Molecular identification of Candida orthopsilosis isolated from blood culture.
The incidence of candidemia and invasive candidiasis have increased markedly due to the increasing number of immunocompromised patients. There are five major medically important species of Candida with their frequency of isolation in the diminishing order namely Candida albicans, Candida parapsilosi...
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Springer Verlag
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/15708/1/Molecular%20identification%20of%20Candida%20orthopsilosis%20isolated%20from%20blood%20culture.pdf http://psasir.upm.edu.my/id/eprint/15708/ http://link.springer.com/ |
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| author | Yong, P.V.C. Chong, Pei Pei Lau, L.Y. Yeoh, R.S.C. Jamal, Farida |
| author_facet | Yong, P.V.C. Chong, Pei Pei Lau, L.Y. Yeoh, R.S.C. Jamal, Farida |
| author_sort | Yong, P.V.C. |
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| content_provider | Universiti Putra Malaysia |
| content_source | UPM Institutional Repository |
| continent | Asia |
| country | Malaysia |
| description | The incidence of candidemia and invasive candidiasis have increased markedly due to the increasing number of immunocompromised patients. There are five major medically important species of Candida with their frequency of isolation in the diminishing order namely Candida albicans, Candida parapsilosis, Candida tropicalis, Candida glabrata and Candida krusei. In addition, there are numerous other species of Candida which differ in their genetic makeup, virulence properties, drug susceptibilities and sugar assimilation capabilities. In this report, an unusual Candida species was isolated from the blood of two leukaemic patients. Conventional culture and biochemical tests identified the Candida species as C. parapsilosis. Using fungal-specific oligonucleotide primers ITS1 and ITS4, we managed to amplify the ribosomal RNA gene and its internal transcribed spacer region from the genomic DNA of these isolates. The PCR products were then purified and subjected to automated DNA sequencing using BLAST and CLUSTAL sequence analysis identified these isolates to be Candida orthopsilosis. Candida orthopsilosis is a new species recently identified in 2005, being morphologically indistinguishable from C. parapsilosis and was previously classified as a subspecies of C. parapsilosis. This report highlights the importance of complementing traditional culture and biochemical-based identification methods with DNA-based molecular assays such as PCR as the latter is more superior in terms of its discriminatory power and speed. |
| format | Article |
| id | my.upm.eprints-15708 |
| institution | Universiti Putra Malaysia |
| language | en English |
| publishDate | 2008 |
| publisher | Springer Verlag |
| record_format | eprints |
| spelling | my.upm.eprints-157082015-11-11T00:53:30Z http://psasir.upm.edu.my/id/eprint/15708/ Molecular identification of Candida orthopsilosis isolated from blood culture. Yong, P.V.C. Chong, Pei Pei Lau, L.Y. Yeoh, R.S.C. Jamal, Farida The incidence of candidemia and invasive candidiasis have increased markedly due to the increasing number of immunocompromised patients. There are five major medically important species of Candida with their frequency of isolation in the diminishing order namely Candida albicans, Candida parapsilosis, Candida tropicalis, Candida glabrata and Candida krusei. In addition, there are numerous other species of Candida which differ in their genetic makeup, virulence properties, drug susceptibilities and sugar assimilation capabilities. In this report, an unusual Candida species was isolated from the blood of two leukaemic patients. Conventional culture and biochemical tests identified the Candida species as C. parapsilosis. Using fungal-specific oligonucleotide primers ITS1 and ITS4, we managed to amplify the ribosomal RNA gene and its internal transcribed spacer region from the genomic DNA of these isolates. The PCR products were then purified and subjected to automated DNA sequencing using BLAST and CLUSTAL sequence analysis identified these isolates to be Candida orthopsilosis. Candida orthopsilosis is a new species recently identified in 2005, being morphologically indistinguishable from C. parapsilosis and was previously classified as a subspecies of C. parapsilosis. This report highlights the importance of complementing traditional culture and biochemical-based identification methods with DNA-based molecular assays such as PCR as the latter is more superior in terms of its discriminatory power and speed. Springer Verlag 2008-02 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15708/1/Molecular%20identification%20of%20Candida%20orthopsilosis%20isolated%20from%20blood%20culture.pdf Yong, P.V.C. and Chong, Pei Pei and Lau, L.Y. and Yeoh, R.S.C. and Jamal, Farida (2008) Molecular identification of Candida orthopsilosis isolated from blood culture. Mycopathologia, 165 (2 ). pp. 81-87. ISSN 0301-486X; ESSN: 1573-0832 http://link.springer.com/ 10.1007/s11046-007-9086-8 English |
| spellingShingle | Yong, P.V.C. Chong, Pei Pei Lau, L.Y. Yeoh, R.S.C. Jamal, Farida Molecular identification of Candida orthopsilosis isolated from blood culture. |
| title | Molecular identification of Candida orthopsilosis isolated from blood culture. |
| title_full | Molecular identification of Candida orthopsilosis isolated from blood culture. |
| title_fullStr | Molecular identification of Candida orthopsilosis isolated from blood culture. |
| title_full_unstemmed | Molecular identification of Candida orthopsilosis isolated from blood culture. |
| title_short | Molecular identification of Candida orthopsilosis isolated from blood culture. |
| title_sort | molecular identification of candida orthopsilosis isolated from blood culture. |
| url | http://psasir.upm.edu.my/id/eprint/15708/1/Molecular%20identification%20of%20Candida%20orthopsilosis%20isolated%20from%20blood%20culture.pdf http://psasir.upm.edu.my/id/eprint/15708/ http://link.springer.com/ |
| url_provider | http://psasir.upm.edu.my/ |