Insight into improved specificity and thermostability of Geobacillus zalihae T1 lipase by introducing novel molecular interactions via phenylalanin to cysteine substitution

Insight into improved specificity and thermostability of Geobacillus zalihae T1 lipase by introducing novel molecular interactions via phenylalanin to cysteine substitution

Lipase specificity is a crucial requirement for industrial employment; however, tuning the lipase specificity in some cases can impair the structure and affect its stability. To enhance the T1 lipase specificity, we targeted the conserved bulky residue Phe180 in the lid domain to eliminate the impac...

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Main Authors: Albayati, Samah Hashim, Shabeeb, Muna Hilal, Akinola, Oluwasola Michael, Nezhad, Nima Ghahremani, Chor, Adam Leow Thean, Mohamad Ali, Mohd Shukuri, Mohd Shariff, Fairolniza, Muhd Noor, Noor Dina, Abd Rahman, Raja Noor Zaliha Raja
Format: Article
Language:en
Published: Springer Science and Business Media Deutschland GmbH 2025
Online Access:http://psasir.upm.edu.my/id/eprint/121282/1/121282.pdf
http://psasir.upm.edu.my/id/eprint/121282/
https://link.springer.com/article/10.1007/s13205-025-04330-5?error=cookies_not_supported&code=2595568d-be69-4366-80df-11410a1cb094
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http://psasir.upm.edu.my/id/eprint/121282/1/121282.pdf
http://psasir.upm.edu.my/id/eprint/121282/
https://link.springer.com/article/10.1007/s13205-025-04330-5?error=cookies_not_supported&code=2595568d-be69-4366-80df-11410a1cb094

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