An aqueous extract of Citrus mitis possess antioxidative properties and improves plasma lipid profiles in rat induced with high cholesterol diet
The in vitro antioxidant activity of Citrus mitis aqueous extract (CME) and its effects on antioxidative status and lipid profiles of rat fed with high cholesterol diet were examined. The in vitro antioxidant activity was assessed by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferric-reducing antioxid...
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Academic Journals
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/13021/1/An%20aqueous%20extract%20of%20Citrus%20mitis%20possess%20antioxidative%20properties%20and%20improves%20plasma%20lipid%20profiles%20in%20rat%20induced%20with%20high%20cholesterol%20diet.pdf http://psasir.upm.edu.my/id/eprint/13021/ http://www.academicjournals.org/journal/JMPR/article-abstract/AF5BB5314895 |
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| author | Amom, Zulkhairi Fairuz Azman, Khairunnuur Md Rasdi, Nor Hafipah Azlan, Azrina Mat Ali, Rasadah Abdul Kadir, Khairul Kamilah Md. Shah, Zamree Mohamad Arshad, Mohd Shahidan |
| author_facet | Amom, Zulkhairi Fairuz Azman, Khairunnuur Md Rasdi, Nor Hafipah Azlan, Azrina Mat Ali, Rasadah Abdul Kadir, Khairul Kamilah Md. Shah, Zamree Mohamad Arshad, Mohd Shahidan |
| author_sort | Amom, Zulkhairi |
| building | UPM Library |
| collection | Institutional Repository |
| content_provider | Universiti Putra Malaysia |
| content_source | UPM Institutional Repository |
| continent | Asia |
| country | Malaysia |
| description | The in vitro antioxidant activity of Citrus mitis aqueous extract (CME) and its effects on antioxidative status and lipid profiles of rat fed with high cholesterol diet were examined. The in vitro antioxidant activity was assessed by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferric-reducing antioxidant power (FRAP), while the total phenolic content was measured as gallic acid equivalent. The antioxidative status in the plasma was further assessed by thiobarbituric acid reactive substances (TBARS) assay whereas plasma lipid profile was analysed spectrophotometrically. The result showed that both 5 and 10% extracts possessed antioxidant activities in concentration dependent manner in all tested methods which positively correlated with high phenolic content. The supplementation of 5 mg/kg of both 5 and 10% CME respectively reduced plasma total cholesterol (TCHOL), low-density lipoprotein (LDL) and triglycerides (TG) levels concomitantly with an increased level of high-density lipoprotein (HDL) in rat induced hypercholesterolemia (p < 0.05). The lipid parameters were comparable with statin. The atherogenic index (AI) and sdLDL values were found to be lower in CME-treated groups compared to the control (p < 0.05). Microsomal lipid peroxidation indicated with TBARS estimation was found to be lower in both CME-treated groups. The results obtained suggest that C. mitisaqueous extract possesses lipid lowering and antioxidative effect in hypercholesterolemic-induced model and could potentially be used as therapeutic regiment in managing hypercholesterolemia. |
| format | Article |
| id | my.upm.eprints-13021 |
| institution | Universiti Putra Malaysia |
| language | en |
| publishDate | 2010 |
| publisher | Academic Journals |
| record_format | eprints |
