Methanol response of V171S lipase from Geobacillus sp. 42 explored via experimental and in silico approaches

The stability of lipase in organic solvents is crucial for biocatalytic processes in industrial biotechnology. Previously, we described a thermostable and solvent-tolerant Lip 42 from Geobacillus sp. Despite these features, the wild-type Lip 42 activity deteriorated and became unstable in methanol a...

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Bibliographic Details

Main Authors:	Mohd Johan, Ummie Umaiera, Ishak, Siti Nor Hasmah, Latip, Wahhida, Raja Abd Rahman, Raja Noor Zaliha, Salleh, Abu Bakar, Leow, Adam Thean Chor, Mohamad Ali, Mohd Shukuri
Format:	Article
Language:	en
Published:	Springer Science and Business Media Deutschland GmbH 2025
Subjects:	Biophysics Applied Microbiology and Biotechnology
Online Access:	http://psasir.upm.edu.my/id/eprint/124546/1/124546.pdf http://psasir.upm.edu.my/id/eprint/124546/ https://link.springer.com/article/10.1186/s13568-025-01966-7
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