When polyvinylpyrrolidone was used to inhibit polyphenol oxidase activity, the yield and integrity of RNA had improved significantly. Therefore, the objective of this study was to isolate and identify the different genes involved in the adaptation of mangrove plants to different kind of stresses. Two-month old seedlings of the Rhizophoraapiculata were exposed to 450 mM NaCl for 24 hours under hydroponic culture. Then, the expression of dihydrolipoamide dehydrogenase (DLDH) gene was studied using reverse transcriptase-PCR and real-time qRT-PCR. University of Agriculture, Faisalabad, Pakistan 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29421/1/29421.pdf Sahebi, Mahbob and Musa, Mohamed Hanafi and Abdullah, Siti Nor Akmal and Nejat, Naghmeh and Yusop, Mohd Rafii and Azizi, Parisa (2013) Extraction of total RNA and identification of different genes in mangrove plant involved in its adaptability to the variety of stresses. Pakistan Journal of Agricultural Sciences, 50 (4). pp. 529-536. ISSN 0552-9034; ESSN: 2076-0906 http://www.pakjas.com.pk/viewpapers.aspx?valv=50&vali=133 |
| spellingShingle | Sahebi, Mahbob Musa, Mohamed Hanafi Abdullah, Siti Nor Akmal Nejat, Naghmeh Yusop, Mohd Rafii Azizi, Parisa Extraction of total RNA and identification of different genes in mangrove plant involved in its adaptability to the variety of stresses |
| title | Extraction of total RNA and identification of different genes in mangrove plant involved in its adaptability to the variety of stresses |
| title_full | Extraction of total RNA and identification of different genes in mangrove plant involved in its adaptability to the variety of stresses |
| title_fullStr | Extraction of total RNA and identification of different genes in mangrove plant involved in its adaptability to the variety of stresses |
| title_full_unstemmed | Extraction of total RNA and identification of different genes in mangrove plant involved in its adaptability to the variety of stresses |
| title_short | Extraction of total RNA and identification of different genes in mangrove plant involved in its adaptability to the variety of stresses |
| title_sort | extraction of total rna and identification of different genes in mangrove plant involved in its adaptability to the variety of stresses |
| url | http://psasir.upm.edu.my/id/eprint/29421/1/29421.pdf http://psasir.upm.edu.my/id/eprint/29421/ http://www.pakjas.com.pk/viewpapers.aspx?valv=50&vali=133 |
| url_provider | http://psasir.upm.edu.my/ |