| spelling | my.upm.eprints-130212016-06-06T05:28:43Z http://psasir.upm.edu.my/id/eprint/13021/ An aqueous extract of Citrus mitis possess antioxidative properties and improves plasma lipid profiles in rat induced with high cholesterol diet Amom, Zulkhairi Fairuz Azman, Khairunnuur Md Rasdi, Nor Hafipah Azlan, Azrina Mat Ali, Rasadah Abdul Kadir, Khairul Kamilah Md. Shah, Zamree Mohamad Arshad, Mohd Shahidan The in vitro antioxidant activity of Citrus mitis aqueous extract (CME) and its effects on antioxidative status and lipid profiles of rat fed with high cholesterol diet were examined. The in vitro antioxidant activity was assessed by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferric-reducing antioxidant power (FRAP), while the total phenolic content was measured as gallic acid equivalent. The antioxidative status in the plasma was further assessed by thiobarbituric acid reactive substances (TBARS) assay whereas plasma lipid profile was analysed spectrophotometrically. The result showed that both 5 and 10% extracts possessed antioxidant activities in concentration dependent manner in all tested methods which positively correlated with high phenolic content. The supplementation of 5 mg/kg of both 5 and 10% CME respectively reduced plasma total cholesterol (TCHOL), low-density lipoprotein (LDL) and triglycerides (TG) levels concomitantly with an increased level of high-density lipoprotein (HDL) in rat induced hypercholesterolemia (p < 0.05). The lipid parameters were comparable with statin. The atherogenic index (AI) and sdLDL values were found to be lower in CME-treated groups compared to the control (p < 0.05). Microsomal lipid peroxidation indicated with TBARS estimation was found to be lower in both CME-treated groups. The results obtained suggest that C. mitisaqueous extract possesses lipid lowering and antioxidative effect in hypercholesterolemic-induced model and could potentially be used as therapeutic regiment in managing hypercholesterolemia. Academic Journals 2010-01 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/13021/1/An%20aqueous%20extract%20of%20Citrus%20mitis%20possess%20antioxidative%20properties%20and%20improves%20plasma%20lipid%20profiles%20in%20rat%20induced%20with%20high%20cholesterol%20diet.pdf Amom, Zulkhairi and Fairuz Azman, Khairunnuur and Md Rasdi, Nor Hafipah and Azlan, Azrina and Mat Ali, Rasadah and Abdul Kadir, Khairul Kamilah and Md. Shah, Zamree and Mohamad Arshad, Mohd Shahidan (2010) An aqueous extract of Citrus mitis possess antioxidative properties and improves plasma lipid profiles in rat induced with high cholesterol diet. Journal of Medicinal Plants Research, 4 (1). art. no. AF5BB5314895. pp. 49-57. ISSN 1996-0875 http://www.academicjournals.org/journal/JMPR/article-abstract/AF5BB5314895 |
| spellingShingle | Amom, Zulkhairi Fairuz Azman, Khairunnuur Md Rasdi, Nor Hafipah Azlan, Azrina Mat Ali, Rasadah Abdul Kadir, Khairul Kamilah Md. Shah, Zamree Mohamad Arshad, Mohd Shahidan An aqueous extract of Citrus mitis possess antioxidative properties and improves plasma lipid profiles in rat induced with high cholesterol diet |
| title | An aqueous extract of Citrus mitis possess antioxidative properties and improves plasma lipid profiles in rat induced with high cholesterol diet |
| title_full | An aqueous extract of Citrus mitis possess antioxidative properties and improves plasma lipid profiles in rat induced with high cholesterol diet |
| title_fullStr | An aqueous extract of Citrus mitis possess antioxidative properties and improves plasma lipid profiles in rat induced with high cholesterol diet |
| title_full_unstemmed | An aqueous extract of Citrus mitis possess antioxidative properties and improves plasma lipid profiles in rat induced with high cholesterol diet |
| title_short | An aqueous extract of Citrus mitis possess antioxidative properties and improves plasma lipid profiles in rat induced with high cholesterol diet |
| title_sort | aqueous extract of citrus mitis possess antioxidative properties and improves plasma lipid profiles in rat induced with high cholesterol diet |
| url | http://psasir.upm.edu.my/id/eprint/13021/1/An%20aqueous%20extract%20of%20Citrus%20mitis%20possess%20antioxidative%20properties%20and%20improves%20plasma%20lipid%20profiles%20in%20rat%20induced%20with%20high%20cholesterol%20diet.pdf http://psasir.upm.edu.my/id/eprint/13021/ http://www.academicjournals.org/journal/JMPR/article-abstract/AF5BB5314895 |
| url_provider | http://psasir.upm.edu.my/ |