Alginate and Spirulina vast-promoting immune activity and resistance to diverse Vibrio species in Litopenaeus vannamei culture
Litopenaeus vannamei is a key species in global aquaculture; however, its susceptibility to vibriosis presents a persistent challenge. The aim of this study was to investigate the effects of dietary supplementation with alginate and Spirulina hot water extract (SWE) on the immune response and gene e...
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Diponegoro University
2026
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| Online Access: | http://psasir.upm.edu.my/id/eprint/123789/1/123789.pdf http://psasir.upm.edu.my/id/eprint/123789/ https://ejournal.undip.ac.id/index.php/ijms/article/view/80383 |
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| author | Yudiati, Ervia Trianto, Agus Sarjito Utami, Diah Ayu Satyari Karim, Murni Azhar, Nuril |
| author_facet | Yudiati, Ervia Trianto, Agus Sarjito Utami, Diah Ayu Satyari Karim, Murni Azhar, Nuril |
| author_sort | Yudiati, Ervia |
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| content_provider | Universiti Putra Malaysia |
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| continent | Asia |
| country | Malaysia |
| description | Litopenaeus vannamei is a key species in global aquaculture; however, its susceptibility to vibriosis presents a persistent challenge. The aim of this study was to investigate the effects of dietary supplementation with alginate and Spirulina hot water extract (SWE) on the immune response and gene expression associated with pathogen resistance. Shrimp fed diets containing 3 g·kg-1 of alginate and 5 mg·kg-1 of SWE exhibited marked improvements in immune parameters, including total hemocyte count, phagocytic activity, phagocytic index, phenoloxidase activity, and superoxide dismutase levels, as measured on days 3 and 7 (P<0.05). Following challenge with Vibrio parahaemolyticus, V. vulnificus, and V. harveyi, treated shrimp demonstrated a 90% survival rate compared to 0% in the control group. Gene expression analysis revealed a 2-fold and 1.5-fold upregulation of prophenoloxidase (proPO) and lipopolysaccharide β-glucan binding protein (LGBP), respectively, indicating the activation of the proPO cascade and pathogen recognition pathways. These results provide direct evidence that alginate and SWE act as immunostimulants by modulating hemocyte function and immune-related gene expression. The findings of the present study suggest that oral supplementation with these natural bioactive compounds can serve as a functional dietary strategy to enhance disease resilience in shrimp and promote more sustainable aquaculture systems, in seven days period. |
| format | Article |
| id | my.upm.eprints-123789 |
| institution | Universiti Putra Malaysia |
| language | en |
| publishDate | 2026 |
| publisher | Diponegoro University |
| record_format | eprints |
| spelling | my.upm.eprints-1237892026-04-13T00:34:52Z http://psasir.upm.edu.my/id/eprint/123789/ Alginate and Spirulina vast-promoting immune activity and resistance to diverse Vibrio species in Litopenaeus vannamei culture Yudiati, Ervia Trianto, Agus Sarjito Utami, Diah Ayu Satyari Karim, Murni Azhar, Nuril Litopenaeus vannamei is a key species in global aquaculture; however, its susceptibility to vibriosis presents a persistent challenge. The aim of this study was to investigate the effects of dietary supplementation with alginate and Spirulina hot water extract (SWE) on the immune response and gene expression associated with pathogen resistance. Shrimp fed diets containing 3 g·kg-1 of alginate and 5 mg·kg-1 of SWE exhibited marked improvements in immune parameters, including total hemocyte count, phagocytic activity, phagocytic index, phenoloxidase activity, and superoxide dismutase levels, as measured on days 3 and 7 (P<0.05). Following challenge with Vibrio parahaemolyticus, V. vulnificus, and V. harveyi, treated shrimp demonstrated a 90% survival rate compared to 0% in the control group. Gene expression analysis revealed a 2-fold and 1.5-fold upregulation of prophenoloxidase (proPO) and lipopolysaccharide β-glucan binding protein (LGBP), respectively, indicating the activation of the proPO cascade and pathogen recognition pathways. These results provide direct evidence that alginate and SWE act as immunostimulants by modulating hemocyte function and immune-related gene expression. The findings of the present study suggest that oral supplementation with these natural bioactive compounds can serve as a functional dietary strategy to enhance disease resilience in shrimp and promote more sustainable aquaculture systems, in seven days period. Diponegoro University 2026-03-01 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/123789/1/123789.pdf Yudiati, Ervia and Trianto, Agus and Sarjito and Utami, Diah Ayu Satyari and Karim, Murni and Azhar, Nuril (2026) Alginate and Spirulina vast-promoting immune activity and resistance to diverse Vibrio species in Litopenaeus vannamei culture. Ilmu Kelautan: Indonesian Journal of Marine Sciences, 31 (1). pp. 49-60. ISSN 0853-7291; eISSN: 2406-7598 https://ejournal.undip.ac.id/index.php/ijms/article/view/80383 Oceanography Aquatic Science Ecology 10.14710/ik.ijms.31.1.49-60 |
| spellingShingle | Oceanography Aquatic Science Ecology Yudiati, Ervia Trianto, Agus Sarjito Utami, Diah Ayu Satyari Karim, Murni Azhar, Nuril Alginate and Spirulina vast-promoting immune activity and resistance to diverse Vibrio species in Litopenaeus vannamei culture |
| title | Alginate and Spirulina vast-promoting immune activity and resistance to diverse Vibrio species in Litopenaeus vannamei culture |
| title_full | Alginate and Spirulina vast-promoting immune activity and resistance to diverse Vibrio species in Litopenaeus vannamei culture |
| title_fullStr | Alginate and Spirulina vast-promoting immune activity and resistance to diverse Vibrio species in Litopenaeus vannamei culture |
| title_full_unstemmed | Alginate and Spirulina vast-promoting immune activity and resistance to diverse Vibrio species in Litopenaeus vannamei culture |
| title_short | Alginate and Spirulina vast-promoting immune activity and resistance to diverse Vibrio species in Litopenaeus vannamei culture |
| title_sort | alginate and spirulina vast-promoting immune activity and resistance to diverse vibrio species in litopenaeus vannamei culture |
| topic | Oceanography Aquatic Science Ecology |
| url | http://psasir.upm.edu.my/id/eprint/123789/1/123789.pdf http://psasir.upm.edu.my/id/eprint/123789/ https://ejournal.undip.ac.id/index.php/ijms/article/view/80383 |
| url_provider | http://psasir.upm.edu.my/ |