Caspases-3 and -7 are activated in goniothalamin-induced apoptosis in human Jurkat T-cells
Goniothalamin, a plant styrylpyrone derivative isolated from Goniothalamus andersonii, induced apoptosis in Jurkat T-cells as assessed by the externalisation of phosphatidylserine. Immunoblotting showed processing of caspases-3 and -7 with the appearance of their catalytically active large subunits...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | en |
| Published: |
Federation of European Biochemical Societies
1999
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| Online Access: | http://psasir.upm.edu.my/id/eprint/114448/1/114448.pdf http://psasir.upm.edu.my/id/eprint/114448/ https://febs.onlinelibrary.wiley.com/doi/10.1016/S0014-5793%2899%2900984-9 |
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| Summary: | Goniothalamin, a plant styrylpyrone derivative isolated from Goniothalamus andersonii, induced apoptosis in Jurkat T-cells as assessed by the externalisation of phosphatidylserine. Immunoblotting showed processing of caspases-3 and -7 with the appearance of their catalytically active large subunits of 17 and 19 kDa, respectively. Activation of these caspases was further evidenced by detection of poly(ADP-ribose) polymerase cleavage (PARP). Pre-treatment with the caspase inhibitor benzyloxycarbonyl-Val-Ala-Asp fluoromethyl ketone (Z-VAD.FMK) blocked apoptosis and the resultant cleavage of these caspases and PARP. Our results demonstrate that activation of at least two effector caspases is a key feature of goniothalamin-induced apoptosis in Jurkat T-cells. |
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